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Citations & References (285)
Invitrogen™
Lipofectin™ Transfection Reagent
Lipofectin® Transfection Reagent is the reagent of choice for transfection of endothelial cells. Lipofectin® Transfection Reagent is also suitable forRead more
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| Catalog Number | Quantity |
|---|---|
| 18292037 | 4 mL |
| 18292011 | 1 mL |
Catalog number 18292037
Price (CNY)
26,368.00
Each
Quantity:
4 mL
Price (CNY)
26,368.00
Each
Lipofectin® Transfection Reagent is the reagent of choice for transfection of endothelial cells. Lipofectin® Transfection Reagent is also suitable for transfecting DNA, RNA, and oligonucleotides into mammalian cells, and DNA into plant protoplasts. Lipofectin® reagent has also been shown to work well, in combination with PLUS® Reagent, for the transfection of HeLa cells. Lipofectin® Transfection Reagent is a 1:1 (w/w) liposome formulation of the cationic lipid N-[1-(2,3-dioleyloxy)propyl]-n,n,n-trimethylammonium chloride (DOTMA) and dioleoyl phophotidylethanolamine (DOPE) in membrane-filtered water.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Transfection
High-throughput CompatibilityNot High-throughput Compatible (Manual)
IncludesFour vials (1mL each) of Lipofectin Transfection Reagent
Product LineLipofectin™
Product TypeTransfection Reagent
Quantity4 mL
Serum CompatibleNo
Shipping ConditionWet Ice
Cell TypeEstablished Cell Lines, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypePlasmid DNA
Transfection TechniqueLipid-based Transfection
Unit SizeEach
Contents & Storage
Each unit contains one or multiple vials (1 ml) of reagent. Store at +4°C. Lipofectin™ Transfection Reagent is guaranteed stable for 6 months when stored properly.
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Guidelines for selecting a transfection reagent
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Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
3180417Certificate of AnalysisJun 24, 202518292011
2797421Certificate of AnalysisAug 06, 202418292011
2669908Certificate of AnalysisMay 13, 202418292037
2621551Certificate of AnalysisNov 02, 202318292037
2735050Certificate of AnalysisSep 07, 202318292011
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
Yes, all of our lipid transfection reagents are stable at room temperature for months.
Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.
We recommend using the Lipofectamine 3000 Reagent for transfection of endothelial cells.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.
For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.
Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.
Citations & References (285)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Characterization of human B creatine kinase gene regulation in the heart in vitro and in vivo.
Journal:The Journal of biological chemistry
PubMed ID:8810319
Isolation and characterization of a novel cDNA encoding a human UDP-glucuronosyltransferase active on C19 steroids.
Journal:The Journal of biological chemistry
PubMed ID:8798464
Transfection techniques for producing recombinant baculoviruses.
Journal:Molecular biotechnology
PubMed ID:9067978
Isolation and cDNA cloning of Ksp-cadherin, a novel kidney-specific member of the cadherin multigene family.
Journal:J Biol Chem
PubMed ID:7615566
Cadherins are recognized as the principal mediators of homotypic cellular recognition and play a demonstrated role in the morphogenic direction of tissue development. We report here the identification of a structurally unique, kidney-specific member of the cadherin multigene family (Ksp-cadherin). cDNA cloning and molecular analysis of the 130-kDa protein confirmed
Temporary ex vivo inhibition of the expression of the human oncogene HER2 (NEU) by a triple helix-forming oligonucleotide.
Journal:Cancer Res
PubMed ID:8564964
A 28-base phosphodiester triple helix-forming oligonucleotide, mostly G and A containing, targeted to a polypurine tract interrupted by a purine-pyrimidine inversion, situated upstream from the TATA box of the promoter of the human HER2 gene, was conceived by computer modeling. The
285 total citations