GlutaMAX™ Supplement
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Try new Pen-Strep antibiotic with GlutaMAX supplement, a stable alternative to L-glutamine that enhances cell health. Learn more >
GlutaMAX™ Supplement
Gibco™

GlutaMAX™ Supplement

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Gibco GlutaMAX Supplement is an alternative to L-glutamine, with increased stability that improves cell health. GlutaMAX Supplement is suitable forRead more
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Catalog NumberQuantity
35050061100 mL
3505007920 x 100 mL
Catalog number 35050061
Price (CNY)
807.00
Each
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Quantity:
100 mL
Price (CNY)
807.00
Each
Add to cart
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Gibco GlutaMAX Supplement is an alternative to L-glutamine, with increased stability that improves cell health. GlutaMAX Supplement is suitable for both adherent and suspension culture of mammalian cells, with no adaptation required. GlutaMAX Supplement is offered as a 200 mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl. GlutaMAX Supplement is also included in a variety of media formulations. Compared to L-glutamine, GlutaMAX Supplement:

• Minimizes toxic ammonia build-up
• Improves cell viability and growth
• Remains stable across a wide range of temperatures

Minimizes toxic ammonia build-up
Unlike L-glutamine, GlutaMAX Supplement does not spontaneously break down to form ammonia. Instead, cells cleave the dipeptide bond to release L-glutamine as needed. This system prevents waste build-up and maintains a fresh supply of L-glutamine during long-term culture.

Improves cell viability and growth
Cultures with lower levels of ammonia show improved cell viability. In addition, culture conditions are more controlled due to the consistent concentration of GlutaMAX Supplement that is not possible with L-glutamine.

Remains stable across a wide range of temperatures
GlutaMAX Supplement is room-temperature stable and ready to use. GlutaMAX Supplement retains full functionality for 24 months at room temperature, 2 to 8°C, or -5 to -30°C for convenient storage and handling. GlutaMAX Supplement is even stable at 37°C for a week or more.

Specifications
Cell TypeMammalian Cells
Concentration100X
Culture TypeMammalian Cell Culture
For Use With (Application)Clinical Research, Translational Research
Green FeaturesSustainable packaging
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality430 to 490 mOsm/kg
Product LineGibco™, GlutaMAX™
Quantity100 mL
Shelf Life24 Months
Shipping ConditionRoom Temperature
SourceAnimal Origin-free
ClassificationAnimal Origin-free
FormLiquid
Product TypeGlutaMax
SterilitySterile-filtered
pH5 to 6
Unit SizeEach
Contents & Storage
Storage conditions: 15°C to 30°C
Alternate storage conditions: -5°C to -20°C and 2°C to 8°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3108168Certificate of AnalysisJun 25, 202535050061, 35050038
3224711Certificate of AnalysisJun 22, 202535050061, 35050038
3209647Certificate of AnalysisJun 22, 202535050061, 35050038
3224713Certificate of AnalysisJun 11, 202535050061, 35050038
3168572Certificate of AnalysisMay 29, 202535050061, 35050038
5 results displayed, search above for a specific certificate

Safety Data Sheets

Frequently asked questions (FAQs)

GlutaMAX Supplement (Cat. No. 35050061, 35050079) is highly recommended to reconstitute Geltrex Reduced Growth Factor Basement Membrane Matrix because it offers higher stability than L-glutamine. The L-glutamine in Geltrex matrix comes in the form of the dipeptide L-alanyl-L-glutamine, which degrades slower than L-glutamine in cell culture. While GlutaMAX supplement is highly recommended, it is not required for Geltrex matrix–based culture systems.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

When using Dynamis AGT Medium (Cat. Nos. A2617501, A2617502, A2617503, A2617504) for culturing a CHO cell line other than the glutamine synthetase (GS) gene expression system (GS-CHO expression system), the addition of 2-8 mM L-glutamine or a similar amount of GlutaMAX Supplement (Cat. No. 35050079, 35050061) is recommended.

Note: We recommend that you supplement with glucose as needed so that glucose levels don’t fall below 2g/L in culture (see the product User Guide for more details).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Some cell lines may require an adaptation period, and the culture may lag in growth until it becomes accustomed to GlutaMAX Supplement (Cat. No. 3050061, 35050079). For serum-free media it is best to add L-glutamine along with GlutaMAX supplement. We don’t recommend that you use GlutaMAX supplement with insect cells such as Sf9.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

The formulation and manufacturing process are the same for all three products. The main difference is in the “intended use” statement. CTS GlutaMAX-I Supplement (Cat. No. A1286001) and CTS GlutaMAX Supplement (Cat. No. A4737001) have an intended use statement of “For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals”. GlutaMAX Supplement (Cat. No. 35050061) has an intended use statement of “For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals”. The catalog number to use will depend on the specific application.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (24)

Citations & References
Abstract
A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.
Authors:Bonanini F,Singh M,Yang H,Kurek D,Harms AC,Mardinoglu A,Hankemeier T
Journal:Experimental cell research
PubMed ID:38499143
Directed differentiation of pancreatic δ cells from human pluripotent stem cells.
Authors:Chen L,Wang N,Zhang T,Zhang F,Zhang W,Meng H,Chen J,Liao Z,Xu X,Ma Z,Xu T,Liu H
Journal:Nature communications
PubMed ID:39068220
Dysfunction of pancreatic δ cells contributes to the etiology of diabetes. Despite their important role, human δ cells are scarce, limiting physiological studies and drug discovery targeting δ cells. To date, no directed δ-cell differentiation method has been established. Here, we demonstrate that fibroblast growth factor (FGF) 7 promotes pancreatic ... More
Effects of an indole derivative on cell proliferation, transfection, and alternative splicing in production of lentiviral vectors by transient co-transfection.
Authors:Mier NC,Roper DK
Journal:PloS one
PubMed ID:38833479
Lentiviral vectors derived from human immunodeficiency virus type I are widely used to deliver functional gene copies to mammalian cells for research and gene therapies. Post-transcriptional splicing of lentiviral vector transgene in transduced host and transfected producer cells presents barriers to widespread application of lentiviral vector-based therapies. The present study ... More
Point mutations identified in Lec8 Chinese hamster ovary glycosylation mutants that inactivate both the UDP-galactose and CMP-sialic acid transporters.
Authors:Oelmann S, Stanley P, Gerardy-Schahn R,
Journal:J Biol Chem
PubMed ID:11319223
'Nucleotide-sugar transporters (NSTs) are critical components of glycosylation pathways in eukaryotes. The identification of structural elements that are involved in NST functions provides an important task. Chinese hamster ovary glycosylation mutants defective in nucleotide-sugar transport provide access to inactive transporters that can define such structure/function relationships. In this study, we ... More
The generation of rhythmic activity in dissociated cultures of rat spinal cord.
Authors:Streit J; Tscherter A; Heuschkel MO; Renaud P
Journal:Eur J Neurosci
PubMed ID:11553272
'Locomotion in vertebrates is controlled by central pattern generators in the spinal cord. The roles of specific network architecture and neuronal properties in rhythm generation by such spinal networks are not fully understood. We were able to induce similar patterns of rhythmic activity in dissociated cultures as in slice ... More
24 total citations

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