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Catalog Number | Quantity |
---|---|
35050061 | 100 mL |
35050079 | 20 x 100 mL |
Gibco GlutaMAX Supplement is an alternative to L-glutamine, with increased stability that improves cell health. GlutaMAX Supplement is suitable for both adherent and suspension culture of mammalian cells, with no adaptation required. GlutaMAX Supplement is offered as a 200 mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl. GlutaMAX Supplement is also included in a variety of media formulations. Compared to L-glutamine, GlutaMAX Supplement:
• Minimizes toxic ammonia build-up
• Improves cell viability and growth
• Remains stable across a wide range of temperatures
Minimizes toxic ammonia build-up
Unlike L-glutamine, GlutaMAX Supplement does not spontaneously break down to form ammonia. Instead, cells cleave the dipeptide bond to release L-glutamine as needed. This system prevents waste build-up and maintains a fresh supply of L-glutamine during long-term culture.
Improves cell viability and growth
Cultures with lower levels of ammonia show improved cell viability. In addition, culture conditions are more controlled due to the consistent concentration of GlutaMAX Supplement that is not possible with L-glutamine.
Remains stable across a wide range of temperatures
GlutaMAX Supplement is room-temperature stable and ready to use. GlutaMAX Supplement retains full functionality for 24 months at room temperature, 2 to 8°C, or -5 to -30°C for convenient storage and handling. GlutaMAX Supplement is even stable at 37°C for a week or more.
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GlutaMAX Supplement (Cat. No. 35050061, 35050079) is highly recommended to reconstitute Geltrex Reduced Growth Factor Basement Membrane Matrix because it offers higher stability than L-glutamine. The L-glutamine in Geltrex matrix comes in the form of the dipeptide L-alanyl-L-glutamine, which degrades slower than L-glutamine in cell culture. While GlutaMAX supplement is highly recommended, it is not required for Geltrex matrix–based culture systems.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
When using Dynamis AGT Medium (Cat. Nos. A2617501, A2617502, A2617503, A2617504) for culturing a CHO cell line other than the glutamine synthetase (GS) gene expression system (GS-CHO expression system), the addition of 2-8 mM L-glutamine or a similar amount of GlutaMAX Supplement (Cat. No. 35050079, 35050061) is recommended.
Note: We recommend that you supplement with glucose as needed so that glucose levels don’t fall below 2g/L in culture (see the product User Guide for more details).
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Some cell lines may require an adaptation period, and the culture may lag in growth until it becomes accustomed to GlutaMAX Supplement (Cat. No. 3050061, 35050079). For serum-free media it is best to add L-glutamine along with GlutaMAX supplement. We don’t recommend that you use GlutaMAX supplement with insect cells such as Sf9.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
The formulation and manufacturing process are the same for all three products. The main difference is in the “intended use” statement. CTS GlutaMAX-I Supplement (Cat. No. A1286001) and CTS GlutaMAX Supplement (Cat. No. A4737001) have an intended use statement of “For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals”. GlutaMAX Supplement (Cat. No. 35050061) has an intended use statement of “For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals”. The catalog number to use will depend on the specific application.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
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