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Invitrogen™
ProLong™ Gold Antifade Mountant
ProLong Gold Antifade is specifically designed to help protect fluorescent dyes (i.e. Alexa Fluor) from fading but is not recommendedRead more
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| Catalog Number | Quantity |
|---|---|
| P36930 | 10 mL |
| P10144 | 2 mL |
| P36934 | 5 x 2 mL |
Catalog number P36930
Price (CNY)
1,805.00
Online Exclusive
Ends: 31-Dec-2025
2,431.00Save 626.00 (26%)
Each
In stock
Quantity:
10 mL
Price (CNY)
1,805.00
Online Exclusive
Ends: 31-Dec-2025
2,431.00Save 626.00 (26%)
Each
ProLong Gold Antifade is specifically designed to help protect fluorescent dyes (i.e. Alexa Fluor) from fading but is not recommended with fluorescent proteins (GFP, RFP, mCherry). It comes ready-to-use and stored at room temperature—just apply a drop on the sample, add a coverslip, cure, and image. ProLong Gold Antifade Mountant is a hard-setting liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. ProLong Gold delivers optimal protection without significantly quenching the initial fluorescence signal to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments. It is a curing mounting media with a refractive index (RI) of 1.47 that allows longer-term storage of mounted samples. ProLong Gold Antifade Mountant is offered in a ready-to-use 2 mL dropper bottle, or 10 mL bottle and is stored at room temperature. Just apply a drop on the sample, add a coverslip, cure, and image.
Achieve Excellent Refractive Index Matching Without Acrylic Resins
For high-resolution imaging applications, refractive index matching is critical. ProLong Gold Antifade Mountant will cure to an RI of 1.47 and allows you to avoid using traditional acrylic resins (i.e., fingernail polish), which can damage microscope objectives if they make contact. The refractive index determines how much the path of light is bent, or refracted, when entering a material. In microscopy, refractive index differences between specimen and mounting medium should be minimized and can therefore be reduced by using mountants with a higher RI. Optimizing the RI of your experiment will increase the resolution to provide better quality images. Depending on your sample type, thickness, imaging depth and/or staining reagents (e.g. fluorescent proteins or dyes) and whether you need a curing or non-curing mountant, choosing the right mounting media will impact image resolution and should be strongly considered for optimal experimental design.
ProLong Gold Antifade Mountant is not recommended for mounting samples containing fluorescent proteins such as GFP or TagRFP. For superior antifade protection of fluorescent proteins and fluorescent dyes, ProLong Diamond or ProLong Glass Antifade Mountants are recommended. Please note that ProLong Glass mounting media is recommended for samples > 10 microns and all immersion oil imaging applications. For immediate viewing of a sample, choose our non-curing mountant, SlowFade Gold Antifade Mountant.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionProLong™ Gold Antifade Mountant, 1 x 10 mL
Green FeaturesLess hazardous, Sustainable packaging
Quantity10 mL
Shipping ConditionRoom Temperature
Product LineProLong™
Product TypeAntifade Mountant
Reagent TypeAntifade Solution
Volume (Metric)10 mL
Unit SizeEach
Contents & Storage
Storage at room temperature is recommended but can also be stored frozen (-5 to -30°C). Protect from light.
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Figures
Qdot®-based labeling of mitochondria in HeLa cells imaged using the EVOS® FL Auto imaging system
Cytoskeleton and mitochondria in HeLa cells imaged on EVOS® FL Auto imaging system
Qdot®-based staining of B-catenin in HeLa cells imaged using the EVOS® FL Auto imaging system
Fixed and stained HDFn cells.
CAKI cell mitochondria and cytoskeleton imaged on EVOS® FL Auto imaging system
Qdot®-based labeling of mitochondria in BPAE cells imaged using the EVOS® FL Auto imaging system
Fixed and stained HDFn cells.
Qdot®-based staining of B-catenin in HeLa cells imaged using the EVOS® FL Auto imaging system
Qdot®-based staining of B-catenin in HeLa cells imaged using the EVOS® FL Auto imaging system
Cytoskeleton and mitochondria in HeLa cells imaged on EVOS® FL Auto imaging system
Golgi and actin in HeLa cells imaged on EVOS® FL Auto imaging system
Cytoskeleton and mitochondria in HeLa cells imaged on EVOS® FL Auto imaging system
Amplification of the GFP signal localized in mitochondria of a transfected HeLa cell.
Qdot®-based labeling of mitochondria in BPAE cells imaged using the EVOS® FL Auto imaging system
Cytoskeleton and mitochondria in HeLa cells imaged on EVOS® FL Auto imaging system
Golgi and actin in HeLa cells imaged on EVOS® FL Auto imaging system
Cytoskeleton captured on EVOS® FL Auto imaging system
Jurkat cells. ManLev tetraacetate, ARP and Alexa Fluor® 488 streptavidin.
Qdot®-based labeling of mitochondria in BPAE cells imaged using the EVOS® FL Auto imaging system
Qdot®-based staining of B-catenin in HeLa cells imaged using the EVOS® FL Auto imaging system
Cytoskeleton and mitochondria in HeLa cells imaged on EVOS® FL Auto imaging system
Tile/stitch image of CAKI cell mitochondria and cytoskeleton captured on EVOS® FL Auto imaging system
Fixed, permeabilized, stained, and blocked BPAE cells.
Fixed and stained HDFn cells.
Peroxisomes in Caki cells imaged on EVOS® FL Auto imaging system
Qdot®-based labeling of mitochondria in BPAE cells imaged using the EVOS® FL Auto imaging system
Mitochondria and cytoskeleton imaged on EVOS® FL Auto imaging system
Enhanced resistance to photobleaching demonstrated by ProLong® Gold antifade reagent.
Qdot®-based labeling of mitochondria in BPAE cells imaged using the EVOS® FL Auto imaging system
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Lot #Certificate TypeDateCatalog Number(s)
3192619Certificate of AnalysisMay 18, 2025P36930
3148397Certificate of AnalysisMay 03, 2025P36930
3148295Certificate of AnalysisApr 22, 2025P10144
3148335Certificate of AnalysisApr 22, 2025P36934
3148310Certificate of AnalysisApr 20, 2025P36934
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Safety Data Sheets
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Molecular Probes® Handbook
Frequently asked questions (FAQs)
Our ProLong antifade reagents dispense as a liquid that will solidify upon the evaporation of water. SlowFade antifade reagents remain liquid. If you are going to image right away and then dispose of your sample, you do not need a mountant that hardens, such as the SlowFade reagents. If you wish to archive your slide for more than a day, you will want a mounting medium that hardens (or “cures”). This hardening will limit the off-rates of various dye-conjugated antibodies and provides a better refractive index. Also, there will be a lower diffusion rate of free radicals, limiting photobleaching.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
ProLong Gold Antifade Mountant hardens overnight at room temperature. For short-term storage (a couple of weeks) you do not need to seal the edges of the coverslip, and the sample should be stable. Beyond that time, though, some dye conjugates will have an off-rate into the medium, so cold storage is recommended. Sealing the edges will prevent long-term discoloration (golden color) from developing around the edges of the coverslip as the anti-oxidants oxidize. The edges may be sealed with melted paraffin, VALAP (1:1:1 vaseline, lanolin, paraffin) or epoxy glue. Nail polish is not recommended as various components of nail polish may diffuse into the mountant and quench fluorescent dyes.
Find additional tips, troubleshooting help, and resources within our Cell Imaging Support Center.
If you are going to image right away and then dispose of your sample, you probably want a mountant that does not harden. If you wish to archive your slide for more than a day, you want a mountant that hardens (or "cures"). This hardening will slow or prevent off-rate of your dye or conjugate and often produces a better refractive index. Secondary sealing is usually not necessary. Also there will be lower diffusion of free radicals, thus limiting photobleaching.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Yes. Put the slide in a Coplin jar or beaker filled with warm (37oC) PBS buffer and let it sit, no agitation is required. The hardened ProLong mountant will swell and may slide off or be easily dislodged. If cells are adherent to the coverslip, make certain the coverslip side containing the cell or tissue sample does not land face down in the container or become scratched upon handling. Remove the coverslip, wash a couple of times, and proceed with re-staining and re-mounting in new ProLong mountant.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
You can image before it cures (hardens), and it will still slow photobleaching, but you have to let it cure overnight to get the best refractive index (resolution). There is no need to seal the edges. In fact, if you seal before it cures, it won't cure correctly. If you are archiving the slide for more than a month, though, seal the edges with resin, paraffin or VALAP (1:1:1 vaseline, lanolin, paraffin) after it cures or there may be slight discoloration along the edges over time.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Citations & References (58)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Cell death and autophagy under oxidative stress: roles of poly(ADP-Ribose) polymerases and Ca(2+).
Journal:Mol Cell Biol
PubMed ID:22751932
On the cellular level, oxidative stress may cause various responses, including autophagy and cell death. All of these outcomes involve disturbed Ca(2+) signaling. Here we show that the nuclear enzymes poly(ADP-ribose) polymerase 1 (PARP1) and PARP2 control cytosolic Ca(2+) shifts from extracellular and intracellular sources associated with autophagy or cell
Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1.
Journal:J Biol Chem
PubMed ID:16807233
'The molecular nature of store-operated Ca(2+)-selective channels has remained an enigma, due largely to the continued inability to convincingly demonstrate Ca(2+)-selective store-operated currents resulting from exogenous expression of known genes. Recent findings have implicated two proteins, Stim1 and Orai1, as having essential roles in store-operated Ca(2+) entry across the plasma
Human stem cell delivery for treatment of large segmental bone defects.
Journal:Proc Natl Acad Sci U S A
PubMed ID:20133731
'Local or systemic stem cell delivery has the potential to promote repair of a variety of damaged or degenerated tissues. Although various stem cell sources have been investigated for bone repair, few comparative reports exist, and cellular distribution and viability postimplantation remain key issues. In this study, we quantified the
Pseudogout-associated inflammatory calcium pyrophosphate dihydrate microcrystals induce formation of neutrophil extracellular traps.
Journal:
PubMed ID:23677474
'Pseudogout is an autoinflammatory condition triggered by calcium pyrophosphate dehydrate (CPPD) crystal deposition in the joints. The innate immune system is irritated by and responds to the presence of the crystals with an inflammatory response. The synovial fluid contains activated inflammatory macrophages and neutrophil granulocytes. Several details of crystal-induced macrophage
Androgen induces expression of the multidrug resistance protein gene MRP4 in prostate cancer cells.
Journal:Prostate Cancer Prostatic Dis
PubMed ID:17003774
'Multidrug resistance-associated proteins (MRPs) may mediate multidrug resistance in tumor cells. Using a gene array analysis, we have identified MRP4 as an androgen receptor (AR)-regulated gene. Dihydrotestosterone induced MRP4 expression in both androgen-dependent and -independent LNCaP cells, whereas there was little detectable expression in PC-3 or normal prostate epithelial cells.
58 total citations