Opti-MEM™ I Reduced Serum Medium, GlutaMAX™ Supplement
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Opti-MEM™ I Reduced Serum Medium, GlutaMAX™ Supplement
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Zitierungen und Referenzen (11)
Gibco™

Opti-MEM™ I Reduced Serum Medium, GlutaMAX™ Supplement

Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine SerumWeitere Informationen
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KatalognummerMenge
51985034Promo Image500 mL
Katalognummer 51985034
Preis (EUR)
115,00
500 mL
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500 mL
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Preis (EUR)
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500 mL
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Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM™ I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM™ I modifications for a range of cell culture applications.


This Opti-MEM™ I is modified as follows:
With
• GlutaMAX™
• Phenol Red


The complete formulation is confidential. For more information, please contact Technical Services.

Using Opti-MEM™ I Medium
Opti-MEM™ I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM™ I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Für die Verwendung in der Forschung oder die Weiterverarbeitung. Nicht zur Diagnosestellung oder direkten Anwendung bei Menschen oder Tieren geeignet.
Specifications
ZelllinieSP2, AE-1, CHO, BHK-21 und HEK
ZelltypPrimäre Fibroblasten
Konzentration1X
Fertigungsqualität, HerstellungsqualitätcGMP-compliant under the ISO 13485 standard
ProduktlinieGibco™, GlutaMAX™, Opti-MEM™
ProdukttypOpti-MEM I
Menge500 mL
Haltbarkeit18 Monate ab Herstellungsdatum
VersandbedingungRaumtemperatur
KlassifikationTierischen Ursprungs
FormFlüssig
SterilitätSteril gefiltert
Mit AdditivenGlutaMAX, Phenolrot
Unit Size500 mL
Inhalt und Lagerung
Lagerbedingungen: 2 bis 8 °C. Vor Licht schützen.
Versandbedingungen: Haltbarkeit bei
Raumtemperatur: 18 Monate ab Herstellungsdatum
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Häufig gestellte Fragen (FAQ)

Is there any other difference between Opti-MEM I Reduced Serum Medium and Opti-MEM I Reduced Serum Medium, no phenol red besides the presence/absence of phenol red?

The formulation of Opti-MEM I Reduced Serum Medium and that of Opti-MEM I Reduced Serum Medium, no phenol red is the same except for the presence of phenol red in the former medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Where can I find the osmolality for my lot of Opti-MEM I Reduced Serum Medium?

We do provide osmolality information on the certificate of analysis. All lots of Opti-MEM I Reduced Serum Medium will meet the osmolality specification of ≥280 to ≤320 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and freeze Opti-MEM I Reduced Serum Medium to conserve it longer? If so, for how long can it be stored?

Sorry, we don't have data to show that frozen Opti-MEM I Reduced Serum Medium has a longer shelf life. Actually, we recommend storing this medium at 2-8 degrees C in the dark. We do not recommend freezing the medium because it may precipitate.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I noticed that Opti-MEM I Reduced Serum Medium does not contain any serum. Why do you then refer to it as "Reduced Serum Medium"?

Opti-MEM I Reduced Serum Medium is a serum-free medium. The reason why it is called “reduced-serum medium” is because you only need to supplement this medium with 1-5% FBS instead of the standard 10% FBS.

Opti-MEM I Reduced Serum Medium can be used as is (without serum) for transfection of cells. However, for long-term cell culture, you need to add serum to the medium to keep cells healthy.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

View all Opti-MEM™ I Reduced Serum Medium, GlutaMAX™ Supplement FAQs

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Chargen-Nr.Certificate TypeDateCatalog Number(s)
3245637Certificate of Analysis06. Juli 202551985034, 51985026
3245092Certificate of Analysis29. Juni 202551985034, 51985026
3199302Certificate of Analysis28. Juni 202551985034, 51985026
3224730Certificate of Analysis06. Juni 202551985034, 51985026
3156244Certificate of Analysis03. Apr. 202551985034, 51985026
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Zitierungen und Referenzen (11)

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Zitierungen und Referenzen
Abstract
Integrin-linked kinase regulates inducible nitric oxide synthase and cyclooxygenase-2 expression in an NF-kappa B-dependent manner.
Authors: Tan Clara; Mui Alice; Dedhar Shoukat;
Journal:J Biol Chem
PubMed ID:11724787
'Nitric oxide (NO) and prostaglandins are produced as a result of the stimulation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2, respectively, in response to cytokines or lipopolysaccharide (LPS). We demonstrate that the activity of integrin-linked kinase (ILK) is stimulated by LPS activation in J774 macrophages. Inhibition of ILK activity ... More
Doxorubicin induces apoptosis and CD95 gene expression in human primary endothelial cells through a p53-dependent mechanism.
Authors: Lorenzo Elisa; Ruiz-Ruiz Carmen; Quesada Antonio Jesús; Hernández Gabriela; Rodríguez Antonio; López-Rivas Abelardo; Redondo Juan Miguel;
Journal:J Biol Chem
PubMed ID:11779855
'Regulation of the homeostasis of vascular endothelium is critical for the processes of vascular remodeling and angiogenesis under physiological and pathological conditions. Here we show that doxorubicin (Dox), a drug used in antitumor therapy, triggered a marked accumulation of p53 and induced CD95 gene expression and apoptosis in proliferating human ... More
Structural and energetic characteristics of the heparin-binding site in antithrombotic protein C.
Authors:Friedrich U, Blom AM, Dahlbäck B, Villoutreix BO,
Journal:J Biol Chem
PubMed ID:11316800
'Human activated protein C (APC) is a key component of a natural anticoagulant system that regulates blood coagulation. In vivo, the catalytic activity of APC is regulated by two serpins, alpha1-antitrypsin and the protein C inhibitor (PCI), the inhibition by the latter being stimulated by heparin. We have identified a ... More
Phospholipase C-gamma is required for agonist-induced Ca2+ entry.
Authors: Patterson Randen L; van Rossum Damian B; Ford Diana L; Hurt Kenneth J; Bae Sun Sik; Suh Pann Ghill; Kurosaki Tomohiro; Snyder Solomon H; Gill Donald L;
Journal:Cell
PubMed ID:12437926
'We report here that PLC-gamma isoforms are required for agonist-induced Ca2+ entry (ACE). Overexpressed wild-type PLC-gamma1 or a lipase-inactive mutant PLC-gamma1 each augmented ACE in PC12 cells, while a deletion mutant lacking the region containing the SH3 domain of PLC-gamma1 was ineffective. RNA interference to deplete either PLC-gamma1 or PLC-gamma2 ... More
Hydrogen peroxide stimulates c-Src-mediated big mitogen-activated protein kinase 1 (BMK1) and the MEF2C signaling pathway in PC12 cells: potential role in cell survival following oxidative insults.
Authors: Suzaki Yuki; Yoshizumi Masanori; Kagami Shoji; Koyama A Hajime; Taketani Yutaka; Houchi Hitoshi; Tsuchiya Koichiro; Takeda Eiji; Tamaki Toshiaki;
Journal:J Biol Chem
PubMed ID:11782488
'Reactive oxygen species, generated by reduction-oxidation (redox) reactions, have been recognized as one of the major mediators of ischemia and reperfusion injury in the brain. Reactive oxygen species-induced cerebral events are attributable, in part, to the change in intracellular signaling molecules including mitogen-activated protein (MAP) kinases. Big MAP kinase 1 ... More
11 total citations

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