DAPI and Hoechst Nucleic Acid Stains
DAPI and Hoechst Nucleic Acid Stains
Invitrogen™

DAPI and Hoechst Nucleic Acid Stains

DAPI and Hoechst are classic popular nuclear counterstains for use in all fluorescent cell and tissue techniques. The blue fluorescence is vivid contrast to green, yellow, orange, red, far red and NIR fluorescent probes and labels.
Promo ImageSonderaktionen
Werbeaktionscode:RIRP25
Save on Imaging Essentials to Create Your Next Masterpiece
14% off your purchase of $499 or more on Invitrogen cell stains and mounting products Weitere Informationen
Have Questions?
Ansicht ändernbuttonViewtableView
KatalognummerMengeProdukttypFarbstofftyp
H3570Promo Image10 mlNukleinsäurefärbemittelHoechst 33342
62248Promo Image1 mlDAPIZellundurchlässig
D21490Promo Image10 mgDAPIZellundurchlässig
D3571Promo Image10 mgDAPIZellundurchlässig
D1306Promo Image10 mgDAPIZellundurchlässig
H3569Promo Image10 mlNukleinsäurefärbemittelHoechst 33258
H1398Promo Image100 mgNukleinsäurefärbemittelHoechst 33258
H21491Promo Image100 mgNukleinsäurefärbemittelHoechst 33258
62249Promo Image5 mlNukleinsäurefärbemittelMembrangängig
H1399Promo Image100 mg, 100 mgNukleinsäurefärbemittelMembrangängig
H21492Promo Image100 mgNukleinsäurefärbemittelHoechst 33342
Katalognummer H3570
Preis (EUR)
207,65
Online Exclusive
220,00
Ersparnis 12,35 (6%)
Each
Vorrätig
Zum Warenkorb hinzufügen
Menge:
10 ml
Produkttyp:
Nukleinsäurefärbemittel
Farbstofftyp:
Hoechst 33342
Recurring order eligible. Learn more »
Preis (EUR)
207,65
Online Exclusive
220,00
Ersparnis 12,35 (6%)
Each
Zum Warenkorb hinzufügen
Ask our AI about this Product

DAPI is a fluorescent dye commonly used in molecular biology and cell biology research. DAPI is known for its ability to bind to DNA, specifically to the minor groove of double-stranded DNA. This dye emits blue fluorescence when excited by ultraviolet light, allowing researchers to visualize and stain DNA in various biological samples.

Hoechst dyes are DNA-specific fluorescent stains that bind to the minor groove of double-stranded DNA. They emit blue fluorescence when excited by ultraviolet light. These dyes are widely used for staining DNA in various biological samples.

The FluoroPure™-grade of Hoechst and DAPI powders are manufactured using stringent purification processes to minimize impurities that could interfere with fluorescence imaging. This ensures that the DAPI and Hoechst provides reliable and consistent results with minimal background noise. The >98% purity of FluoroPure grade reagents make them particularly suitable for demanding applications such as super-resolution microscopy and live-cell imaging, where precise and accurate visualization of DNA is essential.

DAPI (4',6-Diamidino-2-Phenylindole):

DAPI 4',6-diamidino-2-phenylindole is a blue fluorescent probe that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA, where its fluorescence is approximately 20-fold greater than in the non-bound state. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm and efficient contrasting in IHC staining and spatial transcriptomics and proteomics tissue imaging. DAPI is a classic nuclear counterstain for immunofluorescence microscopy, as well as an important component of high-content screening methods requiring cell-based quantitation of DNA content and segmentation (ex/em 341/452 nm).

DAPI as a dilactate powder is more water soluble than the dihydrochloride salt, and therefore a better choice for preparing stock solutions in water.

To make a 5 mg/mL DAPI stock solution (14.3 mM for the dihydrochloride or 10.9 mM for the dilactate), dissolve the contents of one vial (10 mg) in 2 mL of deionized water (dH2O) or dimethylformamide (DMF). The less water-soluble DAPI dihydrochloride may take some time to completely dissolve in water and sonication may be necessary.

Note: Neither of these DAPI derivatives is very soluble in PBS for Stock Solution.

Dilute the DAPI Stock Solution 1:5000 (or 1:1000 for the 1 mg/mL DAPI 62248) in ultrapure water or PBS (1 μg/mL DAPI). Filter the working solution to remove dye aggregates that can result in punctate signal.

Hoechst 33342, Trihydrochloride, Trihydrate and Hoechst 33258, Pentahydrate (bis-Benzimide):

Our Hoechst dyes are renowned fluorophores widely used for DNA staining in both living and fixed cells. With their exceptional affinity and specificity towards DNA, these dyes serve as excellent targeting agents that can be conjugated to various molecules, allowing them to be tethered to DNA.

The Hoechst dyes typically include Hoechst 33258, Hoechst 33342, and Hoechst 34580. Developed by the German company Hoechst AG in the early 1970s, these bisbenzimide dyes have been widely used for DNA staining ever since.

Excited by UV light, Hoechst dyes emit a broad spectrum of blue light. Upon binding to DNA, their fluorescence increases approximately 30-fold, ensuring a strong signal-to-noise ratio. This fluorescence enhancement is the result of suppressed rotational relaxation and hydration reduction upon DNA binding (ex/em 360/460 nm).

Hoechst dyes are non-intercalating, binding specifically to the minor groove of DNA at A–T-rich regions. This unique binding mechanism allows for precise and reliable DNA staining.

Whether you're working with living or fixed cells, our Hoechst dyes are compatible with immunohistochemistry applications. Additionally, the binding of Hoechst 33342 to DNA induces minimal cytotoxicity, ensuring the viability of your cells.

While all three Hoechst dyes have similar applications, they do possess slight differences in their properties. Hoechst 33342, for example, is significantly more cell-permeable due to the addition of a lipophilic ethyl group, making it the preferred choice for living cell staining. On the other hand, Hoechst 34580, with its dimethylamine group instead of the phenol, exhibits a shifted emission maximum at 490 nm, compared to the 461 nm of Hoechst 33258 and Hoechst 33342.

Hoechst 33258, Pentahydrate is also known as bis-Benzimide.

Application of DAPI and Hoechst Fluorescent Dyes:

  • DNA Staining—commonly used for DNA staining in fluorescence microscopy and flow cytometry. It binds to the minor groove of double-stranded DNA and emits blue fluorescence when excited by ultraviolet (UV) light. This allows researchers to visualize and quantify DNA in cells, tissues, and nuclei.
  • Cell Cycle Analysis—widely used to analyze the cell cycle. By staining cells with this dye and using flow cytometry or fluorescence microscopy, researchers can identify and quantify different phases of the cell cycle, such as G1, S, and G2/M phases. This analysis provides insights into cell proliferation, cell cycle progression, and cell cycle abnormalities.
  • Nuclear Morphology and Chromatin Organization—study nuclear morphology and chromatin organization. By visualizing the DNA within the nucleus, researchers can investigate changes in nuclear size, shape, and chromatin condensation, which are associated with various cellular processes and conditions.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications
FarbeBlau
Konzentration10 mg/mL
NachweisverfahrenFluoreszent
FarbstofftypHoechst 33342
Emission461 nm
Anregungswellenlängenbereich350 nm
Zur Verwendung mit (Anwendung)Fluoreszenzmarkierung
Zur Verwendung mit (Geräte)Fluoreszenzmikroskop
FormFlüssig
ProduktlinieHoechst
Menge10 ml
VersandbedingungRaumtemperatur
MarkertypFluorescent Dye
ProdukttypNukleinsäurefärbemittel
SubCellular LocalizationZellkern, Nukleinsäuren, Nucleus
Unit SizeEach
Inhalt und Lagerung
Im Kühlschrank bei 2 °C bis 8 °C aufbewahren und vor Licht schützen.
Have questions about this product? Ask our AI assisted search.
Is DAPI a good live-cell nuclear label?
I need a total cell stain, similar to HCS CellMask Blue stain, to label cytoplasm and nuclei in live cells. What do you recommend?
I have a mix of live bacteria and platelet cells, and I need to be able to separate out the bacteria. Do you have a suggestion?
I want to label the nuclei of live cells and track them over time. Can I use DAPI for this?
DAPI and Hoechst dyes are quite similar to each other. Why would I choose one over the other?
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Abbildungen

Fluoreszenzspektren

Fluorescence spectra

Customers who viewed this item also viewed



Dokumente und Downloads

Zertifikate

Chargen-Nr.Certificate TypeDateCatalog Number(s)
3179517Certificate of Analysis29. Juni 2025H3570
3192604Certificate of Analysis20. Juni 2025H1399
3154587Certificate of Analysis17. Juni 2025H21492
3192334Certificate of Analysis18. Mai 202562248
3163300Certificate of Analysis12. Mai 202562248
5 Ergebnisse angezeigt, oben nach einem bestimmten Zertifikat suchen

Sicherheitsdatenblätter

Häufig gestellte Fragen (FAQ)

DAPI is considered a semi-permeant/impermeant nucleic acid stain. Staining of nucleic is dependent upon the cell line in its performance. Some cell lines will label with DAPI, others not at all, and others label inconsistenly. Instead, we recommend using either Hoechst 33342 or Hoechst 33258, which have the same wavelength and binding mode as DAPI (at the A-T minor groove) but are readily cell-permeant.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

You can use a combination of two dyes with overlapping blue emission. For cytoplasm, you can label the cell with CellTracker Blue CMAC. This can be combined with Hoechst 33342 for nuclei. Both dyes are imaged using a standard DAPI filter set.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

We do not recommend doing this. DAPI is considered to be a semi-permeant/impermeant nucleic acid stain. DAPI staining of live cells may be inconsistent. It is best used as a counterstain for fixed samples. Other cell permeable nucleic acid stains, such as Hoechst or the SYTO dyes may affect cellular function.

For mammalian cells, we recommend using the CellLight Nucleus transduction reagents, available in CFP, GFP and RFP. With these reagents, the cells are transduced overnight in a single labeling step and the next day the nuclei will fluoresce. The label may be retained for 3-5 days and should not affect cell function. Cytoplasmic cell tracking dyes such as the CellTracker dyes may also be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Platelet cells don't have DNA, while bacteria do. Therefore, a cell-permeant, DNA-selective dye would preferentially stain the bacteria with limited staining of the platelets. We recommend using Hoechst 33342 dye.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

DAPI is a very common blue-fluorescent dye for nuclear counterstaining and gives very bright labeling on nuclei in fixed and permeabilized cells and tissues. However, it is considered to be a semi-permeant to impermeant stain and provides inconsistent staining of live cells. Hoechst 33342 dye is cell-permeant and stains with the same binding mechanism and fluorescent color; it is preferred for live-cell imaging and is just as good as DAPI for fixed cell labeling.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Zitierungen und Referenzen (2321)

Zitierungen und Referenzen
Abstract
Hematopoietic potential of stem cells isolated from murine skeletal muscle.
Authors:Jackson KA,Mi T,Goodell MA
Journal:Proceedings of the National Academy of Sciences of the United States of America
PubMed ID:10588731
We have discovered that cells derived from the skeletal muscle of adult mice contain a remarkable capacity for hematopoietic differentiation. Cells prepared from muscle by enzymatic digestion and 5-day in vitro culture were harvested, and 18 × 10(3) cells were introduced into each of six lethally irradiated recipients together with ... More
p53 mediates nontranscriptional cell death in dopaminergic cells in response to proteasome inhibition.
Authors:Nair VD,McNaught KS,González-Maeso J,Sealfon SC,Olanow CW
Journal:The Journal of biological chemistry
PubMed ID:17060322
A natural ErbB4 isoform that does not activate phosphoinositide 3-kinase mediates proliferation but not survival or chemotaxis.
Authors:Kainulainen V,Sundvall M,Määttä JA,Santiestevan E,Klagsbrun M,Elenius K
Journal:The Journal of biological chemistry
PubMed ID:10722704
The organization of acromiodeltoid and spinodeltoid motor nuclei in rat spinal cord.
Authors:Choi JY,Hoover JE
Journal:Brain research
PubMed ID:8949938
The p42/p44 MAP kinase pathway prevents apoptosis induced by anchorage and serum removal.
Authors:Le Gall M,Chambard JC,Breittmayer JP,Grall D,Pouysségur J,Van Obberghen-Schilling E
Journal:Molecular biology of the cell
PubMed ID:10712523
Anchorage removal like growth factor removal induces apoptosis. In the present study we have characterized signaling pathways that can prevent this cell death using a highly growth factor– and anchorage-dependent line of lung fibroblasts (CCL39). After anchorage removal from exponentially growing cells, annexin V-FITC labeling can be detected after 8 ... More
2321 total citations

Geben Sie die Katalognummer, den Namen oder Link frei.