McCoy's 5A (Modified) Medium, HEPES
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Gibco™

McCoy's 5A (Modified) Medium, HEPES

McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cellRead more
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Catalog NumberQuantity
22330021
also known as 22330-021
500 mL
22330070
also known as 22330-070
10 x 500 mL
Catalog number 22330021
also known as 22330-021
Price (EUR)
63,00
Each
Estimated availability date 21-Jul-2025
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Quantity:
500 mL
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Price (EUR)
63,00
Each
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McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.

This McCoy's 5A is modified as follows:

With: High Glucose, L-glutamine, Bacto-peptone, Phenol Red, HEPES

Without: Sodium Pyruvate

The complete formulation is available.

Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator.

Gibco™ McCoy's 5A (modified) requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). Gibco™ McCoy's 5A (modified) uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Specifications
Cell TypeMammalian Cells
ClassificationAnimal Origin-free
Concentration1 X
FormLiquid
Product TypeMcCoy's 5A Medium
Shelf Life12 Months
SterilitySterile-filtered
With AdditivesHigh Glucose, Glutamine, HEPES, Phenol Red, Sodium Bicarbonate
Without AdditivesNo Sodium Pyruvate
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Quantity500 mL
Shipping ConditionRoom Temperature
Osmolality285 - 325 mOsm/kg
pH7 to 7.4
Culture EnvironmentCO2
Culture TypeMammalian Cell Culture
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
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Lot #Certificate TypeDateCatalog Number(s)
3150495Certificate of AnalysisMar 29, 202522330021
2957327Certificate of AnalysisOct 18, 202422330021
2851953Certificate of AnalysisApr 19, 202422330021
2773597Certificate of AnalysisDec 16, 202322330021
2715492Certificate of AnalysisAug 07, 202322330021
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