Fetal Bovine Serum, mesenchymal stem cell-qualified, USDA-approved regions
Fetal Bovine Serum, mesenchymal stem cell-qualified, USDA-approved regions
Gibco™

Fetal Bovine Serum, mesenchymal stem cell-qualified, USDA-approved regions

Gibco™ fetal bovine sera is specially tested for the ability to support the expansion and clonal enumeration (colony forming unit fibroblast assay) of mesenchymal stem cells.
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Catalog NumberQuantity
12662029Promo Image500 mL
Catalog number 12662029
Price (EUR)
980,00
Each
In stock
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Quantity:
500 mL
Recurring order eligible. Learn more »
Price (EUR)
980,00
Each
Add to cart
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Gibco™ fetal bovine sera offers excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

  • Sera Category: Specialty
  • Origin: Countries meeting USDA importation requirements
  • Endotoxin level: ≤50 EU/mL (levels routinely ≤10 EU/mL)
  • Hemoglobin level: ≤25 mg/dL
  • Storage: Store at -5°C to -20°C.

Specially tested for the ability to support the expansion and clonal enumeration (colony forming unit fibroblast assay) of mesenchymal stem cells.

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Specifications
Endotoxin Concentration<50 EU/mL
Hemoglobin Concentration≤25 mg/dL
Purity or Quality GradeMS Cell-Qualified
Shipping ConditionFrozen
SpeciesCattle/Bovine
AgeFetal
Country of OriginCountries meeting USDA importation requirements
FormLiquid
FormatBottle
Product TypeFetal Bovine Serum
Quantity500 mL
Serum TreatmentStandard (Sterile-filtered)
SterilitySterile
Unit SizeEach
Contents & Storage
Storage conditions: ≤-10°C
Shipping conditions: Frozen
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
2100184Certificate of AnalysisJan 29, 202512662029, 12662002, 12662011
2691313RPCertificate of AnalysisJan 29, 202512662029, 12662002, 12662011
2064539Certificate of AnalysisJan 29, 202512662029, 12662002, 12662011
2064554Certificate of AnalysisJan 29, 202512662029, 12662002, 12662011
2249603RPCertificate of AnalysisJan 29, 202512662029, 12662002, 12662011
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Safety Data Sheets

Frequently asked questions (FAQs)

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

You can test each new lot to be sure that it works for your specific applications. Alternatively, our serum sales team can determine the most consistent, highest-performing serum lot available using the Gibco iMatch Sera Lot Matching Tool.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Yes, after the overnight thaw, you can aliquot the serum into smaller, single-use aliquots and refreeze it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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