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StemPro™ Osteogenesis Differentiation Kit
StemPro™ Osteogenesis Differentiation Kit
Gibco™

StemPro™ Osteogenesis Differentiation Kit

For Complete Differentiation of Human Mesenchymal Stem Cells into Bone CellsStemPro™ Osteogenesis Differentiation Kit has been developed for the osteogenicRead more
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Catalog NumberQuantity
A10072011 Kit
Catalog number A1007201
Price (USD)
242.65
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264.00
Save 21.35 (8%)
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Quantity:
1 Kit
Recurring order eligible. Learn more »
Price (USD)
242.65
Online Exclusive
264.00
Save 21.35 (8%)
Each
Add to cart
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For Complete Differentiation of Human Mesenchymal Stem Cells into Bone Cells

StemPro™ Osteogenesis Differentiation Kit has been developed for the osteogenic differentiation of mesenchymal stem cells (MSCs) in tissue culture vessels. The kit contains all reagents required for inducing MSCs to be committed to the osteogenesis pathway and generate
immature and mature osteocytes. Using StemPro™ Osteogenesis Differentiation Kit in combination with MesenPRO RS™ Medium or StemPro™ MSC SFM provides a standardized culture workflow solution for MSC isolation, expansion and differentiation into mineralized matrix-producing osteocytes.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeStem Cells (Mesenchymal)
Culture EnvironmentCO2
Endotoxin Level≤50 EU/mL, ≤ 50 EU/ml
FormatLiquid
GlucoseLow Glucose
Glucose Concentration1000 mg/L
GlutamineGlutaMAX™-I
HEPES BufferNo HEPES
Inorganic SaltsSodium Phosphate
Phenol Red IndicatorNo Phenol Red
Purity or Quality GradeResearch Grade
Quantity1 Kit
Research CategoryDifferentiation
Serum LevelStandard Serum
Sodium Bicarbonate BufferSodium Bicarbonate
Sodium Pyruvate AdditiveSodium Pyruvate
SpeciesHuman
SupplementsOsteogenesis Supplements
For Use With (Application)Stem Cell Differentiation
Product LineGibco, StemPro
Product TypeOsteogenesis Differentiation Kit
Unit SizeEach
Contents & Storage
This kit is shipped in two parts with separate storage requirements. These components are not sold individually:
• StemPro™ Osteocyte/Chondrocyte Differentiation Basal Medium (100 ml; store in the dark at 2°C to 8°C)
• StemPro™ Osteogenesis Supplement (10 ml; store in the dark at -5°C to -20°C)

The Basal Medium ships at room temperature, while the Supplement ships on dry ice.
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Frequently asked questions (FAQs)

Growth factors used for MSC maintenance and self-renewal: LIF, FGF-basic, EGF, HGF, PDGF, and Wnt3.

Growth factors or cytokines for MSC differentiation: BMP-2and TGF-β1.

(Arthritis Res Ther 9:204 (2007)

View our Gibco™ MSC research products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

MSCs may be cultured in either serum-containing medium or serum-free medium. The standard culturing conditions are DMEM, low glucose with 10% FBS. MSCs can also be grown in reduced-serum (2%) Gibco MesenPRO RS Medium (Cat. No. 12746-012), or in serum-free Gibco StemPro MSC SFM XenoFree Medium (Cat. No. A10675) and Gibco StemPro MSC SFM (Cat. No. A10332). In general, MSCs grow better under hypoxic conditions (2% O2).

Human MSCs can be identified by flow cytometry, typically displaying CD73+, CD90+, CD105+, CD11b-, and CD45- marker characteristics (Blood 109:4245 (2007)). Human MSC expresses Oct4, Sox2, and Rex-1; these may be verified using RT-PCR (Arthritis Res Ther 9:204 (2007)).

Mesenchymal stem cells (MSCs) are multipotent cells isolated primarily from bone marrow or fat tissues that exhibit the ability to differentiate into bone, cartilage, and fat cells. Under normal cell culture conditions, MSCs isolated from bone marrow are spindle shaped with the unique ability to adhere to uncoated plastic culture dishes (Arthritis Res Ther 9:204 (2007)).

Citations & References (3)

Citations & References
Abstract
Optimization of human mesenchymal stem cell manufacturing: the effects of animal/xeno-free media.
Authors:Oikonomopoulos A, van Deen WK, Manansala AR, Lacey PN, Tomakili TA, Ziman A, Hommes DW,
Journal:
PubMed ID:26564250
'Due to their immunosuppressive properties, mesenchymal stem cells (MSC) have been evaluated for the treatment of immunological diseases. However, the animal-derived growth supplements utilized for MSC manufacturing may lead to clinical complications. Characterization of alternative media formulations is imperative for MSC therapeutic application. Human BMMSC and AdMSC were expanded in ... More
Alginate-Encapsulation for the Improved Hypothermic Preservation of Human Adipose-Derived Stem Cells.
Authors:Swioklo S, Constantinescu A, Connon CJ,
Journal:Stem Cells Transl Med
PubMed ID:26826163
: Despite considerable progress within the cell therapy industry, unmet bioprocessing and logistical challenges associated with the storage and distribution of cells between sites of manufacture and the clinic exist. We examined whether hypothermic (4°C-23°C) preservation of human adipose-derived stem cells could be improved through their encapsulation in 1.2% calcium ... More
Human Synovial MSC Good Manufacturing Practices for Articular Cartilage Regeneration.
Authors:Fernandes TL, Kimura HA, Pinheiro CCG, Shimomura K, Nakamura N, Ferreira JRM, Gomoll AH, Hernandez AJ, Bueno DF,
Journal:Tissue Eng Part C Methods
PubMed ID:30412046
cartilage restoration is a desperately needed bridge for patients with symptomatic cartilage lesions. Chondral lesion is a pathology with high prevalence, reaching as much as 63% of general population and 36% among athletes. Despite Autologous Chondrocyte Implantation (ACI) versatility, it still fails to fully reproduce hyaline articular cartilage characteristics. Mesenchymal ... More
3 total citations

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