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Invitrogen™
LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells
The LIVE/DEAD® Viability/Cytotoxicity Kit is a quick and easy two-color assay to determine viability of cells in a population basedRead more
| Catalog Number | Quantity |
|---|---|
| L3224 | 1 kit |
Catalog number L3224
Price (EUR)
952,00
Each
In stock
Quantity:
1 kit
Price (EUR)
952,00
Each
The LIVE/DEAD® Viability/Cytotoxicity Kit is a quick and easy two-color assay to determine viability of cells in a population based on plasma membrane integrity and esterase activity. The kit can be used in flow cytometry, fluorescence microscopy, and with fluorescence microplate readers.
Advantages Over Alternative Methods Include:
• Faster
• Safer
• More sensitive
• Less expensive
Easily Use with a Wide Variety of Techniques and Cell Types
Ubiquitous intracellular esterase activity and an intact plasma membrane are distinguishing characteristics of live cells. The LIVE/DEAD® Viability/Cytotoxicity Kit quickly discriminates live from dead cells by simultaneously staining with green-fluorescent calcein-AM to indicate intracellular esterase activity and red-fluorescent ethidium homodimer-1 to indicate loss of plasma membrane integrity. It is adaptable to most eukaryotic cells where cytotoxic conditions produce these cellular effects. The assay is useful with a variety of fluorescence-detection methodologies.
Sensitive, Safe, and Efficient
The LIVE/DEAD® Viability/Cytotoxicity Kit is more sensitive than Trypan blue exclusion, a commonly used method for live/dead cell discrimination. The cost-effective LIVE/DEAD® Viability/Cytotoxicity Kit is highly sensitive due to the bright fluorescence of both dyes upon interacting with either live (for calcein-AM) or dead (for ethidium homodimer-1) cells. Background levels are low due to the fact that both dyes are virtually non-fluorescent prior to interacting with cells.
LIVE/DEAD® Assays Available for a Broad Range of Applications
A selection of Invitrogen LIVE/DEAD® Viability Assays is offered for mammalian cells, bacteria, yeast and fungi, as well as Fixable Dead Cell Stain Kits for use in intracellular staining for flow cytometry. All LIVE/DEAD® assays provide quick, positive discrimination between viable and non-viable cells.
Related Link
• Learn More and See the Full Range of LIVE/DEAD® Assay Products.
Advantages Over Alternative Methods Include:
• Faster
• Safer
• More sensitive
• Less expensive
Easily Use with a Wide Variety of Techniques and Cell Types
Ubiquitous intracellular esterase activity and an intact plasma membrane are distinguishing characteristics of live cells. The LIVE/DEAD® Viability/Cytotoxicity Kit quickly discriminates live from dead cells by simultaneously staining with green-fluorescent calcein-AM to indicate intracellular esterase activity and red-fluorescent ethidium homodimer-1 to indicate loss of plasma membrane integrity. It is adaptable to most eukaryotic cells where cytotoxic conditions produce these cellular effects. The assay is useful with a variety of fluorescence-detection methodologies.
Sensitive, Safe, and Efficient
The LIVE/DEAD® Viability/Cytotoxicity Kit is more sensitive than Trypan blue exclusion, a commonly used method for live/dead cell discrimination. The cost-effective LIVE/DEAD® Viability/Cytotoxicity Kit is highly sensitive due to the bright fluorescence of both dyes upon interacting with either live (for calcein-AM) or dead (for ethidium homodimer-1) cells. Background levels are low due to the fact that both dyes are virtually non-fluorescent prior to interacting with cells.
LIVE/DEAD® Assays Available for a Broad Range of Applications
A selection of Invitrogen LIVE/DEAD® Viability Assays is offered for mammalian cells, bacteria, yeast and fungi, as well as Fixable Dead Cell Stain Kits for use in intracellular staining for flow cytometry. All LIVE/DEAD® assays provide quick, positive discrimination between viable and non-viable cells.
Related Link
• Learn More and See the Full Range of LIVE/DEAD® Assay Products.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeMammalian Cells
DescriptionLIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells
Detection MethodFluorescence
Dye TypeOther Label(s) or Dye(s)
FormatTube(s), 96-well plate, Slide(s)
Quantity1 kit
Shipping ConditionRoom Temperature
ColorGreen, Red
Emission517/617
Excitation Wavelength Range494, 528 nm
For Use With (Application)Viability Assay
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Product LineLIVE/DEAD
Product TypeViability/Cytotoxicity Kit
Unit SizeEach
Contents & Storage
Store in freezer at -5°C to -30°C and protect from light.
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Figures
Rat lung cells. LIVE/DEAD® Viability/Cytotoxicity Kit, ethidium homodimer-1 and calcein AM.
SYTOX Deep Red compared to ethidium homodimer-1
Amsacrine drug titration on U2OS cells
LIVE/DEAD® Viability/Cytotoxicity Kit.
Gambogic acid drug titration
Kangaroo rat (PtK2) cells. LIVE/DEAD® Viability/Cytotoxicity Kit.
Figure 2: Live/Dead® cell viability assay showing live cells stained with Calcein-AM (green) and dead cells with EthD-1 (red):
Live and ethanol-killed bovine pulmonary artery epithelial cells stained with the reagents in our LIVE/DEAD® Cell Viability/Cytotoxicity Assay Kit.
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Citations & References (210)
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Citations & References
Abstract
Enzymatic isolation and characterization of single vascular smooth muscle cells from cremasteric arterioles.
Journal:Microcirculation (New York, N.Y. : 1994)
PubMed ID:9110282
OBJECTIVE: The goal of the present study was to develop a method to isolate viable arteriolar muscle cells from single cremasteric arterioles, which retain the contractile and electrophysiological phenotype of the donor microvessels. METHODS: Arterioles were hand-dissected from rat and hamster cremaster muscles and dissociated by incubation in papain and
Enzymatic isolation and characterization of single vascular smooth muscle cells from cremasteric arterioles.
Journal:Microcirculation (New York, N.Y. : 1994)
PubMed ID:8930888
OBJECTIVE: The goal of the present study was to develop a method to isolate enzymatically viable arteriolar muscle cells from single cremasteric arterioles, which retain the contractile and electrophysiological phenotype of the donor microvessels. METHODS: Arterioles were hand-dissected from rat and hamster cremaster muscles and dissociated by incubation in papain
Calcein: a novel marker for lymphocytes which enter lymph nodes.
Journal:Cytometry
PubMed ID:1451604
Previous studies have identified unique cell surface antigens which are associated with the specific binding of lymphocytes to high endothelial venules (HEV). Evidence is presented in this paper which demonstrates that uptake of the fluorescent dye calcein by lymphocytes represents an additional marker for the lymph node homing subpopulation of
Successful storage of peripheral nerve before transplantation using green tea polyphenol: an experimental study in rats.
Journal:Exp Neurol
PubMed ID:14769360
Green tea polyphenol is known to act as a buffer, reducing biological responses to oxidative stress. Several effects of polyphenol have been reported, such as protection of tissue from ischemia, antineoplasmic and anti-inflammatory effects, and suppression of arteriosclerosis. In this study, we investigated whether peripheral nerve segments could be kept
Human stem cell delivery for treatment of large segmental bone defects.
Journal:Proc Natl Acad Sci U S A
PubMed ID:20133731
'Local or systemic stem cell delivery has the potential to promote repair of a variety of damaged or degenerated tissues. Although various stem cell sources have been investigated for bone repair, few comparative reports exist, and cellular distribution and viability postimplantation remain key issues. In this study, we quantified the
210 total citations