ProLong™ Gold Antifade Mountant with DNA Stain DAPI
ProLong™ Gold Antifade Mountant with DNA Stain DAPI
ProLong™ Gold Antifade Mountant with DNA Stain DAPI
ProLong™ Gold Antifade Mountant with DNA Stain DAPI
Invitrogen™

ProLong™ Gold Antifade Mountant with DNA Stain DAPI

Green features
ProLong Gold Antifade Mountant with DNA Stain DAPI come mixed and ready-to-use to make counterstaining more convenient and consistent while protecting fluorescently stained samples.
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
P369411 x 2 mL
P369355 x 2 mL
P369311 x 10 mL
Catalog number P36941
Price (EUR)
74,65
Offre exceptionnelle en ligne
82,50
Save 7,85 (10%)
Each
-
Add to cart
Quantity:
1 x 2 mL
Price (EUR)
74,65
Offre exceptionnelle en ligne
82,50
Save 7,85 (10%)
Each
Add to cart
Ask our AI about this Product
ProLong Gold Antifade Mountant with DNA Stain DAPI is a mounting media that can be directly applied to fluorescently labeled cell or tissue samples on microscope slides. It comes ready-to-use and stored at room temperature—just apply a drop, add a coverslip, cure, and image. ProLong Gold Antifade Mountants come in a 2-mL dropper bottle or 10-mL bottle. It is formulated with the blue DNA stain DAPI to make your nuclear counterstaining more convenient and consistent when preparing your samples for imaging.

ProLong Gold Antifade Mountant with DNA Stain DAPI contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments. It is a curing mounting media that forms an optical path of 1.47 RI and allows longer-term storage of the sample.

Many mountants can quench initial fluorescence signal strength by up to 80%. ProLong Gold Antifade Mountant was developed to minimize this initial signal quenching for the broadest range of colors and dyes and is formulated with the DAPI DNA nuclear stain to simplify your experiments. DAPI stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm, and is detected using a DAPI traditional filter.

ProLong Gold Antifade Mountant is not recommended for mounting samples containing fluorescent proteins such as GFP or TagRFP but can be used with Alexa Fluor dyes of your choice. For superior antifade protection of fluorescent proteins and fluorescent dyes, ProLong Diamond or ProLong Glass Antifade Mountants are recommended. ProLong Glass mountants are recommended for all immersion oil imaging applications. For immediate viewing of a sample, choose our non-curing mountant, SlowFade Gold Antifade Mountant with DAPI.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Green FeaturesLess hazardous, Sustainable packaging
Quantity1 x 2 mL
Product LineProLong™
Product TypeAntifade Mountant
Reagent TypeMounting Solution, Antifade Solution
Volume (Metric)2 mL
Unit SizeEach
Contents & Storage
Store at room temperature.
four-plex-image-COS7-live-cell zoomed
Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

해당 제품을 검색한 고객들이 함께 검색한 제품



Documents & Downloads

Certificates

Lot #Certificate TypeDateCatalog Number(s)
3148301Certificate of AnalysisJun 20, 2025P36935
3137311Certificate of AnalysisApr 13, 2025P36931
3137329Certificate of AnalysisApr 13, 2025P36935
3137363Certificate of AnalysisApr 10, 2025P36935
3077418Certificate of AnalysisMar 20, 2025P36931
5 results displayed, search above for a specific certificate

Safety Data Sheets

Frequently asked questions (FAQs)

They are composed of proprietary antifades and polymers in aqueous buffers.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

No. If the sample cannot be sealed with a coverslip, we recommend the use of SlowFade antifade mountant.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Our ProLong Gold, ProLong Diamond, and ProLong Glass mounting media harden ('cure') by the evaporation of water. Placing the mounted samples in a humid or refrigerated environment would slow down the evaporation process ('curing').

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

We have not tested ProLong, ProLong Gold, ProLong Diamond, or ProLong Glass on DyLight dye-conjugated proteins or with other organic dyes, but it is likely that they will be compatible.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

No. ProLong, ProLong Gold, ProLong Diamond, and ProLong Glass reagents are intended for use on fixed or fixed/permeabilized samples. Only ProLong Live Antifade Reagent (Cat Nos. P36974 and P36975) is intended for use with live cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (31)

Citations & References
Abstract
Super-resolution imaging reveals three-dimensional folding dynamics of the ß-globin locus upon gene activation.
Authors:van de Corput MP, de Boer E, Knoch TA, van Cappellen WA, Quintanilla A, Ferrand L, Grosveld FG,
Journal:J Cell Sci
PubMed ID:22767512
The chromatin architecture is constantly changing because of cellular processes such as proliferation, differentiation and changes in the expression profile during gene activation or silencing. Unravelling the changes that occur in the chromatin structure during these processes has been a topic of interest for many years. It is known that ... More
Resolution of de novo HIV production and trafficking in immature dendritic cells.
Authors:Turville SG, Aravantinou M, Stössel H, Romani N, Robbiani M,
Journal:Nat Methods
PubMed ID:18059278
'The challenge in observing de novo virus production in human immunodeficiency virus (HIV)-infected dendritic cells (DCs) is the lack of resolution between cytosolic immature and endocytic mature HIV gag protein. To track HIV production, we developed an infectious HIV construct bearing a diothiol-resistant tetracysteine motif (dTCM) at the C terminus ... More
Androgen induces expression of the multidrug resistance protein gene MRP4 in prostate cancer cells.
Authors:Cai C, Omwancha J, Hsieh CL, Shemshedini L
Journal:Prostate Cancer Prostatic Dis
PubMed ID:17003774
'Multidrug resistance-associated proteins (MRPs) may mediate multidrug resistance in tumor cells. Using a gene array analysis, we have identified MRP4 as an androgen receptor (AR)-regulated gene. Dihydrotestosterone induced MRP4 expression in both androgen-dependent and -independent LNCaP cells, whereas there was little detectable expression in PC-3 or normal prostate epithelial cells. ... More
H2AX phosphorylation within the G1 phase after UV irradiation depends on nucleotide excision repair and not DNA double-strand breaks.
Authors:Marti TM, Hefner E, Feeney L, Natale V, Cleaver JE
Journal:Proc Natl Acad Sci U S A
PubMed ID:16788066
'The variant histone H2AX is phosphorylated in response to UV irradiation of primary human fibroblasts in a complex fashion that is radically different from that commonly reported after DNA double-strand breaks. H2AX phosphorylation after exposure to ionizing radiation produces foci, which are detectable by immunofluorescence microscopy and have been adopted ... More
AZD2171: a highly potent, orally bioavailable, vascular endothelial growth factor receptor-2 tyrosine kinase inhibitor for the treatment of cancer.
Authors:Wedge SR, Kendrew J, Hennequin LF, Valentine PJ, Barry ST, Brave SR, Smith NR, James NH, Dukes M, Curwen JO, Chester R, Jackson JA, Boffey SJ, Kilburn LL, Barnett S, Richmond GH, Wadsworth PF, Walker M, Bigley AL, Taylor ST, Cooper L, Beck S, Jürgensmeier JM, Ogilvie DJ,
Journal:Cancer Res
PubMed ID:15899831
'Inhibition of vascular endothelial growth factor-A (VEGF) signaling is a promising therapeutic approach that aims to stabilize the progression of solid malignancies by abrogating tumor-induced angiogenesis. This may be accomplished by inhibiting the kinase activity of VEGF receptor-2 (KDR), which has a key role in mediating VEGF-induced responses. The novel ... More
31 total citations

Share catalog number, name or link

1x1 image pixel for data collection