Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Catalog Number | Quantity |
---|---|
P36941 | 1 x 2 mL |
P36935 | 5 x 2 mL |
P36931 | 1 x 10 mL |
ProLong Gold Antifade Mountant with DNA Stain DAPI contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments. It is a curing mounting media that forms an optical path of 1.47 RI and allows longer-term storage of the sample.
Many mountants can quench initial fluorescence signal strength by up to 80%. ProLong Gold Antifade Mountant was developed to minimize this initial signal quenching for the broadest range of colors and dyes and is formulated with the DAPI DNA nuclear stain to simplify your experiments. DAPI stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm, and is detected using a DAPI traditional filter.
ProLong Gold Antifade Mountant is not recommended for mounting samples containing fluorescent proteins such as GFP or TagRFP but can be used with Alexa Fluor dyes of your choice. For superior antifade protection of fluorescent proteins and fluorescent dyes, ProLong Diamond or ProLong Glass Antifade Mountants are recommended. ProLong Glass mountants are recommended for all immersion oil imaging applications. For immediate viewing of a sample, choose our non-curing mountant, SlowFade Gold Antifade Mountant with DAPI.
Finding the right threshold is important when choosing your conjugate. Use our new Degree of Labeling Calculator for optimal labeling.
Explore our DoL Calculator ›
They are composed of proprietary antifades and polymers in aqueous buffers.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
No. If the sample cannot be sealed with a coverslip, we recommend the use of SlowFade antifade mountant.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Our ProLong Gold, ProLong Diamond, and ProLong Glass mounting media harden ('cure') by the evaporation of water. Placing the mounted samples in a humid or refrigerated environment would slow down the evaporation process ('curing').
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
We have not tested ProLong, ProLong Gold, ProLong Diamond, or ProLong Glass on DyLight dye-conjugated proteins or with other organic dyes, but it is likely that they will be compatible.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
No. ProLong, ProLong Gold, ProLong Diamond, and ProLong Glass reagents are intended for use on fixed or fixed/permeabilized samples. Only ProLong Live Antifade Reagent (Cat Nos. P36974 and P36975) is intended for use with live cells.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Share catalog number, name or link