El kit de limpieza de glicanos PNGasa F incluye todos los componentes necesarios para realizar la eliminación enzimática de casiMás información
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Número de catálogo
Cantidad
A39245
1 kit
Número de catálogo A39245
Precio (EUR)
323,00
Each
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Cantidad:
1 kit
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Precio (EUR)
323,00
Each
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El kit de limpieza de glicanos PNGasa F incluye todos los componentes necesarios para realizar la eliminación enzimática de casi todos los N-oligosacáridos vinculados de las glicoproteínas. El kit incluye la enzima PNGasa F, que divide las cadenas N-glicanas en el GlcNAc más interno y los residuos de asparagina de complejos oligosacáridos híbridos de manosa alta.
Las características del kit de limpieza de glicanos PNGasa F incluyen: • ≥95 % de pureza • Enzima recombinante PNGasa F sin endoglucosidasa F1, F2 o F3 • La enzima deja intactos los oligosacáridos del núcleo N-glicano y son adecuados para análisis posteriores • Adecuados para su uso en condiciones nativas o de desnaturalización
Los componentes incluidos en el Kit de limpieza de glicanos PNGasa F son: 30.000 unidades de PNGasa F (500.000 unidades/ml) y tampón PNGasa F 10x de 1 ml
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
FormularioLíquido
Cantidad1 kit
Condiciones de envíoHielo húmedo
Tipo de productoKit de disociación de glicanos PNGasa F.
Unit SizeEach
Contenido y almacenamiento
• 30.000 unidades de enzima PNGasa F, recombinante (500.000 unidades/ml), almacenar a - 20 °C • Tampón PNGasa F 10x de 1 ml, almacenar a - 20 °C
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Preguntas frecuentes
In the PNGase Glycan Cleavage Kit, can I use PNGase F under native or denaturing conditions?
Yes, PNGase F enzyme may be used in both native and denaturing conditions
In the PNGase Glycan Cleavage Kit, how much PNGase F should I use to remove my carbohydrate under native or DTT denaturing conditions?
When the protein is not denatured with SDS, PNGase F has to work harder to reach the cleavage site of the carbohydrate (due to secondary and tertiary protein structures). In these instances, using more enzyme and/or extended incubation times may help. These conditions will need to be determined empirically for your specific protein.
In the PNGase Glycan Cleavage Kit, is PNGase F compatible with downstream analysis such as HPLC and mass spectrometry?
PNGase F enzyme is stored in 50% glycerol. Therefore, HPLC and/or mass spectrometry is not compatible due to the fact that glycerol is not tolerated in instruments used for these applications.
In the PNGase Glycan Cleavage Kit, what are the typical reaction conditions for PNGase F, Recombinant?
Typical reaction conditions are as follows:
Optimal incubation times and enzyme concentrations must be determined empirically for a particular substrate.
1. Combine 1-20 µg of glycoprotein, 1 µL of 10X denaturing buffer (5% SDS, 400 mM DTT) and H2O (if necessary) to make a 10 µL total reaction volume.
2. Denature glycoprotein by heating reaction at 100 degrees C for 10 min.
3. Make a total reaction volume of 20 µL by adding 2 µL PNGaseF 10X Buffer, 1-5 µL PNGaseF enzyme, 2 µL 10% NP-40 and H2O.
4. Incubate reaction at 37 degrees C for 1 hr.
Note: Reactions may be scaled-up linearly to accommodate larger reaction volumes.