Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Detection Method | Gel Electrophoresis |
Format | Kit |
GC-Rich PCR Performance | High |
PCR Method | 1-step RT-PCR |
Polymerase | Platinum SuperFi |
Reaction Speed | 10 min. |
Technique | 1-step RT-PCR |
Optimal Reaction Temperature | 45°C to 60°C |
Reverse Transcriptase | SuperScript IV |
Ribonuclease H Activity | Reduced |
Shipping Condition | Dry Ice |
Final Product Type | PCR Amplified cDNA |
Hot Start | Built-In Hot Start |
No. of Reactions | 25 Reactions |
Reaction Format | Master Mix |
Reagent Type | Reverse Transcription |
Size (Final Product) | Up to 14 kb |
Starting Material | RNA |
Includes | Kit with ezDNase |
Unit Size | Each |
Detection Method | Gel Electrophoresis |
Format | Kit |
GC-Rich PCR Performance | High |
PCR Method | 1-step RT-PCR |
Polymerase | Platinum SuperFi |
Reaction Speed | 10 min. |
Technique | 1-step RT-PCR |
Optimal Reaction Temperature | 45°C to 60°C |
Reverse Transcriptase | SuperScript IV |
Ribonuclease H Activity | Reduced |
Shipping Condition | Dry Ice |
Final Product Type | PCR Amplified cDNA |
Hot Start | Built-In Hot Start |
No. of Reactions | 25 Reactions |
Reaction Format | Master Mix |
Reagent Type | Reverse Transcription |
Size (Final Product) | Up to 14 kb |
Starting Material | RNA |
Includes | Kit only |
Unit Size | Each |
Detection Method | Gel Electrophoresis |
Format | Kit |
GC-Rich PCR Performance | High |
PCR Method | 1-step RT-PCR |
Polymerase | Platinum SuperFi |
Reaction Speed | 10 min. |
Technique | 1-step RT-PCR |
Optimal Reaction Temperature | 45°C to 60°C |
Reverse Transcriptase | SuperScript IV |
Ribonuclease H Activity | Reduced |
Shipping Condition | Dry Ice |
Final Product Type | PCR Amplified cDNA |
Hot Start | Built-In Hot Start |
No. of Reactions | 100 Reactions |
Reaction Format | Master Mix |
Reagent Type | Reverse Transcription |
Size (Final Product) | Up to 14 kb |
Starting Material | RNA |
Includes | Kit only |
Unit Size | Each |
Detection Method | Gel Electrophoresis |
Format | Kit |
GC-Rich PCR Performance | High |
PCR Method | 1-step RT-PCR |
Polymerase | Platinum SuperFi |
Reaction Speed | 10 min. |
Technique | 1-step RT-PCR |
Optimal Reaction Temperature | 45°C to 60°C |
Reverse Transcriptase | SuperScript IV |
Ribonuclease H Activity | Reduced |
Shipping Condition | Dry Ice |
Final Product Type | PCR Amplified cDNA |
Hot Start | Built-In Hot Start |
No. of Reactions | 100 Reactions |
Reaction Format | Master Mix |
Reagent Type | Reverse Transcription |
Size (Final Product) | Up to 14 kb |
Starting Material | RNA |
Includes | Kit with ezDNase |
Unit Size | Each |
Catalog Number | Specifications | Unit Size | Includes | No. of Reactions | Price (EUR) | Availability | Quantity | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
12595025 | Each | Kit with ezDNase | 25 Reactions | 330,65 Online price 414,00 | - | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12594025 | Each | Kit only | 25 Reactions | 329,65 Online price 385,00 | - | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12594100 | Each | Kit only | 100 Reactions | 878,65 Online price 1 094,00 | - | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12595100 | Each | Kit with ezDNase | 100 Reactions | 1 216,00 | - | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
Features of the SuperScript IV One-Step RT-PCR System include:
• Superior performance with sensitivity down to 0.01 pg RNA, target length up to 13.8 kb, and the fastest one-step RT-PCR protocol
• Reliable target detection even with suboptimally pure RNA samples
• Two-phase hot-start activation mechanism for high sensitivity, specificity, and room temperature setup
• Fast and easy gDNA removal for superior accuracy and confidence in results (SuperScript IV One-Step RT-PCR System with ezDNase only)
SuperScript IV RT is a proprietary MMLV RT mutant with increased processivity, thermostability, and inhibitor resistance, as well as reduced RNase H activity, for sensitive and efficient full-length cDNA synthesis. Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. The combination of these two engineered enzymes produces enhanced RT-PCR yields and fidelity, as well as amplification of longer templates compared to other one-step RT-PCR kits.
The inclusion of ezDNase (SuperScript IV One-Step RT-PCR System with ezDNase only) for use upstream of the RT step further enhances the one-step RT-PCR workflow through an extremely simplified genomic DNA removal step that ensures the highest accuracy and confidence in one-step RT-PCR results.
• SuperScript IV RT Mix (12.5 μL)
• 2X Platinum SuperFi RT-PCR Master Mix (625 μL)
• ezDNase Enzyme (25 μL)
• 10X ezDNase Buffer (100 μL)
• Nuclease-free water (1.25 mL)
Store at –20°C.