pHrodo™ Red and Green AM Intracellular pH Indicator Dyes
pHrodo™ Red and Green AM Intracellular pH Indicator Dyes
pHrodo™ Red and Green AM Intracellular pH Indicator Dyes
pHrodo™ Red and Green AM Intracellular pH Indicator Dyes
pHrodo™ Red and Green AM Intracellular pH Indicator Dyes
pHrodo™ Red and Green AM Intracellular pH Indicator Dyes
pHrodo™ Red and Green AM Intracellular pH Indicator Dyes
Invitrogen™

pHrodo™ Red and Green AM Intracellular pH Indicator Dyes

Quantify intracellular pH with pHrodo Red and Green AM intracellular pH indicator dyes, which permeate cell membranes and fluoresce in acidic cytosolic environments. Both the pHrodo Red and Green AM indicator dyes are compatible with HCS, fluorescence microplate, flow cytometry, and HTS platforms.
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P35372
P35373
Catalog number P35372
Price (EUR)
614,00
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Price (EUR)
614,00
Each
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Measure and quantify intracellular pH with pHrodo Red and Green AM indicator dyes, which are fluorogenic probes that traverse the cell membrane and remain within the intracellular space upon cleavage by nonspecific esterases. pHrodo Red and Green AM indicator dyes become increasingly fluorescent as the pH drops and can be used to quantify cytosolic pH in the range of 4–9. These indicator dyes can also be used in multiplex experiments within HCS, flow cytometry, microplate fluorometry, and HCS applications.
pHrodo Red (Cat. No. P35372) and Green (Cat. No. P35373) AM intracellular pH indicators are fluorogenic probes used to measure intracellular pH in live cells. They are weakly fluorescent at neutral pH but become increasingly fluorescent as the pH decreases. These reagents can be used to quantify cellular cytosolic pH in the range of 4–9 with a pKa of ∼6.5 and with excitation/emission spectra of 560/585 nm (pHrodo Red) or 509/533 nm (pHrodo Green).

Both pHrodo Red and pHrodo Green pH indicator dyes are photostable and appropriate for use in multiplexing experiments. pHrodo Red easily multiplexes with a wide variety of blue, green, and far red dyes and reporters such as GFP, Fuo-4, calcein, NucBlue, CellEvent Caspase 3/7 green, Mitosox Green, and Mitotracker Deep Red, among many others. pHrodo Green easily multiplexes with a wide variety of blue, red, and far red dyes reporters such as Mitosox Red, CellEvent Caspase 3/7 Red, NucBlue, RFPs, and Mitotracker Deep Red, among many others.

The pHrodo Red and Green dyes have been modified with AM ester groups, resulting in an uncharged molecule that can permeate cell membranes. Once inside the cell, the lipophilic blocking groups of the dyes are cleaved by nonspecific esterases, resulting in compounds that are retained within the intracellular space. The fluorescence intensity of the probe then becomes an indicator of intracellular pH. Subsequent use of the Intracellular pH Calibration Buffer Kit (Cat. No. P35379) allows this intracellular pH to be quantified.

pHrodo Red and Green AM intracellular pH indicator dyes are compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), flow cytometry, and microplate-based fluorimetry or high throughput screening (HTS). These reagents are also compatible with widefield or confocal fluorescence microscopy, flow cytometry, fluorescence plate readers, and high content instruments.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.
Specifications
DescriptionpHrodo™ Red AM Intracellular pH Indicator
Detection MethodFluorescence
Dye TypeFluorescent Dye-Based
FormLiquid
Quantity50 μL
SolubilityDMSO (Dimethylsulfoxide)
Sub Cellular LocalizationCytoplasm
ColorOrange
EmissionVisible
For Use With (Application)Cell Analysis
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Fluorometer, High Content Analysis Instrument
Product LinepHrodo
Product TypepH Indicator
Unit SizeEach
Contents & Storage
Store at 2–8°C, desiccate, and protect from light.

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live-cell-tracking-with-phrodo-dyes
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Lot #Certificate TypeDateCatalog Number(s)
3183386Certificate of AnalysisJun 29, 2025P35373
3057090Certificate of AnalysisApr 03, 2025P35372
2942290Certificate of AnalysisSep 19, 2024P35373
2980673Certificate of AnalysisSep 04, 2024P35372
2845307Certificate of AnalysisFeb 01, 2024P35373
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Citations & References (23)

Citations & References
Abstract
HIV-1 and morphine regulation of autophagy in microglia: Limited interactions in the context of HIV-1 infection and opioid abuse.
Authors:El-Hage N, Rodriguez M, Dever SM, Masvekar RR, Gewirtz DA, Shacka JJ,
Journal:
PubMed ID:25355898
'Microglia are the predominant resident central nervous system (CNS) cell type productively infected by human immunodeficiency virus (HIV) type-1, and play a key role in the progression of HIV-associated dementia (HAD). Moreover, neural dysfunction and progression to HAD are accelerated in opiate drug abusers. In the present study, we examined ... More
Neuroprotective effects of mGluR II and III activators against staurosporine- and doxorubicin-induced cellular injury in SH-SY5Y cells: New evidence for a mechanism involving inhibition of AIF translocation.
Authors:Jantas D, Greda A, Leskiewicz M, Grygier B, Pilc A, Lason W,
Journal:
PubMed ID:25661514
There are several experimental data sets demonstrating the neuroprotective effects of activation of group II and III metabotropic glutamate receptors (mGluR II/III), however, their effect on neuronal apoptotic processes has yet to be fully recognized. Thus, the comparison of the neuroprotective potency of the mGluR II agonist LY354740, mGluR III ... More
Macrophage Metabolism of Apoptotic Cell-Derived Arginine Promotes Continual Efferocytosis and Resolution of Injury.
Authors:
Journal:Cell Metab
PubMed ID:32004476
Histone chaperone FACT complex coordinates with HIF to mediate an expeditious transcription program to adapt to poorly oxygenated cancers.
Authors:
Journal:Cell Rep
PubMed ID:35108543
Carbonic anhydrase 12 mutation modulates membrane stability and volume regulation of aquaporin 5.
Authors:Hwang S, Kang JY, Kim MJ, Shin DM, Hong JH
Journal:J Enzyme Inhib Med Chem
PubMed ID:30451023
'Patients carrying the carbonic anhydrase12 E143K mutation showed the dry mouth phenotype. The mechanism underlying the modulation of aquaporin 5 and function in the salivary glands by carbonic anhydrase12 remains unknown. In this study, we identified the mislocalised aquaporin 5 in the salivary glands carrying the E143K. The intracellular pH ... More
23 total citations

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