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Catalog Number | Unit Size | Price (JPY) | Availability | |
---|---|---|---|---|
11754250 | 250 reactions | Price: 215,000 | *** | |
11754050 | 50 reactions | Price: 52,000 | *** |
The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis.
The SuperScript VILO cDNA Synthesis Kit can be used to synthesize cDNA from for a wide range of input RNA amounts. This kit provides the high temperature capability of SuperScript III Reverse Transcriptase in an optimized format for generating first-strand cDNA for use in RT–qPCR.
Notes
• For superior first-strand cDNA synthesis in two-step RT-qPCR, try SuperScript IV VILO Master Mix or SuperScript IV VILO Master Mix with ezDNase Enzyme.
• This kit is included with EXPRESS Two-Step RT–qPCR kits. Reagents are provided for 50 or 250 20-μL cDNA synthesis reactions.
• 5X VILO Reaction Mix, 1,000 μL
• 10X SuperScript III Enzyme Blend, 500 μL
Store at –5 to –30°C.