ER-Tracker Blue-White DPX dye is a photostable probe that is selective for the endoplasmic reticulum (ER) in live cells. The cells can be aldehyde-fixed after staining with the dye but significant fluorescence signal will be lost. With excitation at ∼374 nm, the environmentally-sensitive probe exhibits emission between 430 and 640 nm. A long-pass DAPI filter works best.
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Abstract
CD1d degradation in Chlamydia trachomatis-infected epithelial cells is the result of both cellular and chlamydial proteasomal activity.
Authors:Kawana K, Quayle AJ, Ficarra M, Ibana JA, Shen L, Kawana Y, Yang H, Marrero L, Yavagal S, Greene SJ, Zhang YX, Pyles RB, Blumberg RS, Schust DJ
Journal:J Biol Chem
PubMed ID:17215251
'Chlamydia trachomatis is an obligate intracellular pathogen that can persist in the urogenital tract. Mechanisms by which C. trachomatis evades clearance by host innate immune responses are poorly described. CD1d is MHC-like, is expressed by epithelial cells, and can signal innate immune responses by NK and NKT cells. Here we ... More
Microtubules regulate local Ca2+ spiking in secretory epithelial cells.
Authors:Fogarty KE, Kidd JF, Turner A, Skepper JN, Carmichael J, Thorn P
Journal:J Biol Chem
PubMed ID:10801885
'The role of the cytoskeleton in regulating Ca(2+) release has been explored in epithelial cells. Trains of local Ca(2+) spikes were elicited in pancreatic acinar cells by infusion of inositol trisphosphate through a whole cell patch pipette, and the Ca(2+)-dependent Cl(-) current spikes were recorded. The spikes were only transiently ... More
Apoptosis in tumour cells photosensitized with Rose Bengal acetate is induced by multiple organelle photodamage.
Authors:Soldani C, Croce AC, Bottone MG, Fraschini A, Biggiogera M, Bottiroli G, Pellicciari C,
Journal:Histochem Cell Biol
PubMed ID:17849139
'Rose Bengal (RB) is a very efficient photosensitizer which undergoes inactivation of its photophysical and photochemical properties upon addition of a quencher group-i.e. acetate-to the xanthene rings. The resulting RB acetate (RB-Ac) derivative behaves as a fluorogenic substrate: it easily enters the cells where the native photoactive molecule is restored ... More
TLR9 signals after translocating from the ER to CpG DNA in the lysosome.
Authors:Latz E, Schoenemeyer A, Visintin A, Fitzgerald KA, Monks BG, Knetter CF, Lien E, Nilsen NJ, Espevik T, Golenbock DT
Journal:Nat Immunol
PubMed ID:14716310
'Microbial DNA sequences containing unmethylated CpG dinucleotides activate Toll-like receptor 9 (TLR9). We have found that TLR9 is localized to the endoplasmic reticulum (ER) of dendritic cells (DCs) and macrophages. Because there is no precedent for immune receptor signaling in the ER, we investigated how TLR9 is activated. We show ... More
Two-photon fluorescence absorption and emission spectra of dyes relevant for cell imaging.
Authors:Bestvater F, Spiess E, Stobrawa G, Hacker M, Feurer T, Porwol T, Berchner-Pfannschmidt U, Wotzlaw C, Acker H
Journal:J Microsc
PubMed ID:12423261
'Two-photon absorption and emission spectra for fluorophores relevant in cell imaging were measured using a 45 fs Ti:sapphire laser, a continuously tuneable optical parametric amplifier for the excitation range 580-1150 nm and an optical multichannel analyser. The measurements included DNA stains, fluorescent dyes coupled to antibodies as well as organelle ... More