Phusion™ Plus PCR Master Mixes
Phusion™ Plus PCR Master Mixes
Thermo Scientific™

Phusion™ Plus PCR Master Mixes

Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.
Catalog NumberColorNo. of Reactions
F631SColorless100 Reactions
F632LGreen500 Reactions
F632SGreen100 Reactions
F632XLGreen2000 Reactions
F631LColorless500 Reactions
F631XLColorless2000 Reactions
Catalog number F631S
Price (JPY)
32,000
Each
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Color:
Colorless
No. of Reactions:
100 Reactions
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Thermo Scientific Phusion Plus Green PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs. The master mix format simplifies reaction setup with minimal pipetting steps and retains all features of Phusion Plus DNA Polymerase. Additionally, two tracking dyes and a density reagent are pre-added to the master mix for direct loading of PCR products on gels.

Features of Phusion™ Plus PCR Master Mix include:

  • High fidelity—provides >100X higher sequence accuracy than Taq polymerase
  • Convenient setup—enables a universal annealing temperature of 60°C in PCR
  • Enhanced specificity—hot-start mechanism reduces non-specific amplification and primer degradation
  • Benchtop stability—automation enabled due to 24-hour stability of assembled reactions
  • High inhibitor tolerance—works with DNA of suboptimal purity

Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing. It is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain, enabling Phusion Plus PCR Master Mix to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance. Its novel reaction buffer includes proprietary additives that stabilize primer/template duplexes during the annealing step at 60°C. This unique buffer formulation increases PCR specificity without the need to optimize annealing temperature for each primer pair, helping save time and avoid mistakes in PCR runs. Furthermore, with Phusion Plus PCR Master Mix and its universal annealing feature, different PCR reactions can be cycled together using the same protocol.

Phusion Plus PCR Master Mix is inactive at ambient temperatures due to its hot-start mechanism mediated by Affibody molecules, allowing setup and storage of assembled PCR reactions at room temperature. Enzyme activity is fully restored after the initial denaturation step.

Applications

  • High-fidelity PCR
  • Cloning
  • Template generation for sequencing
  • Amplification of difficult (GC-rich) templates
  • Long-range PCR (up to 20 kb)
  • Mutagenesis
  • High-throughput PCR
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorColorless
Concentration2X
Fidelity (vs. Taq)>100X
Hot StartBuilt-In Hot Start
No. of Reactions100 Reactions
OverhangBlunt
PolymerasePhusion Plus DNA Polymerase
Quantity100 x 50 μL reactions
Reaction FormatReady to use master mix
Shipping ConditionDry Ice
Size (Final Product)20 kb or less
For Use With (Application)Hot-start PCR, High-fidelity PCR
GC-Rich PCR PerformanceHigh
Reaction Speed15 to 30 sec./kb
Unit SizeEach
Contents & Storage
• 2X Phusion Plus PCR Master Mix (2 x 1.25 mL)
• 5X Phusion GC Enhancer (1.25 mL)
• Nuclease-free water (2 x 1.25 mL)

Store at –15°C to –25°C
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3273267Certificate of AnalysisJul 03, 2025F632S
3273252Certificate of AnalysisJul 03, 2025F632S
3262777Certificate of AnalysisJun 17, 2025F632L
3258884Certificate of AnalysisJun 11, 2025F631L
3253021Certificate of AnalysisJun 03, 2025F632L
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Frequently asked questions (FAQs)

Reaction setup with Phusion Plus DNA Polymerase or Phusion Plus PCR Master Mix can be done either on ice or at room temperature.

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