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EXPRESS One-Step Superscript™ qRT-PCR Kit, universal
EXPRESS One-Step SuperScript qRT-PCR kits incorporate proven Invitrogen enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for詳細を見る
製品番号（カタログ番号） 1178101K
価格（JPY）お問い合わせください ›
449,300
Each
反応数:
反応2500回分
EXPRESS One-Step SuperScript qRT-PCR kits incorporate proven Invitrogen enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for your probe-based qPCR experiments. This fast, one-step mix is designed to work with a variety of instruments’ default protocols, including Applied Biosystems and non-Applied Biosystem (see universal version) qPCR platforms.
Features of the EXPRESS One-Step Superscript qRT-PCR Kit include:
• Fast-activating, antibody-mediated Platinum Taq DNA Polymerase–complete, rapid activation; provides high sensitivity and reproducibility
• The One-Step SuperScript III Reverse Transcriptase module– a unique formulation to ensure high yield and less PCR inhibition
• Flexible formats with separate or pre-mixed ROX–designed to work with default instrument protocols on a wide range of high-throughput fast instruments (e.g., AB 7500 Fast, AB 7900 HT Fast, AB StepOne, Roche LightCycler™ 480, Corbett Rotor-Gene™)
• Contains heat-labile UDG–significantly reduces carryover contamination risk with excellent activity from room temperature to approximately 40°C. Due to the thermostability of SuperScript III, the cDNA synthesis step is run at 50°C, at which temperature the heat-labile UDG is totally and irreversibly inactivated
EXPRESS kits work with manufacturer’s default protocols on fast instruments and come in a convenient, stable SuperMix format for ultimate convenience. The kits are offered with either pre-mixed ROX or a separate tube of ROX to fit your instrument needs, as well as SYBR Green chemistry options:
EXPRESS One-Step Superscript qRT-PCR Kit, universal (this page)
EXPRESS One-Step Superscript qRT-PCR Kit, with premixed ROX
EXPRESS One-Step SYBR GreenER Kit, with premixed ROX
EXPRESS One-Step SYBR GreenER Kit, universal
Features of the EXPRESS One-Step Superscript qRT-PCR Kit include:
• Fast-activating, antibody-mediated Platinum Taq DNA Polymerase–complete, rapid activation; provides high sensitivity and reproducibility
• The One-Step SuperScript III Reverse Transcriptase module– a unique formulation to ensure high yield and less PCR inhibition
• Flexible formats with separate or pre-mixed ROX–designed to work with default instrument protocols on a wide range of high-throughput fast instruments (e.g., AB 7500 Fast, AB 7900 HT Fast, AB StepOne, Roche LightCycler™ 480, Corbett Rotor-Gene™)
• Contains heat-labile UDG–significantly reduces carryover contamination risk with excellent activity from room temperature to approximately 40°C. Due to the thermostability of SuperScript III, the cDNA synthesis step is run at 50°C, at which temperature the heat-labile UDG is totally and irreversibly inactivated
EXPRESS kits work with manufacturer’s default protocols on fast instruments and come in a convenient, stable SuperMix format for ultimate convenience. The kits are offered with either pre-mixed ROX or a separate tube of ROX to fit your instrument needs, as well as SYBR Green chemistry options:
EXPRESS One-Step Superscript qRT-PCR Kit, universal (this page)
EXPRESS One-Step Superscript qRT-PCR Kit, with premixed ROX
EXPRESS One-Step SYBR GreenER Kit, with premixed ROX
EXPRESS One-Step SYBR GreenER Kit, universal
研究用途にのみご使用ください。診断目的には使用できません。
仕様
使用対象 (装置)7500 Fast System, 7500 System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, BioRad CFX96™, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad MyiQ, Corbett RotorGene, Cepheid SmartCycler, MJ Opticon, MJ Chromo4, BioRad iCycler iQ
フォーマットTube
反応数反応2500回分
ポリメラーゼTaq DNA Polymerase
製品ラインSuperScript™
製品タイプqRT-PCR Kit
数量2,500 reactions
サンプルタイプRNA
対応可能対象2500反応
検出法Primer-probe
PCR法1-step RT-qPCR
反応速度高速または標準
Unit SizeEach
組成および保存条件
The EXPRESS One-Step SuperScript™ qRT-PCR Universal kit contains 3 modules: 2X EXPRESS SuperScript™ Mix (5 × 1 mL), Express qPCR SuperMix Universal (5 × 5 mL), and ROX Reference Dye (5 × 500 μL).
Store at -20°C for long-term storage. Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at 4–8°C.
Store at -20°C for long-term storage. Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at 4–8°C.
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