Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
製品番号(カタログ番号) | ユニットサイズ | 価格(JPY) | 在庫と納期 | |
---|---|---|---|---|
12430054 | 500 mL | 価格 4,200 | ||
12430062 | 10 x 500 mL | 価格 34,800 | ||
12430047 | 1,000 mL | 価格 7,400 | ||
12430104 | 6 x 1,000 mL | 価格 36,300 | ||
12430112 または、製品番号12430-112 | 5 L | お問い合わせください › | *** |
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
With | Without |
• High Glucose | • Sodium Pyruvate |
• L-glutamine | |
• HEPES | |
• Phenol Red |
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.