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Gibco™
B-27™ Supplement (50X), serum free
もっとも引用されている神経細胞培養サプリメントであるB-27サプリメントは、低密度または高密度での成長や、胚、出生後、および成人の海馬やその他のCNS神経細胞の短期または長期の生存率をサポートするために使用される最適化された無血清サプリメントです。B-27サプリメントは50X濃度の液体として提供され、アストロサイトフィーダー層を必要とせずに、神経細胞培養用のNeurobasal培地またはNeurobasal-A培地で使用することを目的としています。B-27サプリメントは、ほとんどの神経細胞アプリケーションに使用できます詳細を見る
製品番号（カタログ番号） 17504001
価格（JPY）お問い合わせください ›
322,000
Each
数量:
100 mL
もっとも引用されている神経細胞培養サプリメントであるB-27サプリメントは、低密度または高密度での成長や、胚、出生後、および成人の海馬やその他のCNS神経細胞の短期または長期の生存率をサポートするために使用される最適化された無血清サプリメントです。B-27サプリメントは50X濃度の液体として提供され、アストロサイトフィーダー層を必要とせずに、神経細胞培養用のNeurobasal培地またはNeurobasal-A培地で使用することを目的としています。
B-27サプリメントは、ほとんどの神経細胞アプリケーションに使用できます。その他のアプリケーション向けに、別の組成のB-27サプリメントおよびNeurobasal培地をご用意しています。
B-27サプリメントを使用して確立されたプロトコルについては、Gibco Neurobiology Protocol Handbookをご覧ください。
Gibco神経生物学製品を使用した出版物については、Gibco CellCiteをご覧ください。
B-27サプリメントは、ほとんどの神経細胞アプリケーションに使用できます。その他のアプリケーション向けに、別の組成のB-27サプリメントおよびNeurobasal培地をご用意しています。
B-27サプリメントを使用して確立されたプロトコルについては、Gibco Neurobiology Protocol Handbookをご覧ください。
Gibco神経生物学製品を使用した出版物については、Gibco CellCiteをご覧ください。
研究用途にのみご使用ください。診断目的には使用できません。
仕様
細胞タイプNeural Cells
濃度10 X
培養タイプMammalian Cell Culture
数量100 mL
血清レベルSerum-Free
品質保持期間12 Months
試験済みEndotoxin, Performance, Sterility, Mycoplasma
分類Serum-free
形状Liquid
製品タイプCell Culture Supplement
無菌性Sterile
Unit SizeEach
組成および保存条件
Store in freezer (-5 to -20°C) and protect from light.
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図
Rat cortical neurons cultured in Neurobasal Medium with B-27 Supplement
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証明書
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ロット番号Certificate TypeDateCatalog Number(s)
2954071Certificate of Analysis2025年6月22日17504044, 17504001
2954081Certificate of Analysis2025年6月22日17504044, 17504001
3135631Certificate of Analysis2025年6月21日17504044, 17504001
2954065Certificate of Analysis2025年5月03日17504044, 17504001
3011455Certificate of Analysis2025年4月23日17504044, 17504001
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Safety Data Sheets
SDSScientific Resources
Product Information
よくあるご質問（FAQ）
Yes, the protein content in this product is high enough so filtering through a low protein binding filter as a 50X or 1X in solution should not be a problem.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .
We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.
For more information on Neurobasal media, search "Neurobasal" from our website home page.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We recommend using Neurobasal Plus Medium with B-27 Plus Supplement for long-term culture of mixed hippocampal cells. For short-term culture of a pure population of hippocampal neurons, we would recommend using Neurobasal-A Medium with B-27 Supplement.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Yes, we do offer B-27 Supplement, minus insulin (Cat. Nos. A1895601 and A1895602). All our other B-27 supplements do contain insulin.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
引用および参考文献 (7)
Search citations by name, author, journal title or abstract text
引用および参考文献
Abstract
A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.
Journal:Experimental cell research
PubMed ID:38499143
In vitro generation of human pluripotent stem cell derived lung organoids.
Journal:
PubMed ID:25803487
'Recent breakthroughs in 3-dimensional (3D) organoid cultures for many organ systems have led to new physiologically complex in vitro models to study human development and disease. Here, we report the step-wise differentiation of human pluripotent stem cells (hPSCs) (embryonic and induced) into lung organoids. By manipulating developmental signaling pathways hPSCs
Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro.
Journal:Nature
PubMed ID:21151107
Studies in embryonic development have guided successful efforts to direct the differentiation of human embryonic and induced pluripotent stem cells (PSCs) into specific organ cell types in vitro. For example, human PSCs have been differentiated into monolayer cultures of liver hepatocytes and pancreatic endocrine cells that have therapeutic efficacy in
Axonal Transport Enables Neuron-to-Neuron Propagation of Human Coronavirus OC43.
Journal:J Virol
PubMed ID:29925652
Human coronaviruses (HCoVs) are recognized respiratory pathogens for which accumulating evidence indicates that in vulnerable patients the infection can cause more severe pathologies. HCoVs are not always confined to the upper respiratory tract and can invade the central nervous system (CNS) under still unclear circumstances. HCoV-induced neuropathologies in humans are
Recapitulation of SARS-CoV-2 infection and cholangiocyte damage with human liver ductal organoids.
Journal:Protein Cell
PubMed ID:32303993
7 total citations