Matrix Standards Kit, BigDye™ Terminator v1.1, for 31xx/3500/SeqStudio™/SeqStudio™ Flex
Matrix Standards Kit, BigDye™ Terminator v1.1, for 31xx/3500/SeqStudio™/SeqStudio™ Flex
Applied Biosystems™

Matrix Standards Kit, BigDye™ Terminator v1.1, for 31xx/3500/SeqStudio™/SeqStudio™ Flex

This kit contains two tubes of standard used to generate a spectrum required when analyzing BigDye Terminator v1.1 labeled DNA詳細を見る
製品番号(カタログ番号)数量
43368241 kit
製品番号(カタログ番号) 4336824
価格(JPY)
30,000
Each
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数量:
1 kit
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This kit contains two tubes of standard used to generate a spectrum required when analyzing BigDye Terminator v1.1 labeled DNA fragments on the 31xx, 3500, SeqStudio, and SeqStudio Flex series genetic analyzers. Each tube contains a blend of four different colors of fluorescent dye-labeled DNA fragments suspended in 1X TE buffer.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
使用対象(アプリケーション)配列
使用対象 (装置)31xx Series Genetic Analyzer, SeqStudio™ Genetic Analyzer, 3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers
標識または色素ROX, TAMRA™, Rhodamine 110, Rhodamine 6G
製品ラインBigDye™ Terminator, BigDye™
製品タイプMatrix Standard
数量1 kit
出荷条件Wet Ice
フォーマットキット
Unit SizeEach
組成および保存条件
Store at 2–8°C protected from light. Do not freeze.
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証明書

ロット番号Certificate TypeDateCatalog Number(s)
3240985Certificate of Analysis2025年5月16日4336824
3204679Certificate of Analysis2025年4月04日4336824
3194816Certificate of Analysis2025年3月21日4336824
2815239Certificate of Analysis2025年1月29日4336824
2835766Certificate of Analysis2025年1月29日4336824
5件の結果が表示されました。 上記から特定の証明書を検索します

Safety Data Sheets

よくあるご質問(FAQ)

Some of the other causes of an Unstable Electrophoresis current detected error message are:

1.Leak on the system
2. Polymer that:
-Has expired
-Has been left on the instrument for more than the recommended time
-Is a mixture of expired polymer and non-expired polymer
3. Running Buffer that was:
-On the instrument longer than 2 weeks
-Not filled to the fill line or evaporated below the fill line
4. An arcing event that was not cleaned afterwards using the water wash wizard
5. Not performing regular maintenance on the instrument
6. Hardware issues

Inspect the system for leaks. If you do not see any leaks on the system, perform the wash the pump chamber and channels wizard using the conditioning pouch and place fresh, non-expired polymer and fresh Anode and Cathode buffer containers. If the problem persists, a service call may be required.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.

If the instrument is not in use, it is not necessary to replace the buffer every 14 days. However, the buffer level needs to remain at the fill line to prevent the capillary or array from drying out. Top off the buffer volume with water if necessary. Replace the anode and cathode buffer containers prior to starting a new run.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.

The spectral calibration on the Applied Biosystems 3130 series systems can be performed by either using a Matrix Standard or a Sequencing Standard. The Matrix Standards will give 4 peaks, each of a different color whereas the Sequencing Standard is pre-sequenced and cleaned DNA that is dried down and just needs to be resuspended.

To make up the Matrix Standard:

  1. Vortex the Matrix Standard tube for 1 min at full speed and pulse spin in a microcentrifuge.
  2. Dilute the Matrix Standard in Hi-Di Formamide in a 1.5 mL microcentrifuge tube according to the following dilutions:
      For 4 capillary arrays
    • 36 cm and 80 cm arrays: mix 2.5 µL of Matrix Standard with 47.5 µL of Hi-Di Formamide.
    • 50 cm array: mix 2.5 µL of Matrix Standard with 97.5 µL of Hi-Di Formamide.

      For 16 capillary arrays
    • 36 cm and 80 cm arrays: mix 10 µL of Matrix Standard with 190 µL of Hi-Di Formamide.
    • 50 cm array: mix 5 µL of Matrix Standard with 195 µL of Hi-Di Formamide.
  3. Mix thoroughly and pulse spin in a microcentrifuge.
  4. Denature at 95 degrees C for 2 min and dispense 10 µL of reagent into wells A1–H12 (16 capillaries) or A1–D1 for 4 capillaries on a 96-well plate.
  5. Briefly spin to ensure there are no air bubbles at the bottom of the well.
  6. Set up and run the spectral calibration as per the Applied Biosystems 3130/3130xl Genetic Analyzers Getting Started Guide


More information on making up the Matrix Standards can be found in the BigDye Terminator v.3.1 Matrix Standard Kit for 3130 and 3100 Series Systems Product Insert or the BigDye Terminator v.1.1 Matrix Standard (Dye Set E) for 3100, 3130, and 3500 Series Genetic Analyzers Product Insert

To make up the Sequencing Standard:
  1. Resuspend one tube of the Sequencing Standard with 170 µl of Hi-Di Formamide.
  2. Vortex at full speed for 1 min and pulse spin in a microcentrifuge.
  3. Denature at 95 degrees C for 2 min and dispense 10 µL of reagent into wells A1–H12 (16 capillaries) or A1–D1 for 4 capillaries on a 96-well plate.
  4. Briefly spin to ensure there are no air bubbles at the bottom of the well.
  5. Set up and run the spectral calibration as per the Applied Biosystems 3130/3130xl Genetic Analyzers Getting Started Guide


Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.

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