TaqMan™ Gene Expression Master Mix
TaqMan™ Gene Expression Master Mix
Applied Biosystems™

TaqMan™ Gene Expression Master Mix

Get precise and reliable real-time qPCR quantification over nine logs of linear dynamic range with TaqMan Gene Expression Master Mix.詳細を見る
製品番号(カタログ番号)数量
43700481 x 1 mL
436954210 x 5 mL
43695142 x 5 mL
43695105 x 5 mL
43690161 x 5 mL
43700741 x 50 mL
製品番号(カタログ番号) 4370048
価格(JPY)
18,900
Each
お問い合わせください ›
数量:
1 x 1 mL
Get precise and reliable real-time qPCR quantification over nine logs of linear dynamic range with TaqMan Gene Expression Master Mix. TaqMan Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target. Extended benchtop stability provides consistent results for automated liquid-handling systems, making it the choice for high-throughput sample processing, overnight or over the weekend. TaqMan Gene Expression Master Mix has been validated with TaqMan gene expression assays and Applied Biosystems real-time systems, providing precise quantification for a variety of gene expression–based real-time qPCR applications, including:
• Rare transcript detection
• Gene expression analysis
• Pathogen detection and viral load quantification
• Co-amplification of two targets
• Specific detection of gene family members

TaqMan Gene Expression Master Mix contains AmpliTaq Gold DNA Polymerase, UP (Ultra Pure), for hot start activation and improved detection of bacterial targets, a blend of dNTPs with dTTP/dUTP and uracil-DNA glycosylase (UDG) to minimize carry-over PCR contamination, and a passive internal reference based on proprietary ROX dye for superb precision on Applied Biosystems real-time PCR instruments.

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Gene Expression Master Mix and added additional capabilities for your gene expression analysis.

研究用途にのみご使用ください。診断目的には使用できません。
仕様
使用対象 (装置)7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR SystemStepOne™, Standard Mode, StepOnePlus™, Standard Mode
反応数反応40回分
受動的参照色素ROX(プレミックス)
ポリメラーゼAmpliTaq Gold DNA Polymerase
製品ラインTaqMan™
純度または品質グレード高(超高純度)
数量1 x 1 mL
サンプルタイプDNA (Genomic), cDNA
容量1 x 1 mL
検出法Primer-probe
使用対象(アプリケーション)遺伝子発現解析
形状液体
PCR法qPCR
反応速度スタンダード
Unit SizeEach
組成および保存条件
1 tube (1 mL) 2X TaqMan™ Gene Expression Master Mix sufficient for 40 reactions. Store at 2°C to 8°C.


TaqMan™ Gene Expression Master Mix contains AmpliTaq Gold™ DNA Polymerase (Ultra Pure), Uracil-DNA glycosylase, dNTPs (with dUTP), ROX™ Passive Reference, and optimized buffer components.
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よくあるご質問(FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.

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