Applied Biosystems™

SYBR™ Select Master Mix

製品番号(カタログ番号): 4472918
Applied Biosystems™

SYBR™ Select Master Mix

製品番号(カタログ番号): 4472918
製品番号(カタログ番号)
4472918
ユニットサイズ
2 x 5 mL
価格(JPY)
在庫と納期
製品番号(カタログ番号)
ユニットサイズ
1 x 1 mL
価格(JPY)
在庫と納期
製品番号(カタログ番号)
ユニットサイズ
1 x 5 mL
価格(JPY)
在庫と納期
製品番号(カタログ番号)
ユニットサイズ
5 x 5 mL
価格(JPY)
在庫と納期
製品番号(カタログ番号)
ユニットサイズ
10 x 5 mL
価格(JPY)
在庫と納期
製品番号(カタログ番号)
ユニットサイズ
1 x 50 mL
価格(JPY)
在庫と納期
製品番号(カタログ番号)ユニットサイズ価格(JPY)在庫と納期
44729182 x 5 mL
44729031 x 1 mL
44729081 x 5 mL
44729195 x 5 mL
447292010 x 5 mL
44729131 x 50 mL
製品概要
ビデオ
推奨事項
推奨事項
参考資料
FAQ
引用および参考文献
その他の情報
推奨事項
SYBR™ Select Master Mix is a superior and cost-effective solution for your real-time PCR applications.

Features of the SYBR™ Select Master Mix include:

• Specific—minimize primer-dimer and non-specific amplification
• Reproducible and sensitive—consistent amplification across a wide dynamic range
• Bright—contains SYBR™ GreenER™ dye for maximum brightness
• Carry-over contamination control—contains heat-labile UDG
• Can be used in either standard or fast cycling mode

Formulated for Maximum Specificity
SYBR™ Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq™ DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.

Obtain Reproducible Results Across a Wide Dynamic Range
SYBR Select Master Mix is specially formulated to provide robust results at 100 ng to 0.1 pg cDNA per reaction (Figure 1) and can reliably detect a single copy of genomic DNA (Figure 2).

Contains SYBR™ GreenER™ Dye and Heat-labile UDG
• SYBR™ GreenER™ dye is less inhibitory to PCR than SYBR™ Green I dye resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

Instrument Compatibility
SYBR™ Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments except the 7900HT real-time PCR system. It is also compatible with the Bio-Rad IQ™5, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems.

View performance data.

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR Select Master Mix and added additional capabilities for your gene expression analysis.
For Research Use Only. Not for use in diagnostic procedures.

仕様

検出法
SYBR
形状
Liquid
フォーマット
Vial
GC-Rich PCR Performance
High
PCR法
qPCR
ポリメラーゼ
AmpliTaq DNA Polymerase
反応速度
Fast or Standard
使用対象 (装置)
7500 Fast System, 7500 System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Fast Mode, StepOne™, Standard Mode, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System, AB StepOnePlus™, Fast Mode, AB StepOne™, Standard Mode, AB 7500, Fast Mode, AB 7500, Standard Mode, AB StepOne™, Fast Mode, AB StepOnePlus™, Standard Mode
標識または色素
SYBR GreenER
受動的参照色素
ROX (Pre-mixed)
製品ライン
SYBR™
製品タイプ
Real Time PCR SYBR Master Mix
純度または品質グレード
UP (Ultra Pure)
数量
2 x 5 mL
対応可能対象
100 Reactions of 20 μL
使用対象(アプリケーション)
Gene Expression
濃度
2X
ホットスタート
Built-In Hot Start
反応数
1000 Reactions
サンプルタイプ
DNA (Genomic), cDNA
容量
2 x 5 mL

組成および保存条件

The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 μL reactions (10 μL Master Mix per reaction). Store at 2–8°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems.

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