Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL
Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL
Thermo Scientific™

Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL

Thermo Scientific Zebaスピン脱塩プレート(7K MWCO)には、2 µL~4 mLのタンパク質サンプルの遠心分離で優れた脱塩性能を発揮するサイズ排除クロマトグラフィーレジンが充填されています。Zebaスピン脱塩プレートの特長:•高い回収率詳細を見る
製品番号(カタログ番号)数量フォーマット容量(メートル法)
8988225カラム微量遠心機、スピンカラム0.5 mL
8987725カラム微量遠心機、スピンカラム75 μL
8987850カラム微量遠心機、スピンカラム75 μL
A400024695カラムSpin Column20 mL
A4000247510 ColumnsSpin Column20 mL
8989025カラム15 mLコニカル、スピンカラム2 mL
898915カラム15 mLコニカル、スピンカラム5 mL
8989225カラム15 mLコニカル、スピンカラム5 mL
898935カラム50 mLコニカル、スピンカラム10 mL
8989425カラム50 mLコニカル、スピンカラム10 mL
898895カラム15 mLコニカル、スピンカラム2 mL
8988350カラム微量遠心機、スピンカラム0.5 mL
899345カラムクロマトグラフィーカートリッジ1 mL
899355カラムクロマトグラフィーカートリッジ5 mL
89807プレート2枚96-ウェルフィルタープレート96-well
89808プレート4枚96-ウェルフィルタープレート96-well
製品番号(カタログ番号) 89882
価格(JPY)
33,000
Each
お問い合わせください ›
数量:
25カラム
フォーマット:
微量遠心機、スピンカラム
容量(メートル法):
0.5 mL
一括またはカスタム形式をリクエストする
Ask our AI about this Product
Thermo Scientific Zebaスピン脱塩プレート(7K MWCO)には、2 µL~4 mLのタンパク質サンプルの遠心分離で優れた脱塩性能を発揮するサイズ排除クロマトグラフィーレジンが充填されています。

Zebaスピン脱塩プレートの特長:

高い回収率 — 低結合性レジンでタンパク質の回収率を最大化します
高速 — 分画のスクリーニングが不要。重力フローによるタンパク質出現を待つこともありません
経済的 — 他の市販のカートリッジよりも低コストで優れた性能を発揮します

Zeba脱塩プレートは、高性能のZeba脱塩レジンを含有するポリプロピレン製装置で、迅速で優れたタンパク質脱塩を行うとともに、7,000ダルトンを超えるタンパク質を高率で回収します。Zeba脱塩装置は、2 µL~4 mLのサンプルの脱塩とバッファー交換を簡単に実行できます。この96ウェルスピンプレートフォーマットにより、よりハイスループットな処理が可能になります。

Zeba 96ウェルスピン脱塩プレートは、レジンの分注や膨潤が不要で、スピンカラムと同等の高タンパク質回収率を実現し、塩や他の低分子を95%以上除去します。1枚のプレートで96個の少量サンプル(20~100 µL)を5分で処理できます。

詳細な製品データ
Zebaスピン脱塩カラムを用いたベンチマーク実験
脱塩精製、アフィニティー精製のためのクロマトグラフィーカートリッジ

関連製品
Zeba™ マイクロスピン脱塩カラム、7K MWCO、75 µL
Zeba™スピン脱塩カラム、7K MWCO、0.5 mL
Zeba™スピン脱塩カラム、7K MWCO、2 mL
Zeba™スピン脱塩カラム、7 K MWCO、5 mL
Zeba™スピン脱塩カラム、7 K MWCO、10 mL
For Research Use Only. Not for use in diagnostic procedures.
仕様
製品タイプスピン脱塩カラム
精製標的緩衝液交換、タンパク質
数量25カラム
容量(メートル法)0.5 mL
カラムタイプサイズ排除、特許取得済みレジン
フォーマット微量遠心機、スピンカラム
MWCO7.0 kDa
製品ラインZeba™
サンプル量(メートル法)30 to 130 μL
Unit SizeEach
組成および保存条件
受け取り後4℃で保存。
Have questions about this product? Ask our AI assisted search.
Generating response
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

ビデオ

Zeba Protein Desalting Spin Columns and 96-Well Spin Plates

Video Player is loading.
Current Time 0:00
Duration 0:00
Loaded: 0%
Stream Type LIVE
Remaining Time 0:00
 
1x
    • Chapters
    • descriptions off, selected
    • captions off, selected

      Kunden, die diesen Artikel ansahen, interessierten sich auch für



      ドキュメントおよびダウンロード

      証明書

      ロット番号Certificate TypeDateCatalog Number(s)
      3213623Certificate of Analysis2025年7月03日89882
      3217131Certificate of Analysis2025年7月03日89934
      3229509Certificate of Analysis2025年7月03日89935
      3267228Certificate of Analysis2025年7月01日89892
      3267228ACertificate of Analysis2025年7月01日89892
      5件の結果が表示されました。 上記から特定の証明書を検索します

      Safety Data Sheets

      よくあるご質問(FAQ)

      You may remove excess solvent and smaller moieties by centrifugation through a microconcentrator. This will concentrate your protein sample.

      (1) Choose microconcentrator tube (available from a variety of commercial suppliers) with a protein cutoff smaller than the molecular weight of the protein in the sample. Check our Pierce Protein Concentrators PES.

      (2) Add 1 µL of 20% w/v SDS to a dry microconcentrator tube (if sample does not already contain SDS).

      (3) Slowly add sample (a few microliters at a time) to membrane until membrane is completely wet. Centrifuge to near (but not complete) dryness.

      (4) If intention is to desalt sample or buffer exchange: Add ~50 µL water to microconcentrator and spin until nearly dry. Repeat buffer exchange. Sample will remain on membrane. Check our Zeba desalting proteins.

      (5) Using a new collection tube, invert membrane and spin at low speed (1000 x g) to elute protein from membrane. Add 2X SDS-Sample Buffer containing 10 mM DTT to membrane: vortex, invert and spin. Final volume should be ~20 µL.

      Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

      Yes, Zeba Desalting columns, plates, and cartridges can be autoclaved at 115 degrees C for 30 min.

      Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

      Zeba Desalting columns, plates, and cartridges are compatible with a range of reagents and conditions. They have been confirmed to be compatible with the following:
      - pH range of 3-10
      - Mild oxidants/reductants
      - Chaotropes (stable in 8 M urea and 6 M guanidine HCl)
      - Salts
      - Alcohols up to 20%
      - Organic solvents such as dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) (we recommend using a step-gradient of increasing concentration of the organic solvent during column equilibration)

      Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

      We recommend storing Zeba Spin Desalting Columns, Plates, and Cartridges at 4 degrees C.

      Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

      We recommend using Zeba Spin Desalting Columns, Plates, and Cartridges (Cat. No. 89882, 89883) to remove sodium azide from the Alix Monoclonal Antibody (3A9) storage solution.

      Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

      引用および参考文献 (15)

      引用および参考文献
      Abstract
      Cardioprotective O-GlcNAc signaling is elevated in murine female hearts via enhanced O-GlcNAc transferase activity.
      Authors:Narayanan B,Sinha P,Henry R,Reeves RA,Paolocci N,Kohr MJ,Zachara NE
      Journal:The Journal of biological chemistry
      PubMed ID:37949223
      The post-translational modification of intracellular proteins by O-linked β-GlcNAc (O-GlcNAc) has emerged as a critical regulator of cardiac function. Enhanced O-GlcNAcylation activates cytoprotective pathways in cardiac models of ischemia-reperfusion (I/R) injury; however, the mechanisms underpinning O-GlcNAc cycling in response to I/R injury have not been comprehensively assessed. The cycling of ... More
      Ethinylestradiol in combined hormonal contraceptive has a broader effect on serum proteome compared with estradiol valerate: a randomized controlled trial.
      Authors:Kangasniemi MH,Arffman RK,Joenväärä S,Haverinen A,Luiro K,Tohmola T,Renkonen R,Heikinheimo O,Tapanainen JS,Piltonen TT
      Journal:Human reproduction (Oxford, England)
      PubMed ID:36416543
      STUDY QUESTION: Does an estradiol-based combined oral contraceptive (COC) have a milder effect on the serum proteome than an ethinylestradiol (EE)-based COC or dienogest (DNG) only? SUMMARY ANSWER: The changes in serum proteome were multifold after the use of a synthetic EE-based COC compared to natural estrogen COC or progestin-only ... More
      Antibody-dependent enhancement of toxicity of myotoxin II from Bothrops asper.
      Authors:Sørensen CV,Fernández J,Adams AC,Wildenauer HHK,Schoffelen S,Ledsgaard L,Pucca MB,Fiebig M,Cerni FA,Tulika T,Voldborg BG,Karatt-Vellatt A,Morth JP,Ljungars A,Grav LM,Lomonte B,Laustsen AH
      Journal:Nature communications
      PubMed ID:38228619
      Improved therapies are needed against snakebite envenoming, which kills and permanently disables thousands of people each year. Recently developed neutralizing monoclonal antibodies against several snake toxins have shown promise in preclinical rodent models. Here, we use phage display technology to discover a human monoclonal antibody and show that this antibody causes antibody-dependent ... More
      Ubiquitin-driven protein condensation stabilizes clathrin-mediated endocytosis.
      Authors:Yuan F,Gollapudi S,Day KJ,Ashby G,Sangani A,Malady BT,Wang L,Lafer EM,Huibregtse JM,Stachowiak JC
      Journal:PNAS nexus
      PubMed ID:39253396
      Clathrin-mediated endocytosis is an essential cellular pathway that enables signaling and recycling of transmembrane proteins and lipids. During endocytosis, dozens of cytosolic proteins come together at the plasma membrane, assembling into a highly interconnected network that drives endocytic vesicle biogenesis. Recently, multiple groups have reported that early endocytic proteins form ... More
      Microscale Thermophoresis (MST) as a Tool to Study Binding Interactions of Oxygen-Sensitive Biohybrids.
      Authors:Jagilinki BP,Willis MA,Mus F,Sharma R,Pellows LM,Mulder DW,Yang ZY,Seefeldt LC,King PW,Dukovic G,Peters JW
      Journal:Bio-protocol
      PubMed ID:39131194
      Microscale thermophoresis (MST) is a technique used to measure the strength of molecular interactions. MST is a thermophoretic-based technique that monitors the change in fluorescence associated with the movement of fluorescent-labeled molecules in response to a temperature gradient triggered by an IR LASER. MST has advantages over other approaches for ... More
      15 total citations

      Andere Produkte



      こちらの製品には複数の製品番号が設定されています。

      1x1 image pixel for data collection