resDNASEQ™ Quantitative HEK293 DNA Kits
resDNASEQ™ Quantitative HEK293 DNA Kits
Applied Biosystems™

resDNASEQ™ Quantitative HEK293 DNA Kits

The resDNASEQ Quantitative HEK293 DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host-cell DNA from HEK293 cell lines.
製品番号(カタログ番号)内容
A46014Assay Kit only
A46565Assay Kit and Sample Preparation
製品番号(カタログ番号) A46014
価格(JPY)
447,900
Each
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内容:
Assay Kit only
The resDNASEQ Quantitative HEK293 DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host-cell DNA from HEK293 cell lines used in the development of gene therapy, cell-based vaccines, and similar biotherapeutics. Reliable and rapid, the resDNASEQ system enables sensitive and specific quantitation of HEK293 cell DNA. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types, from in-process samples with different sample matrices to purified final product.
Features of the resDNASEQ Quantitative HEK293 DNA Kit include:
• Highly sensitive quantitation using proven TaqMan real-time qPCR technology
• Consistent performance across the expected range of DNA fragment sizes
• Includes master mix, TaqMan primer/probe mix, and HEK293 genomic DNA standard

This kit is available with or without sample preparation reagents in the form of a PrepSEQ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts.

Integrated workflow solution
The resDNASEQ Quantitative HEK293 DNA Kit is part of an integrated workflow for impurity and contaminant testing during biopharmaceutical manufacturing. The kit is compatible with the PrepSEQ Residual DNA Sample Preparation Kit, which offers both a manual and automated sample preparation workflow. The resDNASEQ kit has been validated on the Applied Biosystems 7500 Fast Real-Time PCR System and the Applied Biosystems QuantStudio 5 Real-Time PCR System. Data analysis is streamlined using AccuSEQ Real-Time PCR Detection Software that provides accurate quantitation and security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance.

Easy to use
With the resDNASEQ Quantitative HEK293 DNA Kit, you perform qPCR analysis to compare the amount of HEK293 DNA in your test samples to a standard curve generated with known amounts of purified HEK293 DNA standard. The resDNASEQ kit includes almost everything you need to carry out these steps, from master mix to TaqMan primer/probe mix to HEK293 genomic DNA standard, as well as step-by-step instructions.

Consistent performance
For accurate quantitation of residual HEK293 DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ system you get consistent assay performance across a range of DNA fragment sizes.

*Due to the high degree of similarity between genomes, DNA from closely-related primate species, such as monkey (Vero), chimpanzee, and orangutan, are expected be detected by the resDNASEQ HEK293 Quantitation Kit.

For Research Use Only. Not for use in diagnostic procedures.
仕様
フォーマットキット
宿主細胞HEK293
内容Assay Kit only
反応数反応100回分
製品ラインresDNASEQ™
数量100 reactions
出荷条件ドライアイス
検出法蛍光
標識または色素FAM
PCR法qPCR
Unit SizeEach
組成および保存条件
resDNASEQ HEK293 DNA Control
• HEK293 DNA Control、30 ng/μL、40 μL、–15~–25℃で保存
• DNA Dilution Buffer(DDB)、7 mL、初回使用前は–15~–25℃、初回使用後は2~8℃で保存

resDNASEQ HEK293 DNA Real-Time PCR Reagents
•2☓Environmental Master Mix、2☓0.75 mL、初回使用前は–15~–25℃C、初回使用後は2~8℃で保存、要遮光
• 10☓HEK293 DNA Assay Mix、300 μL、–15~–25℃で保存、要遮光
• Negative Control(水)、1.0 mL、初回使用前は–15~–25℃、初回使用後は2~8℃で保存

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