The Oncomine IGH FR1-J Assay is a targeted next-generation sequencing (NGS) assay for detection of somatic hypermutation (SHM) in the variable region of the immunoglobulin heavy chain of B-cell receptors. SHM increases the affinity of the B-cell receptor for antigen, post-VDJ recombination, and is frequently a region of interest in CLL research. Primers have been specifically designed to target framework 1 and joining gene regions of the immunoglobulin heavy chain, resulting in an amplicon ∼300 bp in length. When combined with Ion Reporter Software, you can easily analyze your data and create reports within a short period of time.
Key features of the Oncomine IGH FR1-J Assay: • Sequencing of FR1-J region of IGH chain • Detects SHM in CLL samples with high degree of sensitivity • Optimized Oncomine informatics for easy, all-inclusive analysis and publication-ready data plots
The kit provides reagents for library construction, including dNTPs. Ion AmpliSeq libraries can be generated from DNA extracted from fresh/frozen tissue, bone marrow, whole blood, peripheral blood leukocytes, PBMCs, and sorted B cells with as little as 50 ng of nucleic acid per pool. The assay is optimized for sequencing on the Ion GeneStudio S5 System with the Ion 530 Chip to enable up to eight samples per run.
For Research Use Only. Not for use in diagnostic procedures.
仕様
コントロールテンプレート
dNTP mix
制御タイプ
Ion AmpliSeq Library Plus Kit
サンプルタイプ
DNA
Sequencing Type
Genome and DNA Sequencing
使用対象 (装置)
GeneStudio
使用対象(アプリケーション)
Sequencing
ライブラリ
targeted sequencing
反応数
24
出荷条件
Dry Ice
組成および保存条件
• Oncomine IGH FR1-J Panel • dNTP mix • Ion AmpliSeq Library Plus Kit All components stored at -10 to -30°