Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
製品番号(カタログ番号) | 数量 |
---|---|
B40931 | 150 Slides |
To limit background, we recommend performing a pre-blocking step with 3% H2O2 for 60 mins to inactivate endogenous peroxidases. To limit the localization of labeling, we recommend optimizing the final concentration of the primary and secondary antibodies and the dye-tyramide. You may also limit the incubation time of the dye-tyramide on the sample.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Yes. This involves the sequential application of the antibodies and the tyramides with a HRP-quenching step between antibodies using H2O2.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
The SuperBoost tyramide kits utilize poly-HRP labeled antibodies. This provides a greater number of horseradish peroxidase (HRP) molecules per antibody. The original kits used antibodies and streptavidin that were directly conjugated with HRP and thus, limited the number per antibody or streptavidin.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
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