Thermo Scientific Phire植物ダイレクトPCRキットは、植物サンプルから直接DNAを増幅するために設計されています。PCRの前にDNAの精製を行なう必要はありません。このキットは、DNA結合ドメインを持つ特別に設計された酵素であるPhireホットスタートII DNAポリメラーゼをベースにしています。The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant, fungi and bacteria.

The kit includes a complete set of optimized PCR reagents as well as detailed protocols for different sample types, making it an ideal choice for amplification of plant DNA.In addition, the kit includes control primers for positive control reactions.The Control Primers amplify a highly conserved region of chloroplast DNA and are universal for many plant species.

Highlights

• No need for time-consuming and expensive DNA purification steps
• Very little sample material required
• Two simple protocols for various applications
• PhireホットスタートII DNAポリメラーゼ provides high yields of specific product with short extension time (20 s/kb).

Applications

• Genotyping
• Transgene detection

The optimal annealing temperature for Phire DNA Polymerase may differ significantly from that of Taq -based polymerases.最適な結果を得るには、まず当社のTm計算機を使用して、Tmを正確に計算します。