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Invitrogen™
293FT Cell Line
293FT 細胞株 は、ViraPower™ Lentiviral Expression System を使用した高力価レンチウイルスの生成に最適です。293FT 細胞株は詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| R70007 | 1 x 10^7細胞 |
製品番号（カタログ番号） R70007
価格（JPY）お問い合わせください ›
95,500
Each
数量:
1 x 10^7細胞
293FT 細胞株 は、ViraPower™ Lentiviral Expression System を使用した高力価レンチウイルスの生成に最適です。293FT 細胞株は、SV40 ラージ T 抗原で形質転換されたヒト胚性腎細胞から得られた、急速に増殖し、高度にトランスフェクト可能なクローンアイソレートです。ViraPower™ 発現ベクターと ViraPower™ Lentiviral Packaging Mix を 293FT 細胞に同時トランスフェクトすると、パッケージングに必要な高レベルのウイルス RNA と gag⁄pol および rev タンパク質が生成されます。
利点
• 急速な増殖
•高いトランスフェクト性。
•高いウイルス力価を生成します。
•非常に高レベルなタンパク質の生成が可能です。
主な特徴
•SV40 ラージ T 抗原の存在により、SV40 由来のベクターから非常に高レベルのタンパク質を発現させることができます。実際、他の 293 細胞や従来から使用されている T抗原で形質転換された COS 細胞よりも高レベルです。
キットには、90％完全培地 1 mLと 10％ DMSO 内の凍結保存用ストックとして提供される 293FT 細胞株が含まれます。
•
利点
• 急速な増殖
•高いトランスフェクト性。
•高いウイルス力価を生成します。
•非常に高レベルなタンパク質の生成が可能です。
主な特徴
•SV40 ラージ T 抗原の存在により、SV40 由来のベクターから非常に高レベルのタンパク質を発現させることができます。実際、他の 293 細胞や従来から使用されている T抗原で形質転換された COS 細胞よりも高レベルです。
キットには、90％完全培地 1 mLと 10％ DMSO 内の凍結保存用ストックとして提供される 293FT 細胞株が含まれます。
•
研究用途にのみご使用ください。あらゆる治療もしくは診断用には使用できません。
仕様
推奨培地SFM (Serum Free Medium)
セル数1 x 107
製品ラインViraPower™
数量1 x 10^7細胞
細胞株293FT
種ヒト
Unit SizeEach
組成および保存条件
• 293FT 細胞株（液体窒素で保存）
Have questions about this product? Ask our AI assisted search.
Do you recommend a specific FBS for culture of the 293FT or 293A cells used in the ViraPower kits? What plastic plates do you recommend?
What are syncytia?
What are the advantages of the lentiviral system?
What titers are typical with lentivirus?
How do I concentrate the lentiviral stock?
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ドキュメントおよびダウンロード
Safety Data Sheets
SDSScientific Resources
Product Information
LULL (Limited Use of Label License)
'
Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased amount of the product and progeny, to perform internal research and development for the sole benefit of the purchaser. The purchase of this product does not grant the purchaser any additional rights, including, without limitation: (a) the right to use the product or its components in commercial applications of any kind, including bioproduction (e.g. use of the product to manufacture therapeutic agents or diagnostic test components, such as proteins, antibodies, viral particles or virus-like particles), quality control and commercial services such as reporting the results of purchaser's activities for a fee or other form of consideration; (b) the right to use the product or its components as a therapeutic agent or diagnostic test component; (c) the right to use the product or its components to produce material for use in human clinical trials; or (d) the right to transfer or resell the product or its components in any form, progeny or derivative. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.
'
よくあるご質問（FAQ）
If 293FT cells detach shortly after transfection (4 hours to overnight):
- This may be a sign of Lipofectamine 2000 toxicity. Cells may have been plated too sparsely prior to transfection.
- The cells may not have been handled gently enough (these cells have a tendency to lift off easily).
- The cells may have been kept at room temperature for too long.
If cells detach 48 to 72 hours post-transfection:
- If the cells lift off in large sheets, this may be a sign of lentivirus production.
Syncytia are large multi-nucleated cells that result from VSV-G-induced fusion with neighboring 293FT producer cells. Syncytia production is indicative of high transfection efficiency and lentivirus production. Keep in mind, though, that the absence of syncytia does not mean that virus will not be produced.
Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.
The 293FT cell line stably expresses the SV40 large T antigen from the pCMVSPORT6Tag.neo plasmid that contains the neomycin resistance marker. In order to maintain the plasmid/phenotype, the cells have to be routinely cultured in medium containing Geneticin (G418) antibiotic at a concentration of 500 µg/mL.
We use Mycoplasma-tested Gibco FBS (Cat. No. 16000-044). We have observed that when 293FT cells are cultured in the presence of this FBS following the instructions in the manual, virus production is better than that obtained with many other serum sources. We use the following plasticware for 293FT cells:
T175-Fisher Cat. No. 10-126-13; this is a Falcon flask with 0.2 µm vented plug seal cap.
T75-Fisher Cat. No. 07-200-68; this is a Costar flask with 0.2 µm vented seal cap.
100 mm plate-Fisher Cat. No. 08-772E; this is a Falcon tissue culture-treated polystyrene plate.
We get excellent adherence on these plates under routine cell culture/maintenance conditions.
During lentivirus production, 293FT cells undergo the following morphological changes:
- They become multi-nucleated (syncytia development)
- They start to look like balloons or as if they are about to explode
- They often, but not always, lift off from the surface
- Untransfected 293FT cells leave empty spaces and “pile” up at other spots in the flask
Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.
引用および参考文献 (3)
Search citations by name, author, journal title or abstract text
引用および参考文献
Abstract
The interaction between HIV-1 Gag and human lysyl-tRNA synthetase during viral assembly.
Journal:J Biol Chem
PubMed ID:12756246
'Human lysyl-tRNA synthetase (LysRS) is a tRNA-binding protein that is selectively packaged into HIV-1 along with its cognate tRNALys isoacceptors. Evidence exists that Gag alone is sufficient for the incorporation of LysRS into virions. Herein, using both in vitro and in vivo methods, we begin to map regions in Gag
RIP140-targeted repression of gene expression in adipocytes.
Journal:Mol Cell Biol
PubMed ID:16227589
'Ligand-dependent repression of nuclear receptor activity forms a novel mechanism for regulating gene expression. To investigate the intrinsic role of the corepressor RIP140, we have monitored gene expression profiles in cells that express or lack the RIP140 gene and that can be induced to undergo adipogenesis in vitro. In contrast
A novel method for long term bone marrow culture and genetic modification of murine neutrophils via retroviral transduction.
Journal:J Immunol Methods
PubMed ID:19010330
'Neutrophils are a critical component of the innate immune response to invading microbial pathogens. However, an excessive and/or prolonged neutrophil response can result in tissue injury that is thought to underlie the pathogenesis of various inflammatory diseases. The development of novel therapeutic strategies for inflammatory diseases depends on an improved
3 total citations