M13KO7 Helper Phage is suitable for use with M13/phagemid hosts for the production of ssDNA from vectors containing an f1or f1-like origin of replication. Prepared by a modification of the procedure of Vieira and Messing (1), this high-titer helper phage eliminates the need to make and maintain a separate stock of helper phage. It contains the Km^r gene and a p15A origin of replication that allows it to replicate like a plasmid inside the cells. Detailed protocols for the production of ss DNA are provided on the product profile. M13KO7 Helper Phage offers:

• Convenient preparation of ss DNA from M13 or phagemid vectors
• >1 x 10¹¹ pfu/ml