RPMI 1640 Medium, powder
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Gibco™

RPMI 1640 Medium, powder

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as aRead more
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Catalog NumberQuantity
51800035
also known as 51800-035
10 L
Catalog number 51800035
also known as 51800-035
Price (KRW)
-
Quantity:
10 L
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Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. Life Technologies offers a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This RPMI 1640 is modified as follows:
WithWithout
• L-glutamine• HEPES
• Phenol Red• Sodium Bicarbonate


The complete formulation is available.

Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco™ L-15 cell culture medium require sodium bicarbonate supplementation pH adjustment, and filtration at the time of preparation (see protocol for details).

Specifications
Cell TypeMammalian Cells
ClassificationAnimal Origin-free
FormPowder
Product TypeRPMI 1640
Serum LevelStandard Serum Supplementation
Shelf Life36 Months
With AdditivesGlutamine, Phenol Red
Without AdditivesNo Sodium Pyruvate, No Sodium Bicarbonate, No HEPES
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Quantity10 L
Shipping ConditionRoom Temperature
Osmolality230 - 280 mOsm/kg
pH7.4 to 7.6
Culture EnvironmentCO2
Culture TypeMammalian Cell Culture
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C.
Shipping conditions: Ambient.
Shelf life: 36 months from date of manufacture.
Media Formulations
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Frequently asked questions (FAQs)

How light sensitive is RPMI 1640 media? Should I also be protecting it from LED light?

While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the density (g/L) for RPMI 1640 Medium?

We have specific gravity information for RPMI 1640 Medium: 1.006 kg/L. In this case, the specific gravity is the same as density as the solvent is water.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of my powdered media once I reconstitute it?

Expiration date of most Gibco reconstituted dry format media (AGT or DPM) has not been established; end users should assess performance and stability of this reconstituted media in a system that is relevant to their process. A dry format product (either DPM or AGT) will age upon storage and while we may not be able to detect which component(s) degrade since we can't measure every component, that doesn't mean that the reconstituted liquid will have the same stability as a fresh liquid made by direct weigh methods.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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Lot #Certificate TypeDateCatalog Number(s)
3220753Certificate of AnalysisMay 22, 202551800019, 51800043, 51800035
3220757Certificate of AnalysisMay 21, 202551800019, 51800043, 51800035
3075874Certificate of AnalysisApr 19, 202551800019, 51800043, 51800035
3129325XCertificate of AnalysisApr 11, 202551800019, 51800043, 51800035
3160922Certificate of AnalysisMar 06, 202551800019, 51800043, 51800035
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