Thermo Scientific Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA (see Figure 1 in Supporting Data). The enzyme shows no activity on RNA.
Highlights
• Active in Thermo Scientific buffers for restriction enzymes and thermophilic polymerases
Applications
• Control of carry-over contamination in PCR • Glycosylase mediated single nucleotide polymorphism detection (GMPD) • Site-directed mutagenesis • As a probe for protein-DNA interaction studies • SNP genotyping • Cloning of PCR products • Generation of single strand overhangs of PCR products and cDNA
Note
The abasic sites formed in DNA by Uracil-DNA Glycosylase may be subsequently cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites. UDG (UNG) is active in the presence or absence of divalent cations.
Use of this enzyme in certain applications may be covered by patents and may require a license.
For Research Use Only. Not for use in diagnostic procedures.
規格
Concentration
1 U/μL
Enzyme
Uracil-DNA Glycosylase
Compatible Buffer
10X Reaction Buffer
Quantity
5 x 200 U
Product Type
Uracil DNA Glycosylase
Unit Size
5 x 200 units
內容物與存放
Uracil-DNA glycolase (1 U/μL) and 10X Reaction Buffer. Store at -20°C.