Thermo Scientific Lambda Exonuclease is a highly processive 5'→3' exodeoxyribonuclease. It selectively digests the 5'-phosphorylated strand of double-stranded DNA. The enzyme exhibits low activity on single-stranded DNA and non-phosphorylated DNA, and has no activity at nicks and limited activity at gaps in DNA.

Highlights

• Active in Thermo Scientific PCR buffers

Applications
• Generating single-stranded PCR products for use in: • DNA sequencing
• Analysis of DNA single-strand conformation polymorphism (SSCP)
• Rolling circle amplification

• Producing single-stranded DNA from double-stranded DNA fragments
• Cloning of PCR products

Note

Use of this enzyme in certain applications may be covered by patents and may require a license.