MitoSOX™ Mitochondrial Superoxide Indicators, for live-cell imaging
MitoSOX™ Mitochondrial Superoxide Indicators, for live-cell imaging
Invitrogen™

MitoSOX™ Mitochondrial Superoxide Indicators, for live-cell imaging

MitoSOX superoxide indicators are novel fluorogenic dyes specifically targeted to mitochondria in live cells. Oxidation of the MitoSOX reagent by mitochondrial superoxide produces bright green or red fluorescence.
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產品號碼QuantityExcitation/EmissionIndicatorColor
M3600810 vials x 50 μg∼396/610 nmMitoSox RedRed
M360051 vial x 9 μg∼488/510 nmMitoSox GreenGreen
M360065 vials x 9 μg∼488/510 nmMitoSox GreenGreen
M360071 vial x 50 μg∼396/610 nmMitoSox RedRed
M360092 Vials (1 Green, 1 Red)488/510 nm, 396/610 nmMitoSox Green, MitoSox RedGreen, Red
產品號碼 M36008
價格 (TWD)
15,840.00
Online offer
Ends: 30-Sep-2025
19,800.00
您節省 3,960.00 (20%)
Each
有庫存
新增至購物車
Quantity:
10 vials x 50 μg
Excitation/Emission:
∼396/610 nm
Indicator:
MitoSox Red
Color:
Red
價格 (TWD)
15,840.00
Online offer
Ends: 30-Sep-2025
19,800.00
您節省 3,960.00 (20%)
Each
新增至購物車
Ask our AI about this Product
MitoSOX Green and MitoSOX Red superoxide indicators are novel fluorogenic dyes specifically targeted to mitochondria in live cells. Oxidation of the MitoSOX reagent by mitochondrial superoxide produces bright green or red fluorescence.

Features of these indicators include:
• Readily oxidized by superoxide but not by other ROS- or RNS-generating systems
• Use for live cell imaging
• Rapidly and selectively targeted to the mitochondria
• MitoSOX Green indicator: absorption/emission maxima of ∼488/510 nm (traditional FITC/GFP filter set)
• MitoSOX Red indicator: absorption/emission maxima of ∼396/610 nm* (custom filter set recommended)

*Note that two excitation peaks may be observed for MitoSOX Red indicator, as it is excited at both 510 nm and 396 nm. While 510 nm will excite the superoxide oxidation product, it can also excite non-specific products, so we recommend using a 396-nm excitation for more selective detection of mito superoxide when using MitoSOX Red indicator (1).

MitoSOX Green indicator is offered as one vial or a pack of five vials. MitoSOX Red indicator is offered as one vial or a pack of ten vials. A variety pack of one vial each of MitoSOX Red and MitSOX Green indicators is also available.

Consult user Manual for solubility instructions.

Quick, easy detection of mitochondrial superoxide in live cells
The production of superoxide by mitochondria can be visualized in fluorescence microscopy using MitoSOX superoxide indicators. They permeate live cells where they selectively target mitochondria. They are rapidly oxidized by superoxide but not by other reactive oxygen species (ROS) and reactive nitrogen species (RNS). The oxidized product is highly fluorescent.

MitoSOX indicators may be used to distinguish artifacts of isolated mitochondrial preparations from direct measurements of superoxide generated in the mitochondria of live cells. They may also provide a valuable tool in the research of agents that modulate oxidative stress in various pathologies. In addition, these indicators have been used in metabolic flux assays using high-content instruments (2). MitoSOX indicators have also been used to detect mitochondrial superoxide using flow cytometery (3).

References
1: Robinson, Kristine M., et al. Selective fluorescent imaging of superoxide in vivo using ethidium-based probes. Proceedings of the National Academy of Sciences 103.41 (2006): 15038-15043.
2: Little, Andrew Charles, et al. High-content fluorescence imaging with the metabolic flux assay reveals insights into mitochondrial properties and functions. Communications biology 3.1 (2020): 1-10.
3: Kauffman, Megan E. et al. MitoSOX-Based Flow Cytometry for Detecting Mitochondrial ROS. Reactive oxygen species (Apex, N.C.) vol. 2,5 (2016): 361-370.
 

For Research Use Only. Not for use in diagnostic procedures.
規格
ColorRed
FormatVial(s)
Quantity10 vials x 50 μg
Detection MethodFluorescence
Excitation/Emission∼396/610 nm
IndicatorMitoSox Red
Product LineMitoSOX™
Unit SizeEach
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圖表

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證書

批號Certificate TypeDateCatalog Number(s)
3143139Certificate of AnalysisJun 01, 2025M36008
3179519Certificate of AnalysisMay 13, 2025M36007
3143101Certificate of AnalysisApr 09, 2025M36008
3006770Certificate of AnalysisOct 28, 2024M36008
2942298Certificate of AnalysisJul 23, 2024M36005
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安全資料表

常見問答集 (常見問題)

MitoSOX indicator-stained cells should be imaged within 2 hr and are not fixable.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

MitoSOX Mitochondrial Superoxide Indicators, for live-cell imaging are shipped at ambient temperature and should be stored as recommended.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The excitation wavelength for MitoSOX Red (Cat. Nos. M36008, M36007, M36009) was changed from 510 nm to 396 nm in the new manual because we have found that excitation at 396 nm selectively excites the superoxide oxidation product without exciting other non-specific products.  Although the indicator displays an adequate signal when excited at 510 nm, we highly recommend using the 396 nm excitation wavelength for selective detection of the indicator’s response to mitochondrial superoxide. For more information on the selective detection of superoxide at 396 nm, please refer to the article linked below.

Robinson KM, Janes MS, Pehar M, Monette JS, Ross MF, Hagen TM, Murphy MP, Beckman JS. Selective fluorescent imaging of superoxide in vivo using ethidium-based probes. Proc Natl Acad Sci U S A. 2006 Oct 10;103(41):15038-43. doi: 10.1073/pnas.0601945103. Epub 2006 Oct 2. PMID: 17015830; PMCID: PMC1586181.

The optimal emission wavelength to observe the superoxide oxidation product is 580 nm. Please note that the 610 nm wavelength mentioned in the MitoSOX Green and MitoSOX Red Mitochondrial Superoxide Indicators User Guide is incorrect.

We suggest a working concentration range of 100 nM to 5 µM.  It is recommended to optimize the final working concentration for each sample and application. It is important to be cautious when using concentrations >1 µM, as higher non-specific labeling may occur as well as possible mitochondrial toxicity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

No. We do not recommend storing MitoSOX Mitochondrial Superoxide Indicators (Cat. Nos. M36005, M3006, M36007, M36008, M36009) stock solutions beyond one day as they are more susceptible to spontaneous oxidation (from radicals in the atmosphere) when in solution compared to storing the reagents in solid form.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用資料與參考文獻 (104)

引用資料與參考文獻
Abstract
Superoxide dismutase mimetics: synthesis and structure-activity relationship study of MnTBAP analogues.
Authors:Gauuan PJ, Trova MP, Gregor-Boros L, Bocckino SB, Crapo JD, Day BJ
Journal:Bioorg Med Chem
PubMed ID:12110324
Carboxylic ester and amide-substituted analogues of [5,10,15,20-tetrakis(4-carboxyphenyl)-porphyrinato]manganese(III) chloride (MnTBAP) were synthesized and assayed as potential superoxide dismutase (SOD) mimetics. The tetraester analogues 4a and 4b were found to have comparable SOD activity to the known SOD mimetic MnTBAP, while amides 4c-4e exhibited reduced SOD activity. In the substituted methyl benzoate/acid ... More
Loss of PINK1 function promotes mitophagy through effects on oxidative stress and mitochondrial fission.
Authors:Dagda RK, Cherra SJ, Kulich SM, Tandon A, Park D, Chu CT,
Journal:J Biol Chem
PubMed ID:19279012
'Mitochondrial dysregulation is strongly implicated in Parkinson disease. Mutations in PTEN-induced kinase 1 (PINK1) are associated with familial parkinsonism and neuropsychiatric disorders. Although overexpressed PINK1 is neuroprotective, less is known about neuronal responses to loss of PINK1 function. We found that stable knockdown of PINK1 induced mitochondrial fragmentation and autophagy ... More
Imaging and analysis of 3D tumor spheroids enriched for a cancer stem cell phenotype.
Authors:Robertson FM, Ogasawara MA, Ye Z, Chu K, Pickei R, Debeb BG, Woodward WA, Hittelman WN, Cristofanilli M, Barsky SH,
Journal:J Biomol Screen
PubMed ID:20639504
'Tumors that display a highly metastatic phenotype contain subpopulations of cells that display characteristics similar to embryonic stem cells. These cells exhibit the ability to undergo self-renewal; slowly replicate to retain a nucleoside analog label, leading to their definition as ' ... More
Subcellular localization of Nox4 and regulation in diabetes.
Authors:Block K, Gorin Y, Abboud HE,
Journal:Proc Natl Acad Sci U S A
PubMed ID:19706525
'Oxidative stress is implicated in human diseases. Some of the oxidative pathways are harbored in the mitochondria. NAD(P)H oxidases have been identified not only in phagocytic but also in somatic cells. Nox4 is the most ubiquitous of these oxidases and is a major source of reactive oxygen species (ROS) in ... More
'Mild Uncoupling' does not decrease mitochondrial superoxide levels in cultured cerebellar granule neurons but decreases spare respiratory capacity and increases toxicity to glutamate and oxidative stress.
Authors:Johnson-Cadwell LI, Jekabsons MB, Wang A, Polster BM, Nicholls DG
Journal:J Neurochem
PubMed ID:17437552
'Cultured rat cerebellar granule neurons were incubated with low nanomolar concentrations of the protonophore carbonylcyanide-p-trifluoromethoxyphenyl hydrazone (FCCP) to test the hypothesis that ''mild uncoupling'' could be neuroprotective by decreasing oxidative stress. To quantify the uncoupling, respiration and mitochondrial membrane potential (Deltapsi(m)) were determined in parallel as a function of FCCP ... More
104 total citations

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