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Whole Allergen

t73 Australian pine Pollen

t73 Australian pine Pollen Scientific Information

Type:

Whole Allergen

Display Name:

Australian pine Pollen

Route of Exposure:

Inhalation

Family:

Casuarinaceae

Species:

Casuarina equisetifolia

Latin Name:

Casuarina equisetifolia

Other Names:

Coast ironwood, Beefwood, She-oak, Whistling-pine, Horsetail tree.

Summary

Australian pine (Casuarina equisetifolia) is a wind pollinated, drought tolerant tree with rapid growth, attaining a height ranging between 8 – 35 m. Its vegetation is usually seen in sub-tropical coastal areas, near the sea level. Its flowering season occurs from February through May. This tree is spread across more than 60 countries like China, Africa, Malaysia, the US, Europe, etc. Exposure to Australian pine pollen may induce allergic reactions such as allergic rhinitis, asthma, and atopic dermatitis in sensitized individuals. A study has identified a 17 kDa allergenic protein from Australian pine pollen, homologous to Bet v 1 (PR-10) birch allergen. Australian pine pollen has exhibited cross-reactivity with pollen from species of Betulaceae and Fagaceae family and unrelated species such as Quercus and Alnus.

Allergen

Nature

Australian pine (Casuarina equisetifolia) is a fast-growing tree, and is planted widely for wood production, erosion control, and production of timber, tannin, dye, and pulp (1). The height of the tree usually varies between 8–35 m.  (2). The bark of the tree is light-gray brown; the younger trees have smooth bark while older trees have rough and deeply furrowed bark (3). The tree crown is slender, finely branched, furrowed with greyish green branchlets (15–38 cm long and <1.0–1.5 mm in diameter). They have 7-8 dentate concentric circles (whorls) per node. Usually, male and female tress are separate. However, in some areas monoecious trees (male and female flowers on same tree) are also found. The spike inflorescence is observed in these trees with male inflorescences as simple, 7-40 mm long and elongated spikes and bears 7–11.5 whorls per cm of a spike. Female inflorescences are cone-shaped, 5–10 mm long and ellipsoid (2). Casuarina species are wind pollinated. The pollination occurs from February through May in the Northern Hemisphere (4).

Habitat

Australian pine trees are commonly found on sand dunes, estuaries behind foredunes, the leading edge of dune vegetation, and gentle slopes near the sea. This tree can be seen along thin belts near to sandy coastlines and up to an altitude of 100 m above sea level. They appear as crooked low-branching trees on exposed seashores and grow as straight-stemmed forests tree in sheltered areas. Well-drained, coarse textured, sands of 2 m or more in-depth soil type is usually preferred by this tree. It tolerates calcareous and slightly alkaline soils, and intolerant to waterlogging. (2). This tree is draught tolerant and can sustain without water for around 6 – 8 months (3). 

Taxonomy

Australian pine belonged to the genus Casuarina. This genus comprises about 14 species; of which 10 species were introduced outside their native territory. Only three species (C. equisetifolia, C. cunninghamiana, and C. glauca) are considered as naturalized or invasive. Species belonging to the genus Casuarina are usually called casuarians (such as Australian pine, beefwoods, she-oaks, etc.) (5).

Taxonomic tree of  Australian pine (6)
Domain Eukaryota
Kingdom Plantae
Phylum Spermatophyta
Subphylum Angiospermae
Class Dicotyledonae 
Order  Fagales
Family Casuarinaceae
Genus Casuarina
Species Casuarina equisetifolia
Taxonomic tree of  Australian pine (6)

Tissue

Australian pine pollen grains are medium sized from 25.25 x 27.82 μm. Its shape varies from oblate to spheroidal. They are having 3 or 4 apertures which are regularly spaced around the edge. The exine of the pollen grain is completely smooth (7).

Epidemiology

Worldwide distribution

Surveys have shown Australian pine (Casuarina equisetifolian of order Fagles) pollens triggering allergic reactions, particularly in the northern hemisphere's tropical areas (8)

Njokuocha and Osayi (2005) conducted a study in Nsukka (Nigeria) to identify allergic airborne particles in the atmosphere. Out of 2676 total pollen grains counted, 30 plant families and 33 pollen types were identified. Among these 33 types of pollen, 10 belonged to generic level and 23 to specific level. The study observed that pollens and fungal spores dominated the allergenic particles. Moreover, Australian pine pollen was found to be one of the most important causes of allergic rhinitis (AR) (9).

Mandal et al (2008) conducted a study in Calcutta, India, to identify the frequency of airborne pollen and the taxa that cause significant sensitization. Skin prick tests (SPT) were performed for the most common pollen types in patients with pollinosis conditions. Casuarina contributed around 5.76%, among other pollen taxa (10).

Three hundred eighty children aged 9 to 14 years, with (n =97) or without (n=107) asthma, participated in a study in Brisbane, Australia. The study analyzed SPT results to 15 commercial allergens. Among asthmatic children, 22% showed a positive response to Australian pine (11). 

Environmental Characteristics

Worldwide distribution

Australian pine is distributed widely in southeastern China, India, and Australia (12). More than 60 countries across Asia, Africa, America, Europe, and Oceania have it planted in their areas (1). It is distributed widely throughout subtropical and tropical coastlines in Australia, southern Thailand, Malaysia, the Philippines, the Pacific, Indonesia, Melanesia, Polynesia, and many other Middle Eastern countries. It was introduced into mostly tropical and warm subtropical countries, where it naturalized (2).

Route of Exposure

Main

The main route of exposure to this pollen is through inhalation (13).

Clinical Relevance

Several potent airborne pollen grains acting as bio-pollutants may trigger or enhance various respiratory and cutaneous allergic diseases such as bronchial asthma, bronchitis, naso-bronchial allergy, AR, conjunctivitis, dermatitis, and upper respiratory tract infection in sensitive individuals. Australian pine is one such source that produces aeroallergens that may be responsible for inducing respiratory symptoms and skin allergies (13).

Allergic rhinitis

A study compared the relationship between the pollen counts and documented allergy-related cases in Nigeria. The pollen counts were found to influence allergy (43.08%), upper respiratory tract infection (21.84%), AR (19.51%), and bronchitis (18.11%). Moreover, a significant strong positive correlation between the pollen of Australian pine and AR was observed (13).

Asthma

A study conducted among 142 patients (3 to 55 years old) with AR and asthma in Mexico City analyzed the frequency of sensitization to Australian pine pollen. Among 142 patients, 44 were children and 98 adults. The study concluded that eight children (18.18%) and 35 adults (35.7%) had a positive SPT to Australian pine pollen (14).

Atopic Dermatitis (AD)

A recent study found that 30% of the population is suffering from one or more allergic diseases such as bronchial asthma, AR, and AD that are increasing worldwide, including India. All India Coordinated Project on Aeroallergens and Human Health discovered the quantitative and qualitative prevalence of aerosols at 18 different country centers. Among other airborne pollen, Australian pine was predominantly present (15). 

Prevention and Therapy

Prevention strategies

Avoidance

One of the main measures to be taken is to cut down the amounts of pollen entering the respiratory system. During pollen season, allergic patients are suggested to stay indoors or wear masks outdoors to keep away from pollen allergens. In worse situations patients may be suggested to move to a non-pollen area (16).

Molecular Aspects

Allergenic molecules

An allergen identification study in Nigeria analyzed sera from 50 hypersensitive individuals and recognized a PR-10 protein of molecular weight (MW) 17kDa in Australian pine pollen. The study observed IgE-reactivity of 54% (27/50) for this allergenic protein. This PR-10 allergen is found to be homologous to Bet v 1, a major allergen from birch pollen (Betula verrucose) (8).

Cross-reactivity

Studies have shown strong cross-reactivity between Betulaceae and Fagaceae (17). Also, a study has shown that children sensitive to tree pollen gave positive responses to skin tests due to cross-reactions from different families, that is, Quercus, Alnus, and Casuarina. The study observed positive reactions even during low pollen concentrations (18).

Compiled By

Author: Turacoz Healthcare Solutions

Reviewer: Dr. Fabio Iachetti

 

Last reviewed: January 2021

References
  1. Muslim A. Australian pine cones-based activated carbon for adsorption of copper in aqueous solution. Journal of engineering science and technology. 2017;12(2):280-95.
  2. Pinyopusarerk K, Kalinganire A, Williams E, Aken KM. Evaluation of international provenance trials of Casuarina equisetifolia. Australia: Australian Centre for International Agricultural Research, 2004  Contract No.: 58e.
  3. Whistler W, Elevitch C. Casuarina equisetifolia (beach she-oak) C. cunninghamiana (river she-oak). Traditional Trees of Pacific Islands: Their Culture, Environment, and Use. 2006;227.
  4. Bucholtz G, Hensel 3rd A, Lockey R, Serbousek D, Wunderlin R. Australian pine (Casuarina equisetifolia) pollen as an aeroallergen. Annals of allergy. 1987;59(1):52-6.
  5. Potgieter LJ, Richardson DM, Wilson JR. Casuarina: biogeography and ecology of an important tree genus in a changing world. Biological Invasions. 2014;16(3):609-33.
  6. NCBI. Casuarina equisetifolia 2020 [December 30, 2020]. Available from: https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=3523&lvl=3&p=has_linkout&p=blast_url&p=genome_blast&lin=f&keep=1&srchmode=1&unlock.
  7. Chaurasia S. Pollen morphotypes of some common tree flora of Allahabad. International Journal of Multidisciplinary Research and Development. 2017;4(7):151-7.
  8. Adeniyi TA, Adeonipekun PA, Olowokudejo JD, Akande I. Allergenicity of dominant aeropollen in Nigeria (part II). Current Allergy & Clinical Immunology. 2018;31(3):178-83.
  9. Njokuocha R, Osayi E. Airborne pollen and spore survey in relation to allergy and plant pathogens in Nsukka, Nigeria. Bio-Research. 2005;3(1):77-84.
  10. Mandal J, Chakraborty P, Roy I, Chatterjee S, Gupta-Bhattacharya S. Prevalence of allergenic pollen grains in the aerosol of the city of Calcutta, India: a two year study. Aerobiologia. 2008;24(3):151-64.
  11. Gibbs JE. Eucalyptus pollen allergy and asthma in children: a cross-sectional study in South-East Queensland, Australia. PLoS One. 2015;10(5):e0126506.
  12. Weber R. On the cover. Australian pine. Annals of allergy, asthma & immunology: official publication of the American College of Allergy, Asthma, & Immunology. 2008;100(5):A4-A.
  13. Ige O, Essien B. Comparative Study of Dominant Aeropollen and Allergy-Related Cases in Akoko Environment, Ondo State, Nigeria. dermatitis. 2019;1(2):1-8.
  14. Velasco-Medina AA, Velázquez-Sámano G. Sensitization to Casuarina equisetifolia and Pinus spp Pollen in Patients with Allergic Rhinitis and Asthma in Mexico City. Revista Alergia México. 2014;61(1):9-13.
  15. Singh AB, Kumar P. Aeroallergens in clinical practice of allergy in India. An overview. Annals of Agricultural and Environmental Medicine. 2003;10(2):131-6.
  16. Xie Z-J, Guan K, Yin J. Advances in the clinical and mechanism research of pollen induced seasonal allergic Asthma. American journal of clinical and experimental immunology. 2019;8(1):1.
  17. Niederberger V, Pauli G, Grönlundc H, Fröschla R, Rumpold H, Kraft D, et al. Recombinant birch pollen allergens (rBet v 1 and rBet v 2) contain most of the IgE epitopes present in birch, alder, hornbeam, hazel, and oak pollen: a quantitative IgE inhibition study with sera from different populations. Journal of allergy and clinical immunology. 1998;102(4):579-91.
  18. Calderon-Ezquerro M, Guerrero-Guerra C, Galán C, Serrano-Silva N, Guidos-Fogelbach G, Jiménez-Martínez M, et al. Pollen in the atmosphere of Mexico City and its impact on the health of the pediatric population. Atmospheric Environment. 2018;186:198-208.