Cell Culture Reagents, Antibiotics and Supplements

Penicillin-Streptomycin (10,000 U/mL) Gibco™

This solution contains 10,000 units/mL of penicillin and 10,000 μg/mL of streptomycin. The antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria.

Antibiotic-Antimycotic (100X) Gibco™

Gibco™ Antibiotic-Antimycotic is used to prevent bacterial and fungal contamination. This solution contains 10,000 units/mL of penicillin, 10,000 μg/mL of streptomycin, and 25 μg/mL of Gibco Amphotericin B.

Penicillin-Streptomycin-Glutamine (100X) Gibco™

The antibiotics penicillin and streptomycin are used to prevent bacterial contamination in cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the...

Trypsin (0.25%), phenol red Gibco™

Gibco™ Trypsin solutions are made from trypsin powder—an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, it is widely used for cell dissociation, during routine cell culture passaging and primary tissue dissociation.

Poly-D-Lysine Gibco™

Poly-D-Lysine is a chemically synthesized extracellular matrix used to facilitate cell adhesion to tissue culture-treated plastic and glass surfaces. Poly-D-Lysine contains polymers with a molecular weight of 50,000–150,000 Daltons, which makes this product ideal for neuronal culture applications.

GlutaMAX™ Supplement Gibco™

Gibco GlutaMAX Supplement is an alternative to L-glutamine, with increased stability that improves cell health. GlutaMAX Supplement is suitable for both adherent and suspension culture of mammalian cells, with no adaptation required.

Anti-Clumping Agent Gibco™

GIBCO™ Anti-Clumping Agent is a concentrated liquid reagent that reduces cell clumping to assist in attaining higher viable cell densities in suspension culture. Anti-Clumping Agent is a gentle, animal origin-free, chemically defined formulation that contains no proteins, enzymes, hydrolysates, or...

2-Mercaptoethanol Gibco™

Gibco™ 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture media to prevent toxic levels of oxygen radicals. 2-Mercaptoethanol is not stable in solution, so most protocols require daily supplementation.

Collagenase, Type IV, powder Gibco™

Collagenase is a protease that cleaves the bond between a neutral amino acid (X) and glycine in the sequence Pro-X-Glyc-Pro, which is found with high frequency in collagen. Collagenase is unique among proteases in its ability to degrade the triple-helical native collagen fibrils commonly found in...

Hygromycin B (50 mg/mL) Gibco™

Hygromycin B is a water-soluble antibiotic purified from the bacterium Streptomyces hydroscopicus. Gibco™ Hygromycin B is used as a bacterial selection antibiotic in the concentration range of 200–500 μg/mL. This product is supplied as a 50 mg/mL solution.

Sodium Pyruvate (100 mM) Gibco™

Sodium pyruvate is commonly added to cell culture media as a carbon source in addition to glucose. Since cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not a required supplement for all cell cultures.

Versene Solution Gibco™

Versene is an EDTA solution for use as a gentle non-enzymatic cell dissociation reagent. Gibco Versene Solution (0.48 mM) is formulated as 0.2 g EDTA(Na4) per liter of Phosphate Buffered Saline (PBS). Product Use For Research Use Only. Not for use in diagnostic procedures.

StemPro™ Accutase™ Cell Dissociation Reagent Gibco™

StemPro® Accutase® Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes. Accutase® does not contain mammalian or bacterial derived products and is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated...

MEM Vitamin Solution (100X) Gibco™

Gibco™ MEM Vitamin Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco™ MEM Vitamin Solution is formulated to contain 100x the vitamins found in the standard Minimum Essential Medium (MEM). The complete formulation is available.

HT Supplement (100X) Gibco™

Gibco HT Supplement is a liquid mixture of sodium hypoxanthine (10 mM) and thymidine (1.6 mM). HT-supplemented medium is suitable for post-selection rescue to overcome the effects of residual intracellular aminopterin.