TaqMan probes and qPCR primers are an optimal choice for real-time PCR research experiments that cannot be addressed with Thermo Fisher Scientific predesigned, ready-to-use TaqMan assays. Researchers can select from a collection of dual-labeled TaqMan probes and unlabeled oligos for qPCR primers, providing the flexibility to meet various experimental needs. Whether researchers target multiple sequences simultaneously, using custom probes via bioinformatics, or detecting exotic targets that do not already have a predesigned assay, Thermo Fisher Scientific offers excellent probes for designing custom assays. The TaqMan MGB (minor groove binder), TAMRA, QSY, and QSY2 custom probes deliver exceptional performance. Manufactured with the same facilities and raw materials as the TaqMan assays featured in over 296,000 publications, these custom qPCR probes and primers help ensure quality and reliability.
TaqMan probes are highly reliable and widely used in various research applications, particularly in quantitative PCR (qPCR) experiments. These customizable probes are designed to specifically bind to the target sequence, allowing for precise and sensitive detection of the desired nucleic acid. TaqMan probes utilize a dual-labeled format, with a fluorophore at the 5' end and a quencher at the 3' end. During amplification, the probe is cleaved by the DNA polymerase, resulting in the separation of the fluorophore and quencher, leading to a fluorescent signal. This signal is directly proportional to the amount of target nucleic acid present in the sample. TaqMan probes are known for their exceptional quality, reproducibility, and widely used chemistry, making them a trusted choice for researchers seeking accurate and reliable results in their qPCR experiments.
See how TaqMan probes can save you time and reduce costs with high-quality data and advanced multiplexing. Check out our head-to-head comparison with Supplier I in singleplex and multiplex qPCR assays.
A quencher is a molecule that suppresses the fluorescence of a fluorophore. This suppression, known as quenching, can occur through various mechanisms and is critical for many fluorescence-based assays and probes, including those used in genetic analysis and molecular diagnostics. Learn more about real-time PCR from our Taq Talk Video Series
qPCR primers are essential tools in the field of molecular biology, designed to amplify specific DNA sequences with high precision and efficiency. Sequence detection primers are custom primers for real-time PCR that can be used with either TaqMan probes or SYBR dye, making them versatile for real-time PCR research applications. Tailor-made to match the unique requirements of each experimental setup, these custom primers help ensure accurate target amplification, thereby enhancing the sensitivity and specificity of the assay for more reliable and reproducible data. Additionally, these qPCR primers are desalted, available in various scales, and can be shipped either as liquid or dried down, providing flexibility and convenience for researchers. Custom qPCR primers allow for optimal performance in diverse applications, including gene expression analysis, genotyping, and pathogen detection.
Set your assays up for success—the quality of the qPCR data is defined by the quality of the custom qPCR probes and primers chosen
HPLC-purified probes—Removing impurities such as unconjugated dyes or truncated probes that can increase background signal and decrease assay sensitivity
Option to ship in solution—Avoid errors during resuspension that can alter stock concentrations and markedly affect downstream experiments
Guaranteed yield—Ensures the right amount of qPCR assays for every sample size and scale
Efficient integration—TaqMan probes are designed for seamless integration into the Applied Biosystems workflow, including latest master mixes
Competitive shipping—Similar turnaround times in North America as compared to competitors*
*Estimated 4–10 days in North America. International times may vary.
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Streptococcus suis manganese transporter mutant as a live attenuated vaccine: Safety, efficacy, and virulence reversion mechanisms.
"Abstract: Streptococcus suis is the leading cause of mortality in piglets and is responsible for severe economic losses in the global pork industry. Severe invasive diseases caused by S. suis include sepsis, meningitis, arthritis, and endocarditis""Abstract: Streptococcus suis is the leading cause of mortality in piglets and is responsible for severe economic losses in the global pork industry. Severe invasive diseases caused by S. suis include sepsis, meningitis, arthritis, and endocarditis. S. suis disease prevention is hampered by the lack of safe and efficacious vaccines"More...
Establishment and application of a qPCR method for differential detection of Brucella S2 vaccine strain.
"04):37–40 in Chinese. 28. Zhuo XJ. Immune measures of bovine and sheep brucellosis and its application prospect. Anim Ind Environ. 2024;08:70–2 in Chinese. 29. Gao ZQ, Xing J, Feng YF, Yue BF, He ZM. TaqManMGB probe real-time PCR for rapid detection of Brucella. Chin J Zoonoses. 2011;27(11):995–1000 in Chinese. 30. Liu HW, Xu L, Fan Y, Bao GY, Chen JJ, Zhang TC. Study on the detection of Brucella ""on of sheep brucellosis. Xinjiang Xumuye. 2024;40(04):37–40 in Chinese. 28. Zhuo XJ. Immune measures of bovine and sheep brucellosis and its application prospect. Anim Ind Environ. 2024;08:70–2 in Chinese. 29. Gao ZQ, Xing J, Feng YF, Yue BF, He ZM. TaqManMGB probe real-time PCR for rapid detection of Brucella. Chin J Zoonoses. 2011;27(11):995–1000 in Chinese. 30. Liu HW, Xu L, Fan Y, Bao GY, Chen JJ, Zhang TC. Study on the detection of Brucella nucleic acid in blood of patients with brucellosis"More...
The impact of ingestion of Bifidobacterium longum NCC3001 on perinatal anxiety and depressive symptoms: a randomized controlled trial
"e QIAamp FAST DNA Stool Mini Kit (51604, Qiagen). DNA concentrations were measured using the PicoGreen fluorescence method (Thermo Fisher). The abundance of the BL NCC3001 strain was detected using a TaqManMGB assay (Primer Express 3.0, Applied Biosystems) targeting a strain-specific chromosomic region that has been identified in previous studies 25 , 36 . The MasterMix LightCycler ® Multiplex DNA""en fluorescence method (Thermo Fisher). The abundance of the BL NCC3001 strain was detected using a TaqManMGB assay (Primer Express 3.0, Applied Biosystems) targeting a strain-specific chromosomic region that has been identified in previous studies 25 , 36 . The MasterMix LightCycler ® Multiplex DNA Master (07339585001, Roche) was used with a final concentration of 0.9 µM for each primer and 0.25 µM of the probe. Each data point was run as technical triplicates and a standard curve was built in serial 10-fold dilution of BL NCC3001 genomic DNA. The assay was performed on a LC480 II cycler (Roche) using the following PCR conditions: 7 min at 95 °C for Taq activation, 10 s at 95 °C for denaturation and 30 s at 60 °C for annealing and extension x 40 cycles then the cooling 30 s at 40 °C. Another TaqManMGB assay was used to normalize the BL NCC3001 abundance relative to the bacterial load (tota"More...
Characterization of RNA Helicase Genes in Ustilago maydis Reveals Links to Stress Response and Teliospore Dormancy.
International journal of molecular sciences | 2025 Mar 27 | PubMed ID: 40141077 | Read Article
"s separated by agarose gel electrophoresis. Agarose gels contained 0.3 µg/mL of ethidium bromide (BioShop Canada, Burlington, ON, Canada), and PCR products were visualized under UV light. Primers and TaqManMGB probes for RT-qPCR were designed with Primer Express Software version 2.0 (Thermo Fisher Scientific, Mississauga, ON, Canada) using default criteria for genes UMAG_00175 and uded1 (Table S2)""by a 4 ◦C hold. One-third of the RT-PCR product was separated by agarose gel electrophoresis. Agarose gels contained 0.3 µg/mL of ethidium bromide (BioShop Canada, Burlington, ON, Canada), and PCR products were visualized under UV light. Primers and TaqManMGB probes for RT-qPCR were designed with Primer Express Software version 2.0 (Thermo Fisher Scientific, Mississauga, ON, Canada) using default criteria for genes UMAG_00175 and uded1 (Table S2). RT-qPCR reactions were carried out with 2.0 µL o"More...
Methods and kits useful for diagnosis of human papillomavirus (HPV)
"Master Mix (2λ) and custom TaqMan™ Assay used in PCR was supplied by Thermofisher Scientific. The custom TaqMan™ Assay was composed as followings: Forward primer, Reverse primer and a FAM dye-labeled TaqManMGB probe.
Plasma Cell Lysis
Each 2 mL of human pooled plasma was spiked with 100 fg 145 bp DNA+1.5 uL 2000 bp DNA (TATAA) stock. 160 uL of suitable lysis buffer and 60 uL of Proteinase K (28.5 ""s were of analytical grade.
TaqMan™ Fast Advanced Master Mix (2λ) and custom TaqMan™ Assay used in PCR was supplied by Thermofisher Scientific. The custom TaqMan™ Assay was composed as followings: Forward primer, Reverse primer and a FAM dye-labeled TaqManMGB probe.
Plasma Cell Lysis
Each 2 mL of human pooled plasma was spiked with 100 fg 145 bp DNA+1.5 uL 2000 bp DNA (TATAA) stock. 160 uL of suitable lysis buffer and 60 uL of Proteinase K (28.5 mg/mL) was added into each 2 mL spiked plasma. The"More...
Statewide surveillance of tick-borne pathogens in ticks collected in Delaware using novel multiplex PCR assays
International Journal for Parasitology: Parasites and Wildlife | 2025 Mar 21 | Read Article
" they normally require a longer sequence than other probe types such as MGB. This is due in part to the general requirement of QSY probe melting temperature of 70 °C. In addition, the primers used in TaqmanQSY real-time PCR require primer sets to have a melting temperature, at, or close to 60 °C. These relatively stringent parameters sometimes make primer and probe development difficult. However, ""sely ( Bonab ). One drawback of QSY probes is that they normally require a longer sequence than other probe types such as MGB. This is due in part to the general requirement of QSY probe melting temperature of 70 °C. In addition, the primers used in TaqmanQSY real-time PCR require primer sets to have a melting temperature, at, or close to 60 °C. These relatively stringent parameters sometimes make primer and probe development difficult. However, if sequences are found that are compatible, then s"More...
Novel mechanism for tubular injury in nephropathic cystinosis
"eaction (qPCR). For qPCR, we designed two primers targeting two specific exons on CTNS gene – primer#1 targets between exon 2–3 and primer#2 targets between exon 9–10. These primers were connected to TaqmanMGB probe (Thermo Fisher, Waltham, MA). We diluted the cDNA and used 1.25 ng for the qPCR reaction. Briefly, we added master mix (Applied Bioscience, Waltham, MA) that contains all the reaction "" used next day for quantitative Polymerase Chain Reaction (qPCR). For qPCR, we designed two primers targeting two specific exons on CTNS gene – primer#1 targets between exon 2–3 and primer#2 targets between exon 9–10. These primers were connected to TaqmanMGB probe (Thermo Fisher, Waltham, MA). We diluted the cDNA and used 1.25 ng for the qPCR reaction. Briefly, we added master mix (Applied Bioscience, Waltham, MA) that contains all the reaction components, nuclease-free water, and primers to th"More...
Novel Mechanism for Tubular Injury in Nephropathic Cystinosis
"eaction (qPCR). For qPCR, we designed two primers targeting two specific exons on CTNS gene – primer#1 targets between exon 2–3 and primer#2 targets between exon 9–10. These primers were connected to TaqmanMGB probe (Thermo Fisher, Waltham, MA). We diluted the cDNA and used 1.25 ng for the qPCR reaction. Briefly, we added master mix (Applied Bioscience, Waltham, MA) that contains all the reaction "" used next day for quantitative Polymerase Chain Reaction (qPCR). For qPCR, we designed two primers targeting two specific exons on CTNS gene – primer#1 targets between exon 2–3 and primer#2 targets between exon 9–10. These primers were connected to TaqmanMGB probe (Thermo Fisher, Waltham, MA). We diluted the cDNA and used 1.25 ng for the qPCR reaction. Briefly, we added master mix (Applied Bioscience, Waltham, MA) that contains all the reaction components, nuclease- free water, and primers to t"More...
Sedimentary DNA is a promising indicator of the abundance of marine benthos: Insights from the burrowing decapod Upogebia major.
PloS one | 2025 Mar 19 | PubMed ID: 40106512 | Read Article
"ly related species U. yokoyai was explored and designated as the sequence for the speciesspecific primer-probe for U. major. The primer (forward primer: UMFW2208, Reverse primer: UMRV2208) and probe (TaqManMGB Probe: UMPb1) sequences are listed in Table 3. The PCR amplification size achieved through PCR was 94 bp.
In order to validate the species-specificity of the designed primer-probe set, quant""imer-probe for U. major. The primer (forward primer: UMFW2208, Reverse primer: UMRV2208) and probe (TaqManMGB Probe: UMPb1) sequences are listed in Table 3. The PCR amplification size achieved through PCR was 94 bp.
In order to validate the species-specificity of the designed primer-probe set, quantitative polymerase chain reactions (qPCR) were performed on genomic DNA extracted from the muscle tissues of U. major, its closely related species U. yokoyai, and the dominant species at each survey site, including Asari clam R. philippinarum, hermit crab Pagurus minutus and horn snail Batillaria attramentaria (refer to next paragraph for the composition of the qPCR reaction solution and PCR conditions). Only the DNA of U. major was amplified, substantiating its species specificity (S1 Fig).
Quantification of sedDNA concentration using qPCR The qPCR used in this study was performed using the StepOne Real-Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA). The total volume of the qPCR reaction mixture was 20 µL, which included 2 µL of DNA extraction solution, 10 µL of TaqMan
PLOS ONE | https://doi.org/10.1371/journal.pone.0318235 March 19, 2025 9 / 25
Fast Advanced Master Mix (Thermo Fisher Scientific, Waltham, MA, USA), 1.8 uL each of 10 pmol/uL primers (final concentration 900 nM), 0.5 µL of 10 pmol/µL TaqManMGB probe (250 nM), and 3.9 µL of nuclease-free water. qPCR was performed using a dilution se"More...
Sedimentary DNA is a promising indicator of the abundance of marine benthos: Insights from the burrowing decapod Upogebia major
" related species U. yokoyai was explored and designated as the sequence for the species-specific primer-probe for U. major . The primer (forward primer: UMFW2208, Reverse primer: UMRV2208) and probe (TaqManMGB Probe: UMPb1) sequences are listed in Table 3 . The PCR amplification size achieved through PCR was 94 bp. In order to validate the species-specificity of the designed primer-probe set, qua""common to all groups and distinct from the closely related species U. yokoyai was explored and designated as the sequence for the species-specific primer-probe for U. major . The primer (forward primer: UMFW2208, Reverse primer: UMRV2208) and probe (TaqManMGB Probe: UMPb1) sequences are listed in Table 3 . The PCR amplification size achieved through PCR was 94 bp. In order to validate the species-specificity of the designed primer-probe set, quantitative polymerase chain reactions (qPCR) were p"More...
Fabrication of 3D Biofunctional Magnetic Scaffolds by Combining Fused Deposition Modelling and Inkjet Printing of Superparamagnetic Iron Oxide Nanoparticles.
Tissue engineering and regenerative medicine | 2025 Mar 18 | PubMed ID: 40100619 | Read Article
"osystems, Thermo Fisher Scientific, Foster City, CA, USA). The expression levels of ALP and Runx2 were determined by qRT-PCR using the Taqman probes Hs01029144_m1 and Hs01047973_m1 (FAMTM dye-labeled TaqManMGB probes, Applied Biosystems). For quantification of gene expression, the target gene values were normalized to the expression of the endogenous reference GAPDH (Glyceraldehyde 3-phosphate deh"" using a high-capacity RNA-to-cDNA kit (Applied Biosystems, Thermo Fisher Scientific, Foster City, CA, USA). The expression levels of ALP and Runx2 were determined by qRT-PCR using the Taqman probes Hs01029144_m1 and Hs01047973_m1 (FAMTM dye-labeled TaqManMGB probes, Applied Biosystems). For quantification of gene expression, the target gene values were normalized to the expression of the endogenous reference GAPDH (Glyceraldehyde 3-phosphate dehydrogenase, Hs02786624_g1). The comparative thresh"More...
Sulforaphane-cysteine for wound healing in diabetic subjects
" Reverse Transcriptase Kit, Invitrogen) and amplified using the TaqMan Assay on Demand (Applied Biosystems) in a 25 μL reaction volume containing two unlabeled primers, a 6-carboxyfluorescien-labeled TaqManMGB probe and the master mix. The amplified sequences were assessed using the ABI 7500 Prism Sequence Detection system machine. The results were expressed as mRNA levels normalized to 18S or GAP"" μg of RNA was reverse transcribed (Superscript II Reverse Transcriptase Kit, Invitrogen) and amplified using the TaqMan Assay on Demand (Applied Biosystems) in a 25 μL reaction volume containing two unlabeled primers, a 6-carboxyfluorescien-labeled TaqManMGB probe and the master mix. The amplified sequences were assessed using the ABI 7500 Prism Sequence Detection system machine. The results were expressed as mRNA levels normalized to 18S or GAPDH in each sample.
Cell Transfection and Nrf2 Acti"More...
Regulation of serum reproductive hormones, gap junction proteins, and cytokine profiles in laying hens fed varying levels of expanded black soldier fly meal
"e gene (18S rRNA), was carried out in a 10 μL reaction volume run in duplicate. This mixture included 5 μL of TaqMan Gene Expression Master Mix, 0.5 μL of TaqMan Gene Expression Assay with a specific TaqManMGB probe and primers (provided by Applied Biosystems), 0.5 μL of Eukaryotic 18S rRNA Endogenous Control (consisting of primers and a VIC/TAMRA-labeled TaqMan probe), 3 μL of water, and 1 μL of ""R, targeting the gene of interest and the reference gene (18S rRNA), was carried out in a 10 μL reaction volume run in duplicate. This mixture included 5 μL of TaqMan Gene Expression Master Mix, 0.5 μL of TaqMan Gene Expression Assay with a specific TaqManMGB probe and primers (provided by Applied Biosystems), 0.5 μL of Eukaryotic 18S rRNA Endogenous Control (consisting of primers and a VIC/TAMRA-labeled TaqMan probe), 3 μL of water, and 1 μL of cDNA. A reaction without a DNA or cDNA template wa"More...
Mitochondrial mt12361A>G increased risk of metabolic dysfunction-associated steatotic liver disease among non-diabetes
World Journal of Gastroenterology | 2025 Mar 14 | Read Article
" protocols[ 39 - 41 ]. The Taqman ® allelic discrimination assay was used for genotyping the significant mtSNP in the validation dataset. The probe and primer sequences of mt12361A>G are as follows: TaqManMGB probes: VIC-CACTACTATAACCACCCTAAC (wild type); FAM-ACTACTATAACCGCCCTAAC (variant); Forward primer: 5’-GGTGCAACTCCAAATAAAAGTAATAACCA-3’; Reverse primer: 5’-GTGGATGCGACAATGGATTTTACAT-3’. Genot""as performed according to the previously published protocols[ 39 - 41 ]. The Taqman ® allelic discrimination assay was used for genotyping the significant mtSNP in the validation dataset. The probe and primer sequences of mt12361A>G are as follows: TaqManMGB probes: VIC-CACTACTATAACCACCCTAAC (wild type); FAM-ACTACTATAACCGCCCTAAC (variant); Forward primer: 5’-GGTGCAACTCCAAATAAAAGTAATAACCA-3’; Reverse primer: 5’-GTGGATGCGACAATGGATTTTACAT-3’. Genotyping was performed according to the manufacturer’"More...
Utilizing Functionalized Dual-Microbead Interfaces for Measurements of Amyloid-beta (1-42) with Femtomolar Sensitivity.
Langmuir : the ACS journal of surfaces and colloids | 2025 Mar 11 | PubMed ID: 39993947 | Read Article
"Abstract: The presence of amyloid-beta (1-42) in CSF and plasma has been implicated as a pathological hallmark of Alzheimer's disease. Here, we demonstrate a high-sensitivity method of detection and quantification for total Aβ42 utilizing two-antibody functionalized microparticles interfaces. Our method of biomarker detection utilizes two types of microbeads: (1) magnetic particles functionalized with monoclonal antibodies and (2) polystyrene particles dual functionalized with monoclonal antibodies and a unique DNA "tag"""Abstract: The presence of amyloid-beta (1-42) in CSF and plasma has been implicated as a pathological hallmark of Alzheimer's disease. Here, we demonstrate a high-sensitivity method of detection and quantification for total Aβ42 utilizing two-antibody functionalized microparticles interfaces. Our method of biomarker detection utilizes two types of microbeads: (1) magnetic particles functionalized with monoclonal antibodies and (2) polystyrene particles dual functionalized with monoclonal antibodies and a unique DNA "tag". The bead complex that forms in the presence of the target analyte is detected using qPCR to determine the analyte concentration. To demonstrate our method, we tested for the presence of amyloid-beta (1-42) in Tris buffer"More...
Using a novel gene site to develop a duplex real-time TaqMan MGB probe PCR method for the SNP detection and differentiation between the MS-H live vaccine strain and wild-type Mycoplasma synoviae strains
"al 21 candidate detection targets were selected by conservativeness and specificity analyses, and were using in subsequent assays. Based on the targets screened above, we developed a duplex real-time TaqManMGB probe PCR method utilizing a hydrolysis TaqMan oligonucleotide probe, specifically MGB probe. This probe loaded a reporter dye at the 5′-end and attached a NFQ quenching dyes at the 3′-end. ""re using in subsequent assays. Based on the targets screened above, we developed a duplex real-time TaqManMGB probe PCR method utilizing a hydrolysis TaqMan oligonucleotide probe, specifically MGB probe. This probe loaded a reporter dye at the 5′-end and attached a NFQ quenching dyes at the 3′-end. The inclusion of the NFQ in the MGB probe significantly reduces background fluorescence ( Kutyavin et al., 2000 ). Through this, the highly stable interaction between the MGB probe and the target increases the melting temperature (Tm) of the probe and its specificity ( Sylvain et al., 2004 ; Kutyavin et al., 2000 ). This method is applicable in both single and multiplex formats for SNP detection across many species ( Kutyavin, 2010 ; Tomás et al., 2012 ), including Helicobacter pylori ( Zhao et al., 2022 ), Mucormycosis ( Bergallo et al., 2022 ), and avian influenza viruses ( Zhang et al., 2017 ). In this study, a pair of primers and two specific MGB probes were designed for the SNP mutation site of the ktrB gene, one probe was specific for attenuated vaccine strain MS-H and the other probe was for wild-type MS, which could simultaneously identify MS-H and wild-type MS in one reaction. Orthogonal experiments were conducted to determine the optimal primer and probe concentrations. The optimized reaction system not only can accurately distinguish vaccine strain MS-H from wild-type MS, but also can be used for clinical detection of MS infection. The specificity test revealed no non-specific reactions with other avian pathogens, demonstrating high specificity. In conjunction with the results from the interference test, it was further confirmed that the method could accurately identify the target strains even in the presence of a large number of clinically interfering strains. In the sensitivity test, the detection limits for MS-H and wild-type MS were 6.25 copies/μL and 14.4 copies/μL, respectively. Additionally, the detection limits for the simulated clinical samples contaminated with MS-H strain and wild-type MS were 5.75 CFU/mL and 1.0 × 10 2 CFU/mL, respectively. The sensitivity of the TaqmanMGB method established using this ktrB gene exceeds that of existing methods. Dijkman ( Dijkm"More...
Characterization of RNA Helicase Genes in Ustilago maydis Reveals Links to Stress Response and Teliospore Dormancy
International Journal of Molecular Sciences | 2025 Mar 08 | Read Article
" separated by agarose gel electrophoresis. Agarose gels contained 0.3 µg/mL of ethidium bromide (BioShop Canada, Burlington, ON, Canada), and PCR products were visualized under UV light. Primers and TaqManMGB probes for RT-qPCR were designed with Primer Express Software version 2.0 (Thermo Fisher Scientific, Mississauga, ON, Canada) using default criteria for genes UMAG_00175 and uded1 ( Table S2""y a 4 °C hold. One-third of the RT-PCR product was separated by agarose gel electrophoresis. Agarose gels contained 0.3 µg/mL of ethidium bromide (BioShop Canada, Burlington, ON, Canada), and PCR products were visualized under UV light. Primers and TaqManMGB probes for RT-qPCR were designed with Primer Express Software version 2.0 (Thermo Fisher Scientific, Mississauga, ON, Canada) using default criteria for genes UMAG_00175 and uded1 ( Table S2 ). RT-qPCR reactions were carried out with 2.0 µL"More...
Using a novel gene site to develop a duplex real-time TaqMan MGB probe PCR method for the SNP detection and differentiation between the MS-H live vaccine strain and wild-type Mycoplasma synoviae strains.
"Abstract: Mycoplasma synoviae (MS) is a globally prevalent avian pathogen responsible for airsacculitis and synovitis. The temperature-sensitive (ts)+ vaccine strain MS-H, a live attenuated variant, is the most effective and widely used vaccine for controlling infections in the poultry industry. Consequently, accurate detection is essential for a strategy known as differentiating infected from vaccinated animals (DIVA)""Abstract: Mycoplasma synoviae (MS) is a globally prevalent avian pathogen responsible for airsacculitis and synovitis. The temperature-sensitive (ts)+ vaccine strain MS-H, a live attenuated variant, is the most effective and widely used vaccine for controlling infections in the poultry industry. Consequently, accurate detection is essential for a strategy known as differentiating infected from vaccinated animals (DIVA). In this study, we developed a duplex real-time TaqMan minor groove binder (MGB) probe PCR (The DRTM-probe PCR) method to differentiate the MS-H live vaccine strain from wild-type strains by targeting a single nucleotide polymorphism (SNP) in the ktrB gene. This gene overcomes the restoration of the genotype of wild-type 86079/7NS in specific regions"More...
Real‐Time PCR Assay and Environmental DNA Workflow for Detecting Irukandji Jellyfish, Malo bella (Cubozoa)
"and Dawson 2010) in place of M. bella primers was used for each species as a control. Gel electrophoresis confirmed the correct amplicon size.
Subsequent specificity testing via qPCR incorporated the TaqManMGB probe, using a QuantStudio 3 Real- Time PCR system (ThermoFisher Scientific Pty Ltd., Australia) (initial denaturation step of 95°C for 10 min, followed by 40 cycles of 95°C for 15 s and 60°"" and Schierwater 2003) and Aa_H16S_15141H (Bayha and Dawson 2010) in place of M. bella primers was used for each species as a control. Gel electrophoresis confirmed the correct amplicon size.
Subsequent specificity testing via qPCR incorporated the TaqManMGB probe, using a QuantStudio 3 Real- Time PCR system (ThermoFisher Scientific Pty Ltd., Australia) (initial denaturation step of 95°C for 10 min, followed by 40 cycles of 95°C for 15 s and 60°C for 1 min). Each 10 μL reaction, run in triplica"More...
Sample manipulation and assay with rapid temperature change
". Pat. No. 5,210,015, which is entirely incorporated herein by reference. Non-limiting examples of probes that can be useful in Q-PCR or RTQ-PCR reactions include TaqMan probes, TaqMan Tamara probes, TaqManMGB probes, or Lion probes.
A variety of arrangements of quencher and fluorescent dye can be used when both are used. In the case of a molecular beacon, for example, a quencher is linked to one ""ed quantitative amplification are described in U.S. Pat. No. 5,210,015, which is entirely incorporated herein by reference. Non-limiting examples of probes that can be useful in Q-PCR or RTQ-PCR reactions include TaqMan probes, TaqMan Tamara probes, TaqManMGB probes, or Lion probes.
A variety of arrangements of quencher and fluorescent dye can be used when both are used. In the case of a molecular beacon, for example, a quencher is linked to one end of an oligonucleotide capable of forming a hai"More...
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