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Predesigned Single Tube TaqMan Gene Expression Assays
Single-tube, predesigned gene expression assays offer a streamlined and efficient solution for real-time PCR analysis. These assays come pre-formulated in a single tube containing a labeled TaqMan MGB probe, along with forward and reverse primers. The single-tube format minimizes pipetting steps and accelerates the workflow, making it easier to scale throughput for high-volume studies. Designed to detect virtually any transcript, single-tube predesigned gene expression assays are available for over 30 species, including common microbial species, helping ensure broad applicability in various research fields. Additionally, the assays are shipped with either a 2D barcode laser-etched on the bottom of each assay tube or a 1D-barcoded 96-position racks, facilitating easy tracking and organization in the lab.
Ready to use single tube gene expression assays:
The TaqMan gene expression assay search tool below allows researchers to explore over 2.8 million meticulously designed gene expression assays and arrays, all supported by the TaqMan performance guarantee*.
Search all predesigned single tube TaqMan gene expression assays
Select from non-coding RNA, marker and reporter, fusion assays, or search all gene expression assays.
Product information for single-tube gene expression assays
Single-tube TaqMan gene expression assays are delivered in a single tube containing the specific pre-formulated TaqMan assay (TaqMan MGB probe and forward and reverse primers). The assays are shipped at ambient temperature and storage is recommended at –15 to –25ºC. For more product information about single tube gene expression assays, please refer to the product pages linked below:
TaqMan Gene Expression Assay, FAM
TaqMan Gene Expression Assay, VIC
TaqMan Gene Expression Assay, VIC primer-limited
Predesigned single-tube TaqMan gene expression specific assay types
TaqMan non-coding RNA gene expression assays
Reliably detect and quantitate non-coding transcripts longer than 200 nucleotides with TaqMan non-coding RNA gene expression assays. Used with a standard reverse transcription kit and a qPCR master mix, these assays facilitate the study of regulatory non-coding RNAs in mammalian and other complex genomes. An exclusive set of TaqMan gene expression assays targets long non-coding RNA transcripts, ensuring specificity even when coding transcripts are present at the same locus.
Search predesigned single tube TaqMan non-coding RNA assays with the tool at the top of the page. For more product information about single tube TaqMan non-Coding RNA gene expression assays, please refer to TaqMan Non-Coding RNA Assay
The benefits of these assays include:
- Highly specific—Detects only the intended non-coding RNA transcript.
- Fast, simple, and scalable—Two-step quantitative RT-PCR assay enables high-quality results in less than three hours.
- Verified design—Designed using the same verified pipeline and workflow as TaqMan Gene Expression Assays.
- Species coverage—Available for 12 different species.
TaqMan marker and reporter single-tube gene expression assays
TaqMan marker and reporter gene expression assays enable researchers to reliably detect and quantitate DNA or RNA sequences encoding common marker and reporter proteins, such as green fluorescent protein (GFP) and its derivatives. They are designed using the same verified pipeline as TaqMan gene expression assays. Together with a TaqMan master mix, these assays offer a fast, simple, and scalable technique for quantifying markers and reporters. Search predesigned single tube TaqMan marker and reporter assays with the tool at the top of the page.
For more product information about single-tube TaqMan marker and reporter gene expression assays, please refer to Single tube TaqMan marker and reporter gene expression assays
TaqMan Marker and Reporter Assays can be used to:
- Check for successful cloning and/or transfection of fluorescent fusion protein.
- Determine the efficacy of a cellular delivery system by quantifying the amount of plasmids delivered to the cell
- Quantify copy numbers of a transgene in cell lines
- Detect the SeV genome and transgenes in cells reprogrammed using the CTS CytoTune 2.0/2.1 Sendai reprogramming vectors
Marker and reporter gene expression assays available
TaqMan single-tube gene expression assays utilize TaqMan MGB probes, which include a reporter dye (such as FAM) at the 5' end and a non-fluorescent quencher (NFQ) at the 3' end. These probes are designed to increase the melting temperature (Tm) without increasing the probe length, allowing for the design of shorter probes and improving assay specificity.
TaqMan assays are highly sensitive and specific, capable of detecting low levels of expression or low-abundance targets and are ideal for measuring small fold-changes with high accuracy. They are widely used in gene expression analysis, including applications in reprogramming and stem cell research, where specific genes like SeV, KOS, Klf4, cMyc, L-Myc, Sox2, and Oct3/4 are commonly studied.
Expressed fluorescent protein reporters
- Enhanced GFP
- YFP
- GFP
- mCherry
- LacZ
- Luciferase
- AcGFP
- AmCyan
- DsRed-Monomer
- E2-Crimson
- HcRed1
- ZsYellow
- mBanana
- mOrange2
- mPlum
- mRasberry
- mStrawberry
- tdTomato
Reprogramming kit markers
- SeV
- KOS
- Klf4
- CMyc
- L-Myc
- Sox2
- Oct3/4
TaqMan fusion gene expression assays
This specialized set of TaqMan gene expression assays is designed to detect fusion transcripts using real-time PCR. Certain fusion transcripts are considered rare and are not well characterized; therefore, detecting these rare events using a single technology can deliver ambiguous results. TaqMan gene expression assays are a complementary solution to next-generation sequencing (NGS) panels, designed to serve as an orthogonal validation method for confirming NGS results. Search predesigned single tube TaqMan fusion assays with the tool at the top of the page.
- Built from proven TaqMan assay chemistry
- Performance guaranteed for all predesigned assays*
- Consists of a single tube, which contains a probe, forward primer, and reverse primer for each target for use in the simple, fast PCR workflow
- Use universal cycling conditions—run any combination of assays in parallel on a single real-time PCR instrument
*Terms and conditions apply
Documentation for TaqMan gene expression products
Documentation for TaqMan Gene Expression products, including the protocol, product insert, and Assay Information File, is available for download at thermofisher.com/taqmanfiles. The Assay Information File provides detailed information such as sales order details, list of contents (including assay ID, part number, expiration date, and lot number), annotation information (gene symbol, gene name, Entrez Gene ID, transcript accession, and location on transcript), and design details (context sequence and amplicon size).
TaqMan fusion gene expression assay resources
- Assay ID list:TaqMan Gene Expression Assays for fusion transcripts
Understanding predesigned single tube gene expression assays suffix nomenclature
Each TaqMan gene expression assay is labeled with specific suffixes that provide detailed information about the assay's design and application. These suffixes help researchers quickly identify the characteristics and intended use of each assay.
Non-coding RNA assays
- _s1: Indicates that the primers and probes are designed within a single exon. These assays will detect genomic DNA because the probe does not span an exon-exon junction.
- _g1: Similar to _s1, these assays may detect genomic DNA as the primers and probe are within a single exon. The design ensures that the assay can amplify both cDNA and genomic DNA.
- _m1: The probe spans an exon junction, which means it will not detect genomic DNA. This design is ideal for distinguishing between mRNA and genomic DNA, ensuring specificity for the target transcript.
Marker and reporter assays
- _mr: Assays that end in _mr indicate a TaqMan marker and reporter assay. These assays are designed to measure the expression of specific marker genes and reporter genes, which are often used in various experimental setups, including gene expression studies and functional assays.
Fusion gene assays
- _f1: This suffix is used for assays designed to detect fusion genes. Fusion genes result from chromosomal rearrangements and are important in cancer research and diagnostics. The probe and primers are designed to span the fusion junction, ensuring specific detection of the fusion transcript.
High homology assays
- _mH, _sH, _gH: These suffixes indicate that the assay was designed to a transcript belonging to a gene family with high sequence homology. The assays are optimized to provide between 1,000 to 30,000-fold greater discrimination from the closest homologous transcript, ensuring high specificity even in the presence of similar sequences.
Universal assays
- _u: Indicates an assay whose amplicon spans an exon junction, and the probe sits completely in one exon. This design ensures that the assay can detect multiple transcript variants of a gene, providing a more comprehensive analysis of gene expression.
By understanding these suffixes, researchers can select the most appropriate TaqMan gene expression assay for their specific experimental needs, helping ensure accurate and reliable results.
A convenient collection of TaqMan gene expression assays targeting common endogenous control candidates. Normalization to endogenous control genes is currently the most accurate method to correct for potential biases present in RNA or DNA samples.
Explore:TaqMan endogenous control assays
Learn how to easily order both FAM and VIC-labeled TaqMan Gene Expression Assays to enable simultaneous detection of both your endogenous control and gene-of-interest.
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For Research Use Only. Not for use in diagnostic procedures.
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