microdialysis plates

Pierce Microdialysis Plates offer a ready-to-use disposable dialysis solution designed for multiplexing of many small volume samples, up to 96 x 0.1–0.3 mL samples (or 48 x 0.3–1.0 mL samples) in parallel with a variety of MWCOs. The devices feature regenerated cellulose membranes with a very large surface-area-to-volume ratio for dialysis experiments that take just 2–4 hours to complete, and provide greater than 90% protein recovery.

Pierce Microdialysis Plates are commonly used for a variety of protein sample cleanup purposes including protein and DNA desalting/buffer exchange, small molecule contaminates and unused reagent removal, and removal of dyes after protein labeling.

Choose the right Pierce Microdialysis device for your experiments

 Image of 96-well, 100ul volume plate setupImage of 96-well, 300ul volume plate setupImage of 48-well, 1ml volume plate setup
Plate size96-well96-well48-well
Max volume

0.1 mL

0.3 mL

1 mL

See all Pierce Microdialysis plates

Recommended accessories

96-well deep-well plate, 2.2 mL—polypropylene deep-well assay plate is required for 96-well microdialysis devices; for 48-well microdialysis kits, the deep-well plate is provided by default.

Plate seal—adhesive-coated plastic sheets are recommended to seal 96-well or 48-well microdialysis plates, to protect against contamination and evaporation, and are also suitable for long-term storage.

Catalog # Name Size Price (USD) Qty
A50462 Pierce™ Microdialysis Plate, 96 well, 0.1 mL, 2K MWCO, 1 Plate Set Each
579.65

Online Exclusive

622.00
Save 42.35 (7%)
A50465 Pierce™ Microdialysis Plate, 96 well, 0.3 mL, 2K MWCO, 1 Plate Set Each
604.65

Online Exclusive

642.00
Save 37.35 (6%)
A50466 Pierce™ Microdialysis Plate, 48 well, 1 mL, 2K MWCO, 1 Plate Set Each
521.65

Online Exclusive

554.00
Save 32.35 (6%)
88262 Pierce™ 96-well Microdialysis Plate, 3.5K MWCO, 1 Plate Set Each
585.65

Online Exclusive

622.00
Save 36.35 (6%)
A50467 Pierce™ Microdialysis Plate, 96 well, 0.3 mL, 3.5K MWCO, 1 Plate Set Each
604.65

Online Exclusive

642.00
Save 37.35 (6%)
A50468 Pierce™ Microdialysis Plate, 48 well, 1 mL, 3.5K MWCO, 1 Plate Set Each
509.65

Online Exclusive

554.00
Save 44.35 (8%)
88260 Pierce™ 96-well Microdialysis Plate, 10K MWCO, 1 Plate Set Each
579.65

Online Exclusive

624.00
Save 44.35 (7%)
A50469 Pierce™ Microdialysis Plate, 96 well, 0.3 mL, 10K MWCO, 1 Plate Set Each
590.65

Online Exclusive

642.00
Save 51.35 (8%)
A50470 Pierce™ Microdialysis Plate, 48 well, 1 mL, 10K MWCO, 1 Plate Set Each
507.65

Online Exclusive

554.00
Save 46.35 (8%)
A50471 Pierce™ Microdialysis Plate, 96 well, 0.1 mL, 20K MWCO, 1 Plate Set Each
585.65

Online Exclusive

622.00
Save 36.35 (6%)
A50472 Pierce™ Microdialysis Plate, 96 well, 0.3 mL, 20K MWCO, 1 Plate Set Each
597.65

Online Exclusive

642.00
Save 44.35 (7%)
A50473 Pierce™ Microdialysis Plate, 48 well, 1 mL, 20K MWCO, 1 Plate Set Each
507.65

Online Exclusive

554.00
Save 46.35 (8%)
88261 96-well Deep-well Plate, 2.2mL, for Pierce™ 96-well Microdialysis, 1 Plate Each
32.65

Online Exclusive

34.27
Save 1.62 (5%)
88269 Plate Seal for Pierce™ 96-well Microdialysis Plates Each
23.32
Microdialysis reference figures

Each microdialysis plate includes 12 8-dialysis device strips which can be removed from the plate and separated into individual devices. This reduces waste allowing for only the number of dialysis devices needed. The 96-well microdialysis plate procedure involves simply removing the number of dialysis devices needed for your experiment, adding dialysis buffer to your deep well plate or microcentrifuge tube, loading your sample into the microdialysis device, placing the device with sample into the plate prepared with buffer, dialyzing, and recovering your sample with a pipet (Figure 1). Dialysis time will vary depending on salt and molecular weight concentrations.

Figure 1. Step-by-step procedure for the Pierce 96-well Microdialysis Plates

Microdialysis devices are offered at 2K, 3.5K, 10K, and 20K MWCOs. Experimental data demonstrate that the rate of dialysis, as shown in conductivity (milliSiemens (mS)), is similar for the various 100 μL microdialysis device MWCOs (Figure 2). When evaluating efficiency of timed dialysis changes, conductivity decreases significantly in just 30 minutes, demonstrating that the majority of low molecular weight compounds are removed from the samples (Figure 3).

Figure 2. Similar rate of dialysis across different MWCOs—100 µL. Samples of 0.1 mL (0.5 mg/mL Human IgG containing 1 M NaCl) were dialyzed against 1.8 mL of water in a 96 deep-well plate at room temperature. The water was changed at 30 minute intervals over a 2.5 hour period. The rate of NaCl removal was determined by measuring the conductivity of the sample at the indicated time intervals.

Figure 3. Comparing of efficiency of timed dialysis changes—96 well. Samples of 0.1 mL (0.5 mg/mL Human IgG containing 1 M NaCl) were dialyzed against 1.8 mL of water in a 96 deep-well plate at room temperature. The water was changed at 15-, 30-, and 1 hour intervals over a 3 hour period. The rate of NaCl removal was determined by measuring the conductivity of the sample at the indicated time intervals.

Image depicting a variety of volumes and MWCO’s of the Slide-A-Lyzer G3

Need to dialyze a larger volume sample?

Try Thermo Scientific Slide-A-Lyzer G3 Cassettes or Slide-A-Lyzer Dialysis Flasks for volumes from 1 mL to 250 mL. Slide-A-Lyzer dialysis cassettes and flasks offer a user-friendly approach to traditional dialysis tubing by incorporating dialysis membranes into a rigid frame with a secure cap which allows for easier sample loading/retrieval and increased sample security.

Find out more

For Research Use Only. Not for use in diagnostic procedures.