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Protein Concentrators
Protein extraction, purification, and cleanup methods frequently produce dilute samples of target biomolecules in buffer matrices incompatible with downstream applications. Pierce protein concentrators are disposable ultrafiltration centrifugal devices used to concentrate, desalt, and buffer exchange biological samples, such as proteins and nucleic acids, readying them for downstream applications with minimal sample handling. Pierce protein concentrators are available in four sizes (0.5, 6, 20, and 100 mL) and offer pH compatibility over a broad pH range (1-9).
For larger volume sizes, Pierce crossflow filtration cassettes are designed for high purity and recovery of peptides, proteins, and virus/viral particles. They are used to concentrate large supernatant volumes (0.5 L–6 L). Additional accessories are available for use with the crossflow filtration cassettes.
Choose the right protein concentrator or crossflow filtration device
| Protein concentration | NEW Crossflow filtration cassettes | ||||
|---|---|---|---|---|---|
 |  |  |  |  | |
| Sample volume | 0.1 mL–0.5 mL | 2 mL–6 mL | 5 mL–20 mL | 20 mL–100 mL | 0.5 L–6 L |
| See all Protein Concentrators, 3K or 5K MWCO * | See all Crossflow Filtration Cassettes, 3-100 kDa MWCO | ||||
| See all Protein Concentrators, 10K MWCO | |||||
| See all Protein Concentrators, 30K MWCO | |||||
| See all Protein Concentrators, 50K MWCO | |||||
| See all Protein Concentrators, 100K MWCO | |||||
*MWCO = molecular weight cutoff
Recommended accessories for Pierce Crossflow Filtration Cassettes
Ordering information
Product benefits
Protein concentrators
- Fast—concentrate samples up to 10- to 50-fold in 5–30 minutes (times may vary with different MWCOs)
- Flexible—suitable for sample volumes ranging from 100 μL to 100 mL, with a wide range of MWCO offerings
- Efficient—recovery of >90% of target sample while removing contaminants or exchange buffers
- Convenient—clear markings, wide sample chamber, and removable filtrate chamber make handling simple and easy
- Instrument compatible—can be used with standard centrifuges with either fixed-angle or swinging-bucket rotors
Crossflow filtration cassettes
- Scalable—concentrate sample volumes ranging from 0.5–6 L
- Fast—many concentration and protein recovery protocols can be completed in 30 or less minutes
- Versatile—concentration cassettes are available in five different MWCOs and are compatible with buffers up to pH 13
- Efficient—recovery of >99% protein (with 25 mL buffer rinse)
How-to-videos for each protein concentrator size
0.1–0.5 mL concentrators
Watch how to concentrate protein solution for sample volumes up to 0.5 mL using the microcentrifuge tube format. Membranes are available with 3K, 10K, 30K, 50K, and 100K MWCO.
2–6 mL concentrators
Watch how to concentrate protein solution for sample volumes up to 6 mL using the 15 mL centrifuge tube format. Membranes are available with 3K, 10K, 30K, 50K, or 100K MWCO.
5–20 mL concentrators
Watch how to concentrate protein solution for sample volumes up to 20 mL using the 20 mL tube format. Membranes are available with 3K, 10K, 30K, 50K, and 100K MWCO.
20–100 mL concentrators
Watch how to concentrate protein solution for sample volumes up to 100 mL using the 250 mL centrifuge bottle format. Membranes are available with 5K, 10K, 30K, 50K, and 100K MWCO.
0.5–6 L concentrators
Watch how to concentrate protein solution for sample volumes up to 6 liters using the Crossflow Filtration Cassette. These tangential flow filtration (TFF) cassettes are available in 3K, 10K, 30K, 50K, and 100K MWCO.
Resources
Learning resources
Support resources
For Research Use Only. Not for use in diagnostic procedures.