APC-eFluor 780 excitation shown as dotted line and emission shown as solid red histogram
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flow cytometry

Invitrogen APC-eFluor 780 dye is a near-infrared fluorescent dye designed to be a high-performance tandem fluorophore for multicolor flow cytometry. It is designed for use with flow cytometers equipped with a red laser and have an emission peak of 780 nm. With similar spectral characteristics to APC-Alexa Fluor 750, APC-eFluor 780 requires no changes to your instrument configuration for use, allowing you to easily add APC-eFluor 780 to your multicolor panels.

We offer APC-eFluor 780 dye conjugated to primary antibodies for use in flow cytometry.

Search APC-Fluor 780 primary antibodies

  • Equal or better sensitivity than APC-H7 tandem conjugates
  • Stable performance when treated with formaldehyde fixation or exposed to ambient light
  • Uses same filter sets as existing comparative fluorochromes
Flow cytometry histogram comparing APC-eFluor 780 (blue) to APC-H7 (red)

Comparison of APC-eFluor 780 dye to APC-H7 dye using flow cytometry. Mouse splenocytes were stained with CD8a Monoclonal Antibody (53-6.7), APC-eFluor 780 (blue line) or CD8a monoclonal antibody conjugated to APC-H7 (red line).

Flow cytometry histogram of PBMCs comparing APC-eFluor 780 before (red, blue) and after aldehyde fixation (green)
Stability analysis of APC-eFluor 780. Human peripheral blood mononuclear cells (PBMCs) were stained with CD8a Monoclonal Antibody (RPA-T8), APC-eFluor 780 and analyzed immediately (red line) on flow cytometer. The sample was then divided and either exposed to ambient light for six hours (blue line) or fixed in 2% formaldehyde for 30 minutes at room temperature (green line) and analyzed again. 

Additional resources

Flow cytometry protocols handbook

Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.

Request the handbook

 

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