Qdot 705 spectra excitation shown as dotted line and emission shown as solid red histogram
5350 or 633695/40300702(in buffer) 5microscopy, flow cytometry

Invitrogen Qdot 705 probe is an excellent near-IR–fluorescent label with outstanding brightness and photostability for imaging and flow cytometry applications. With an emission maximum of ~705 nm, Qdot 705 probe can be excited using a laser line from 350 nm up to 633 nm or a standard Cy5.5 filter. Optimum imaging results with brighter signals are achieved with a Qdot 705 filter or light cube.

Organic fluorophores and Qdot probes can be readily combined in multiplexed flow cytometry or imaging studies. Longer-wavelength Qdot probes are also well-suited for multiplexed western blots.

We offer Qdot 705 probe conjugated to a variety of antibodies, peptides, and proteins, optimized for cellular labeling and detection. In addition, reactive dye forms and antibody labeling kits are available to allow you to generate your own antibody conjugates or probes.

Qdot Label Conjugates for Cell and Tissue Staining    Search Qdot 705 primary antibodies

Experimental data using Qdot 705 probe
Flow histogram overlay plot of unstained (gray line) vs. Qdot 705-stained HLA-DR (black line)
Flow cytometry analysis of human lymphocytes using Qdot 705 antibody conjugate. Histogram overlay plot of gated human lymphocytes, the black line represents cells labeled with mouse anti-human HLA-DR Monoclonal Antibody (TU36), Qdot 705 and the gray line represents unstained cells. Samples were acquired and analyzed using 405 nm excitation and a 695/40 band pass emission filter using the Attune Acoustic Cytometer Blue/Violet option.

Qdot Probes

Qdot probes are made from nanocrystals of semiconductor material and are broadly compatible with standard fluorescence techniques, but there are some novel aspects to their use that can be optimized to obtain the best possible results. The exceptional brightness and photostability of Qdot probes make it easier to detect low-abundance targets. With narrow and symmetric emission spectra, Qdot probes are also well-suited for multiplexed fluorescence detection using a combination of Qdot probes and organic dyes.

Additional resources

Flow cytometry protocols handbook

Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.

Request the handbook

 

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Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.

For Research Use Only. Not for use in diagnostic procedures.