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FastDigest Restriction Enzyme Selection Tool |
The FastDigest Restriction Enzyme Selection Tool helps users quickly find the best FastDigest restriction enzymes by organizing them based on recognition sequences, optimal conditions, and DNA compatibility. It streamlines experimental planning, enhances workflow efficiency, and supports successful outcomes.
Search FastDigest restriction enzymes by name, isoschizomers name, sequence, optimal temperature, or methylation effects
Use the tool below to find restriction enzymes by name, recognition sequence, thermal inactivation temperature, or optimal temperature.
Isoschizomers
An isoschizomer restriction enzyme is a type of enzyme that recognizes and cleaves the same DNA sequence as another restriction enzyme but may originate from a different species of bacteria. Despite recognizing the same sequence, isoschizomers can sometimes have different reaction conditions or methylation sensitivities. This characteristic makes isoschizomers useful in various molecular biology applications, such as analyzing DNA methylation patterns or optimizing cloning strategies.
Methylation sensitivity
Methylation can affect a restriction enzyme’s activity in three different ways:
- Blocking cleavage—Some restriction enzymes are sensitive to methylation and will not cut DNA if their recognition site is methylated because the methyl group can physically block the enzyme from binding to the DNA or alter the DNA structure in a way that prevents recognition.
- Methylation dependence—Some restriction enzymes specifically recognize and cleave methylated DNA sequences; these enzymes are used in epigenetic studies to analyze DNA methylation patterns.
- No effect—Other restriction enzymes are insensitive to methylation and will cut DNA regardless of whether their recognition site is methylated or not. Understanding the methylation sensitivity of restriction enzymes is crucial for designing experiments, especially when working with genomic DNA or studying epigenetic modifications.
| Catalog # | Product | Isoschizomers | Cut site and sequence | Overhang | Optimal reaction temperature | Thermal inactivation | Methylation effects |
|---|---|---|---|---|---|---|---|
| FD0293 | FastDigest Eco31I (IIS class) | BsaI, Bso31I, BspTNI | GGTCTCN^NNNN CCAGAGNNNNN^ GGTCTCNNNNN | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect. |
| FD0294 | FastDigest Eco31I (IIS class) | BsaI, Bso31I, BspTNI | GGTCTCN^NNNN CCAGAGNNNNN^ GGTCTCNNNNN | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect. |
| FD0454 | FastDigest Esp3I (IIS class) | BsmBI, BstGZ53I, Esp16I, Esp23I | CGTCTCN^NNNN GCAGAGNNNNN^ CGTCTCNNNNN | 5’ protruding ends | 37° C | 65°C, 10 min | Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. |
| FD1014 | FastDigest BpiI (IIS class) | BbsI, BpuAI, BstV2I | GAAGACNN^NNNN CTTCTGNNNNNN^ GAAGACNNNNNN | 5’ protruding ends | 37° C | 65°C, 10 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1074 | FastDigest MnlI (IIS class) | CCTCNNNNNNN^ GGAGNNNNNN^N CCTCNNNNNNN | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped. | |
| FD0034 | FastDigest Alw26I (IIS class) | BsmAI, BstMAI | GTCTCN^NNNN CAGAGNNNNN^ GTCTCNNNNN | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD0824 | FastDigest MboII (IIS class) | GAAGANNNNNNNN^ CTTCTNNNNNNN^N GAAGANNNNNNNN | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: blocked if overlapped. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD2124 | FastDigest BmsI (IIS class) | BhaI, BscAI, BscUI, BsmNI, BspST5I, Bst19I, CjeP338II, LweI, Mva1312II, PhaI, SfaNI | GCATCNNNNN^NNNN CGTAGNNNNNNNNN^ GCATCNNNNNNNNN | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. |
| FD0964 | FastDigest Mva1269I (IIS class) | BsaMI, BsmI, PctI | GAATGCN^ CTTAC^GN GAATGCN | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1934 | FastDigest LguI (IIS class) | BspQI, PciSI, SapI | GCTCTTCN^NNN CGAGAAGNNNN^ CTCTTCNNNN | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0874 | FastDigest BseGI (IIS class) | BstF5I, BtsCI, FokI | GGATGNN^ CCTAC^NN GGATGNN | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0014 | FastDigest AluI | AluBI | AG^CT TC^GA AGCT | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped. |
| FD0024 | FastDigest Alw21I | Bbv12I, BsiHKAI | GWGCW^C C^WCGWG GWGCWC | 3’ protruding ends | 37° C | 80°C, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0044 | FastDigest Alw44I | ApaLI, VneI | G^TGCAC CACGT^G GTGCAC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD0054 | FastDigest BamHI | G^GATCC CCTAG^G GGATCC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD0055 | FastDigest BamHI | G^GATCC CCTAG^G GGATCC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD0064 | FastDigest BcnI | AsuC2I, BpuMI, NciI | CC^SGG GGS^CC CCSGG | 5’ protruding ends | 37° C | 80°C, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
| FD0074 | FastDigest BglI | GCCNNNN^NGGC CGGN^NNNNCCG GCCNNNNNGGC | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. | |
| FD0083 | FastDigest BglII | A^GATCT TCTAG^A AGATCT | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: impaired if overlapped. | |
| FD0084 | FastDigest BglII | A^GATCT TCTAG^A AGATCT | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: impaired if overlapped. | |
| FD0094 | FastDigest Bpu1102I | BlpI, Bsp1720I, CelII | GC^TNAGC CGANT^CG GCTNAGC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0124 | FastDigest Bsp119I | AsuII, Bpu14I, BspT104I, BstBI, Csp45I, NspV, SfuI | TT^CGAA AAGC^TT TTCGAA | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoBI: no effect. |
| FD0134 | FastDigest Bsp120I | PspOMI | G^GGCCC CCCGG^G GGGCCC | 5’ protruding ends | 37° C | 80°C, 10 min | Dam: no effect. Dcm: blocked if overlapped. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD0143 | FastDigest Bsu15I | BanIII, Bsa29I, BseCI, BshVI, BspDI, BspXI, BsuTUI, ClaI | AT^CGAT TAGC^TA ATCGAT | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: blocked if overlapped. Dcm: no effect. CpG: blocked. EcoKI: no effect. |
| FD0144 | FastDigest Bsu15I | BanIII, Bsa29I, BseCI, BshVI, BspDI, BspXI, BsuTUI, ClaI | AT^CGAT TAGC^TA ATCGAT | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: blocked if overlapped. Dcm: no effect. CpG: blocked. EcoKI: no effect. |
| FD0154 | FastDigest BsuRI | BshFI, BsnI, BspANI, HaeIII, PhoI | GG^CC CC^GG GGCC | blunt | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0184 | FastDigest Cfr10I | Bse118I, BsrFI, BssAI | R^CCGGY YGGCC^R RCCGGY | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0194 | FastDigest Cfr13I | AspS9I, BmgT120I, PspPI, Sau96I | G^GNCC CCNG^G GGNCC | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: blocked if overlapped. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD0214 | FastDigest Csp6I | AfaI, CviQI, RsaNI | G^TAC CAT^G GTAC | 5’ protruding ends | 37° C | 80°C, 10 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0224 | FastDigest DraI | TTT^AAA AAA^TTT TTTAAA | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: blocked if overlapped. EcoBI: no effect. | |
| FD0234 | FastDigest Eam1104I | Bst6I, EarI | CTCTTCN^NNN GAGAAGNNNN^ CTCTTCNNNN | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0244 | FastDigest Eam1105I | AhdI, AspEI, BmeRI, DriI, EclHKI | GACNNN^NNGTC CTGNN^NNNCAG GACNNNNNGTC | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0254 | FastDigest Ecl136II | Eco53kI, EcoICRI, Psp124BI, SstI | GAG^CTC CTC^GAG GAGCTC | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. |
| FD0264 | FastDigest EcoO109I | DraII | RG^GNCCY YCCNG^GR RGGNCCY | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect. |
| FD0274 | FastDigest EcoRI | G^AATTC CTTAA^G GAATTC | 5’ protruding ends | 37° C | 80°C, 5min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. | |
| FD0275 | FastDigest EcoRI | G^AATTC CTTAA^G GAATTC | 5’ protruding ends | 37° C | 80°C, 5min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. | |
| FD0303 | FastDigest Eco32I | EcoRV | GAT^ATC CTA^TAG GATATC | blunt | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0304 | FastDigest Eco32I | EcoRV | GAT^ATC CTA^TAG GATATC | blunt | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0314 | FastDigest Eco47I | AvaII, Bme18I, SinI, VpaK11BI | G^GWCC CCWG^G GGWCC | 5’ protruding ends | 37° C | 80°C, 20 min | Dam: no effect. Dcm: blocked if overlapped. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD0324 | FastDigest Eco47III | AfeI, Aor51HI | AGC^GCT TCG^CGA AGCGCT | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. |
| FD0334 | FastDigest Eco52I | BseX3I, BstZI, EagI, EclXI | C^GGCCG GCCGG^C CGGCCG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0344 | FastDigest Eco57I | AcuI, Bsp6II, BspD6II, BspKT5I, Eco112I, Eco125I, FsfI, SstE37III | CTGAAGNNNNNNNNNNNNNNNN^ GACTTCNNNNNNNNNNNNNN^NN CTGAAGNNNNNNNNNNNNNNNN | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0364 | FastDigest Eco72I | AcvI, BbrPI, PmaCI, PmlI, PspCI | CAC^GTG GTG^CAC CACGTG | blunt | 37° C | 80°C, 10 min | Dam: no effect. Dcm: no effect. CpG: blocked. |
| FD0374 | FastDigest Eco81I | AxyI, Bse21I, Bsu36I | CC^TNAGG GGANT^CC CCTNAGG | 5’ protruding ends | 37° C | 80°C, 10 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0384 | FastDigest Eco88I | Ama87I, AvaI, BmeT110I, BsiHKCI, BsoBIm | C^YCGRG GRGCY^C CYCGRG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
| FD0394 | FastDigest Eco91I | BstEII, BstPI, EcoO65I, PspEI | G^GTNACC CCANTG^G GGTNACC | 5’ protruding ends | 37° C | 65°C, 10 min | Dam: no effect. Dcm: no effect. CpG: no effect. |
| FD0404 | FastDigest Eco105I | BstSNI, SnaBI | TAC^GTA ATG^CAT TACGTA | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0414 | FastDigest Eco130I | BssT1I, EcoT14I, ErhI, StyI | C^CWWGG GGWWC^C CCWWGG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0424 | FastDigest Eco147I | AatI, PceI, SseBI, StuI | AGG^CCT TCC^GGA AGGCCT | blunt | 37° C | 80°C, 10 min | Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect. |
| FD0434 | FastDigest ScaI | AssI, BmcAI, ZrmI | AGT^ACT TCA^TGA AGTACT | blunt | 37° C | 65°C, 10 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped. |
| FD0443 | FastDigest EheI | BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI | GGC^GCC CCG^CGG GGCGCC | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0444 | FastDigest EheI | BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI | GGC^GCC CCG^CGG GGCGCC | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0464 | FastDigest GsuI | Bco35I, BpmI, Bsp22I, Bsp28I, BspJ74I, Bth1795I, Uba1437I, Uba1444I | CTGGAGNNNNNNNNNNNNNNNN^ GACCTCNNNNNNNNNNNNNN^NN CTGGAGNNNNNNNNNNNNNNNN | 3’ protruding ends | 30° C | 65°C, 5 min | Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0474 | FastDigest Hin1I | AcyI, BsaHI, BssNI, BstACI, Hsp92I | GR^CGYC CYGC^RG GRCGYC | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: impaired if overlapped. CpG: blocked. EcoKI: no effect. |
| FD0484 | FastDigest Hin6I | AspLEI, BstHHI, CfoI, HinP1I, HspAI | G^CGC CGC^G GCGC | 5’ protruding ends | 37° C | 80°C, 10 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0494 | FastDigest HincII | HindII | GTY^RAC CAR^YTG GTYRAC | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: blocked if overlapped. EcoBI: blocked if overlapped. |
| FD0504 | FastDigest HindIII | A^AGCTT TTCGA^A AAGCTT | 5’ protruding ends | 37° C | 80°C, 10 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: impaired if overlapped. | |
| FD0505 | FastDigest HindIII | A^AGCTT TTCGA^A AAGCTT | 5’ protruding ends | 37° C | 80°C, 10 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. | |
| FD0514 | FastDigest HpaII | BsiSI, HapII | C^CGG GGC^C CCGG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0524 | FastDigest KpnI | Asp718I | GGTAC^C C^CATGG GGTACC | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0534 | FastDigest Kpn2I | AccIII, Aor13HI, BseAI, Bsp13I, BspEI, MroI | T^CCGGA AGGCC^T TCCGGA | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0544 | FastDigest MspI | BsiSI, HapII | C^CGG GGC^C CCGG | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0554 | FastDigest MvaI | AjnI, BseBI, Bst2UI, BstNI, BstOI, Psp6I, PspGI | CC^WGG GGW^CC CCWGG | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0564 | FastDigest MluI | A^CGCGT TGCGC^A ACGCGT | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. | |
| FD0573 | FastDigest NcoI | Bsp19I | C^CATGG GGTAC^C CCATGG | 5’ protruding ends | 37° C | 65°C, 15 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0574 | FastDigest NcoI | Bsp19I | C^CATGG GGTAC^C CCATGG | 5’ protruding ends | 37° C | 65°C, 15 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0575 | FastDigest NcoI | Bsp19I | C^CATGG GGTAC^C CCATGG | 5’ protruding ends | 37° C | 65°C, 15 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0583 | FastDigest NdeI | FauNDI | CA^TATG GTAT^AC CATATG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0584 | FastDigest NdeI | FauNDI | CA^TATG GTAT^AC CATATG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0585 | FastDigest NdeI | FauNDI | CA^TATG GTAT^AC CATATG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0593 | FastDigest NotI | CciNI | GC^GGCCGC CGCCGG^CG GCGGCCGC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0594 | FastDigest NotI | CciNI | GC^GGCCGC CGCCGG^CG GCGGCCGC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0595 | FastDigest NotI | CciNI | GC^GGCCGC CGCCGG^CG GCGGCCGC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0596 | FastDigest NotI | CciNI | GC^GGCCGC CGCCGG^CG GCGGCCGC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0604 | FastDigest PaeI | BbuI, SphI | GCATG^C C^GTACG GCATGC | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped. |
| FD0614 | FastDigest PstI | BspMAI | CTGCA^G G^ACGTC CTGCAG | 3’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0615 | FastDigest PstI | BspMAI | CTGCA^G G^ACGTC CTGCAG | 3’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0624 | FastDigest PvuI | BpvUI, MvrI, Ple19I | CGAT^CG GC^TAGC CGATCG | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0634 | FastDigest PvuII | CAG^CTG GTC^GAC CAGCTG | blunt | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. | |
| FD0644 | FastDigest SalI | G^TCGAC CAGCT^G GTCGAC | 5’ protruding ends | 37° C | 65°C, 10 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. | |
| FD0654 | FastDigest SduI | Bsp1286I, MhlI | GDGCH^C C^HCGDG GDGCHC | 3’ protruding ends | 37° C | 80°C, 15 min | Dam: no effect. Dcm: no effect. CpG: no effect. |
| FD0663 | FastDigest SmaI | TspMI, XmaCI, XmaI | CCC^GGG GGG^CCC CCCGGG | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0664 | FastDigest SmaI | TspMI, XmaCI, XmaI | CCC^GGG GGG^CCC CCCGGG | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0674 | FastDigest TaqI | T^CGA AGC^T TCGA | 5’ protruding ends | 65° C | No (column purification) | Dam: blocked if overlapped. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD0684 | FastDigest XbaI | T^CTAGA AGATC^T TCTAGA | 5’ protruding ends | 37° C | 65°C, 20 min | Dam: blocked if overlapped. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD0685 | FastDigest XbaI | T^CTAGA AGATC^T TCTAGA | 5’ protruding ends | 37° C | 65°C, 20 min | Dam: blocked if overlapped. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD0694 | FastDigest XhoI | PaeR7I, Sfr274I, SlaI, StrI, TliI | C^TCGAG GAGCT^C CTCGAG | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
| FD0695 | FastDigest XhoI | PaeR7I, Sfr274I, SlaI, StrI, TliI | C^TCGAG GAGCT^C CTCGAG | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
| FD0704 | FastDigest Bst1107I | BssNAI, BstZ17I | GTA^TAC CAT^ATG GTATAC | blunt | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD0714 | FastDigest Van91I | AccB7I, BasI, PflMI | CCANNNN^NTGG GGTN^NNNNACC CCANNNNNTGG | 3’ protruding ends | 37° C | 65°C, 10 min | Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0724 | FastDigest BclI | FbaI, Ksp22I | T^GATCA ACTAG^T TGATCA | 5’ protruding ends | 37° C | No (column purification) | Dam: blocked. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped. |
| FD0734 | FastDigest Mph1103I | EcoT22I, NsiI, Zsp2I | ATGCA^T T^ACGTA ATGCAT | 3’ protruding ends | 37° C | 65°C, 15 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0744 | FastDigest CpoI | CspI, Rsr2I, RsrII | CG^GWCCG GCCWG^GC CGGWCCG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0753 | FastDigest MunI | MfeI | C^AATTG GTTAA^C CAATTG | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0754 | FastDigest MunI | MfeI | C^AATTG GTTAA^C CAATTG | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0764 | FastDigest Psp5II | PpuMI, PspPPI | RG^GWCCY YCCWG^GR RGGWCCY | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect. |
| FD0774 | FastDigest SspI | AAT^ATT TTA^TAA AATATT | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD0784 | FastDigest Bsp143I | BfuCI, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIm | ^GATC CTAG^ GATC | 5’ protruding ends | 37° C | 65°C, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD0804 | FastDigest HinfI | G^ANTC CTNA^G GANTC | 5’ protruding ends | 37° C | 65°C, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: blocked if overlapped. | |
| FD0814 | FastDigest MboI | BfuCI, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIm | ^GATC CTAG^ GATC | 5’ protruding ends | 37° C | 65°C, 15 min | Dam: blocked. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped. |
| FD0834 | FastDigest BspTI | AflII, BfrI, Bst98I, BstAFI, MspCI, Vha464I | C^TTAAG GAATT^C CTTAAG | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0854 | FastDigest Pfl23II | BsiWI, PspLI | C^GTACG GCATG^C CGTACG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0884 | FastDigest BseNI | Bse1I, BsrI, BsrSI | ACTGGN^ TGAC^CN ACTGGN | 3’ protruding ends | 65° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. |
| FD0894 | FastDigest Bsh1285I | BsiEI, BstMCI | CGRY^CG GC^YRGC CGRYCG | 3’ protruding ends | 37° C | 80°C, 15 min | Dam: impaired if overlapped. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0904 | FastDigest Acc65I | Asp718I | G^GTACC CCATG^G GGTACC | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD0914 | FastDigest VspI | AseI, PshBI | AT^TAAT TAAT^TA ATTAAT | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD0924 | FastDigest Bsh1236I | AccII, BspFNI, BstFNI, BstUI, MvnI | CG^CG GC^GC CGCG | blunt | 37° C | 80°C, 10 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD0933 | FastDigest Bsp1407I | BsrGI, BstAUI | T^GTACA ACATG^T TGTACA | 5’ protruding ends | 37° C | 80°C, 10 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0934 | FastDigest Bsp1407I | BsrGI, BstAUI | T^GTACA ACATG^T TGTACA | 5’ protruding ends | 37° C | 80°C, 10 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD0944 | FastDigest Psp1406I | AclI | AA^CGTT TTGC^AA AACGTT | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. |
| FD0973 | FastDigest NheI | AsuNHI, BmtI | G^CTAGC CGATC^G GCTAGC | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD0974 | FastDigest NheI | AsuNHI, BmtI | G^CTAGC CGATC^G GCTAGC | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD0984 | FastDigest Tru1I | MseI, Tru9I | T^TAA AAT^T TTAA | 5’ protruding ends | 65° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: blocked if overlapped. EcoBI: no effect. |
| FD0994 | FastDigest AatII | ZraI | GACGT^C C^TGCAG GACGTC | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD1004 | FastDigest BshNI | AccB1I, BanI, BspT107I | G^GYRCC CCRYG^G GGYRCC | 5’ protruding ends | 37° C | 65°C, 10 min | Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoBI: no effect. |
| FD1024 | FastDigest BstXI | CCANNNNN^NTGG GGTN^NNNNNACC CCANNNNNNTGG | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: impaired if overlapped. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD1034 | FastDigest KspAI | HpaI | GTT^AAC CAA^TTG GTTAAC | blunt | 37° C | 65°C, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: blocked if overlapped. EcoBI: no effect. |
| FD1084 | FastDigest BseDI | BsaJI, BssECI | C^CNNGG GGNNC^C CCNNGG | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD1124 | FastDigest RsaI | AfaI, CviQI, RsaNI | GT^AC CA^TG GTAC | blunt | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1133 | FastDigest SacI | Eco53kI, EcoICRI, Psp124BI, SstI | GAGCT^C C^TCGAG GAGCTC | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD1134 | FastDigest SacI | Eco53kI, EcoICRI, Psp124BI, SstI | GAGCT^C C^TCGAG GAGCTC | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD1144 | FastDigest TaiI | HpyCH4IV, MaeII | ACGT^ ^TGCA ACGT | 3’ protruding ends | 65° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: blocked. |
| FD1154 | FastDigest BspLI | BmiI, NlaIV, PspN4I | GGN^NCC CCN^NGG GGNNCC | blunt | 37° C | 65°C, 20 min | Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1164 | FastDigest BfmI | BstSFI, SfcI | C^TRYAG GAYRT^C CTRYAG | 5’ protruding ends | 37° C | 65°C, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD1184 | FastDigest Bpu10I | CC^TNAGC GGANT^CG CCTNAGC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD1194 | FastDigest SdaI | SbfI, Sse8387I | CCTGCA^GG GG^ACGTCC CCTGCAGG | 3’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD1204 | FastDigest BseLI | AfiI, Bsc4I, BslI | CCNNNNN^NNGG GGNN^NNNNNCC CCNNNNNNNGG | 3’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1214 | FastDigest MlsI | BalI, MluNI, MscI, Msp20I | TGG^CCA ACC^GGT TGGCCA | blunt | 37° C | 80°C, 20 min | Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD1224 | FastDigest NsbI | Acc16I, AviII, FspI | TGC^GCA ACG^CGT TGCGCA | blunt | 37° C | 65°C, 15 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD1234 | FastDigest AdeI | DraIII | CACNNN^GTG GTG^NNNCAC CACNNNGTG | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. |
| FD1244 | FastDigest SmiI | SwaI | ATTT^AAAT TAAA^TTTA ATTTAAAT | blunt | 37° C | 65°C, 15 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1253 | FastDigest BcuI | AhlI, SpeI | A^CTAGT TGATC^A ACTAGT | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. |
| FD1254 | FastDigest BcuI | AhlI, SpeI | A^CTAGT TGATC^A ACTAGT | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. |
| FD1264 | FastDigest BseMI | Bse3DI, BsrDI | GCAATGNN^ CGTTAC^NN GCAATGNN | 3’ protruding ends | 55° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1274 | FastDigest MbiI | AccBSI, BsrBI | CCG^CTC GGC^GAG CCGCTC | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. |
| FD1284 | FastDigest PagI | CciI, BspHI, RcaI | T^CATGA AGTAC^T TCATGA | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: impaired if overlapped. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: impaired if overlapped. |
| FD1294 | FastDigest TatI | W^GTACW WCATG^W WGTACW | 5’ protruding ends | 65° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. | |
| FD1304 | FastDigest XagI | BstENI, EcoNI | CCTNN^NNNAGG GGANNN^NNTCC CCTNNNNNAGG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1314 | FastDigest BplI | GAAGACNN^NNNN CTTCTGNNNNNN^ GAAGACNNNNNN | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD1334 | FastDigest PsyI | AspI, PflFI, Tth111I | GACN^NNGTC CTGNN^NCAG GACNNNGTC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1344 | FastDigest MssI | PmeI | GTTT^AAAC CAAA^TTTG GTTT^AAAC | blunt | 37° C | 65°C, 10 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: blocked if overlapped. EcoBI: no effect. |
| FD1354 | FastDigest TasI | Sse9I, Tsp509I, TspEI | ^AATT TTAA^> AATT | 5’ protruding ends | 65° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked if overlapped. |
| FD1364 | FastDigest TaaI | Bst4CI, HpyCH4III | ACN^GT TG^NCA ACNGT | 3’ protruding ends | 65° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. |
| FD1374 | FastDigest SchI | MlyI, PleI, PpsI, SchI | GAGTCNNNNN^ CTCAGNNNNN GAGTCNNNNN | blunt | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1383 | FastDigest XapI | AcsI, ApoI | R^AATTY YTTAA^R RAATTY | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1384 | FastDigest XapI | AcsI, ApoI | R^AATTY YTTAA^R RAATTY | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1394 | FastDigest CaiI | AlwNI | CAGNNN^CTG GTC^NNNGAC CAGNNNCTG | 3’ protruding ends | 37° C | 65°C, 10 min | Dam: no effect. Dcm: blocked if overlapped. CpG: no effect. EcoKI: no effect. |
| FD1404 | FastDigest BseMII | BspCNI | GCAATGNN^ CGTTAC^NN GCAATGNN | 3’ protruding ends | 55° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD1414 | FastDigest ApaI | PspOMI | GGGCC^C C^CCGGG GGGCCC | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1424 | FastDigest Bme1390I | BmrFI, BssKI, BstSCI, MspR9I, ScrFI, StyD4I | CC^NGG GGN^CC CCNGG | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: blocked if overlapped. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1434 | FastDigest BoxI | BstPAI, PshAI | GACNN^NNGTC CTGNN^NNCAG GACNNNNGTC | blunt | 37° C | 80°C, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. |
| FD1444 | FastDigest BseSI | BaeGI, BstSLI | GKGCM^C C^MCGKG GKGCMC | 3’ protruding ends | 37° C | 80°C, 15 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: impaired if overlapped. EcoBI: no effect. |
| FD1454 | FastDigest BseXI | BbvI, BstV1I | GCAGCNNNNNNNN^NNNN CGTCGNNNNNNNNNNNN^ GCAGCNNNNNNNNNNNN | 5’ protruding ends | 65° C | 80°C, 15 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1464 | FastDigest BshTI | AgeI, AsiGI, CspAI, PinAI | A^CCGGT TGGCC^A ACCGGT | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. |
| FD1474 | FastDigest XceI | BstNSI, NspI | RCATG^Y Y^GTACR RCATGY | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD1484 | FastDigest XmiI | AccI, FblI | GT^MKAC CAKM^TG GTMKAC | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1514 | FastDigest NmuCI | Tsp45I | ^GTSAC CASTG^ GTSAC | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. |
| FD1524 | FastDigest PdiI | MroNI, NaeI, NgoMIV | GCC^GGC CGG^CCG GCCGGC | blunt | 37° C | 65°C, 15 min | Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1534 | FastDigest PdmI | Asp700I, MroXI, XmnI | GAANN^NNTTC CTTNN^NNAAG GAANNNNTTC | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. |
| FD1554 | FastDigest PsuI | BstX2I, BstYI, MflI, XhoII | R^GATCY YCTAG^R RGATCY | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1564 | FastDigest XmaJI | AspA2I, AvrII, BlnI | C^CTAGG GGATC^C CCTAGG | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD1574 | FastDigest Hpy8I | Hpy166II | GTN^NAC CAN^NTG GTNNAC | blunt | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. |
| FD1634 | FastDigest OliI | AleI | CACNN^NNGTG GTGNN^NNCAC CACNNNNGTG | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: blocked if overlapped. EcoBI: blocked if overlapped. |
| FD1644 | FastDigest SatI | Fnu4HI, Fsp4HI, ItaI | GC^NGC CGN^CG GCNGC | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1654 | FastDigest TauI | GCSG^C C^GSCG GCSGC | 3’ protruding ends | 55° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. | |
| FD1664 | FastDigest FspAI | RTGC^GCAY YACG^CGTR RTGCGCAY | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. | |
| FD1703 | FastDigest DpnI | MalI | GA^TC CT^AG GATC | blunt | 37° C | 80°C, 5 min | Dam: does not cut dam- DNA. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1704 | FastDigest DpnI | MalI | GA^TC CT^AG GATC | blunt | 37° C | 80°C, 5 min | Dam: does not cut dam - DNA. Dcm: no effect. Cpg: no effect. EcoKI: no effect. |
| FD1714 | FastDigest BseJI | BsaBI, Bse8I | GATNN^NNATC CTANN^NNTAG GATNNNNATC | blunt | 37° C | No (column purification) | Dam: blocked if overlapped. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1724 | FastDigest AasI | DrdI, DseDI | GACNNNN^NNGTC CTGNN^NNNNCAG GACNNNNNNGTC | 3’ protruding ends | 37° C | 80°C, 10 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1734 | FastDigest HpyF10VI | BstMWI, MwoI | GCNNNNN^NNGC CGNN^NNNNNCG GCNNNNNNNGC | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1744 | FastDigest BveI | Acc36I, BfuAI, BspMI | ACCTGCNNNN^NNNN TGGACGNNNNNNNN^ ACCTGCNNNNNNNN | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. |
| FD1754 | FastDigest PfoI | T^CCNGGA AGGNCC^T TCCNGGA | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: impaired if overlapped. Dcm: blocked if overlapped. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. | |
| FD1764 | FastDigest FspBI | BfaI, MaeI, XspI | C^TAG GAT^C CTAG | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD1784 | FastDigest PfeI | TfiI | G^AWTC CTWA^G GAWTC | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. |
| FD1794 | FastDigest SsiI | AciI, BspACI | C^CGC GGC^G CCGC | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD1814 | FastDigest FaqI | BslFI, BsmFI | GGGACNNNNNNNNNN^NNNN CCCTGNNNNNNNNNNNNNN^ GGGACNNNNNNNNNNNNNN | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD1824 | FastDigest SfiI | GGCCNNNN^NGGCC CCGGN^NNNNCCGG GGCCNNNNNGGCC | 3’ protruding ends | 50° C | No (column purification) | Dam: no effect. Dcm: impaired if overlapped. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. | |
| FD1834 | FastDigest Hin1II | CviAII, FaeI, FatI, Hsp92II, NlaIII | CATG^ ^GTAC CATG | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1854 | FastDigest HhaI | AspLEI, BstHHI, CfoI, HinP1I, HspAI | GCG^C C^GCG GCGC | 3’ protruding ends | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD1884 | FastDigest HpyF3I | BstDEI, DdeI | C^TNAG GANT^C CTNAG | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD1894 | FastDigest SgsI | AscI, PalAI | GG^CGCGCC CCGCGC^GG GGCGCGCC | 5’ protruding ends | 37° C | 65°C, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD1904 | FastDigest CseI | HgaI | GACGCNNNNN^NNNNN CTGCGNNNNNNNNNN^ GACGCNNNNNNNNNN | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. |
| FD1954 | FastDigest AjuI | NNNNN^NNNNNNNGAANNNNNNNTTGGNNNNNNNNNNN^ ^NNNNNNNNNNNNCTTNNNNNNNAACCNNNNNN^NNNNN NNNNNNNNNNNNGAANNNNNNNTTGGNNNNNNNNNNN | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. | |
| FD1974 | FastDigest Ppu21I | BsaAI, BstBAI | YAC^GTR RTG^CAY YACGTR | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked if overlapped. |
| FD2004 | FastDigest RseI | MslI, SmiMI | CAYNN^NNRTG GTRNN^NNYAC CAYNNNNRTG | blunt | 37° C | 65°C, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: blocked if overlapped. |
| FD2024 | FastDigest MreI | CG^CCGGCG GCGGCC^GC CGCCGGCG | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. | |
| FD2044 | FastDigest BspOI | AsuNHI, BmtI | GCTAG^C C^GATCG GCTAGC | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
| FD2064 | FastDigest AanI | PsiI | TTA^TAA AAT^ATT TTATAA | blunt | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoBI: no effect. |
| FD2074 | FastDigest Lsp1109I | BbvI, BstV1I | GCAGCNNNNNNNN^NNNN CGTCGNNNNNNNNNNNN^ GCAGCNNNNNNNNNNNN | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD2084 | FastDigest MauBI | CG^CGCGCG GCGCGC^GC CGCGCGCG | 3’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. | |
| FD2094 | FastDigest SfaAI | AsiSI, RgaI, SgfI | GCGAT^CGC CGC^TAGCG GCGATCGC | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD2104 | FastDigest TscAI | TspRI | NNCASTGNN^ ^NNGTSACNN NNCASTGNN | 3’ protruding ends | 65° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. |
| FD2114 | FastDigest CsiI | MabI, SexAI | A^CCWGGT TGGWCC^A ACCWGGT | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. |
| FD2134 | FastDigest PteI | BsePIm BssHII | G^CGCGC CGCGC^G GCGCGC | 5’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD2144 | FastDigest FokI | BstF5I, BtsCI, FokI | GGATGNNNNNNNNN^NNNN CCTACNNNNNNNNNNNNN^ GGATGNNNNNNNNNNNNN | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. |
| FD2154 | FastDigest RruI | BtuMI, NruI | TCG^CGA AGC^GCT TCGCGA | blunt | 37° C | No (column purification) | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
| FD2164 | FastDigest KflI | Sse1825I, SanDI | GG^GWCCC CCCWG^GG GGGWCCC | 5’ protruding ends | 37° C | No (column purification) | Dam: no effect. CpG: blocked if overlapped. EcoKI: no effect. EcoBI: no effect. |
| FD2174 | FastDigest SaqAI | MseI, Tru1I, Tru9I | T^TAA AAT^T TTAA | 5’ protruding ends | 37° C | 65°C, 5 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoBI: no effect. |
| FD2184 | FastDigest BfoI | AccB2I, Bme142I, BsmHI, Bsp143II, Bst16I, Bst1473II, BstH2I, Btu34II, HaeII, HinHI, LpnI, NgoAI, NgoBI, NgoCI, NgoGI, NgoJI, NgoMI, NgoWI | RGCGC^Y Y^CGCGR RGCGCY | 3’ protruding ends | 37° C | 65°C, 10 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. |
| FD2204 | FastDigest PacI | TTAAT^TAA AAT^TAATT TTAATTAA | 3’ protruding ends | 37° C | 65°C, 10 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoBI: no effect. | |
| FD2224 | FastDigest PspFI | CCCAG^C G^GGTCG CCCAGC | 3’ protruding ends | 37° C | 80°C, 5 min | Dam: no effect. Dcm: no effect. CpG: impaired if overlapped. EcoKI: no effect. EcoBI: no effect. |
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