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TRIzol RNA Extraction Reagents |
TRIzol reagent is a widely used chemical solution for the extraction of RNA, DNA, and proteins from biological samples. It is a monophasic solution of phenol and guanidine isothiocyanate, which facilitates the breakdown of cells and the denaturation of proteins. TRIzol reagent works through a series of steps to isolate high-quality RNA from biological samples. Initially, when a sample is mixed with TRIzol, the cells are lysed, releasing cellular components into the solution. The phenol and guanidine isothiocyanate in TRIzol disrupt the cell membrane and denature proteins, protecting RNA from degradation by RNases. Following homogenization, chloroform is added, and the mixture is centrifuged, resulting in three distinct phases: the aqueous phase containing RNA, the interphase containing DNA, and the organic phase containing proteins and other cellular components. The RNA from the aqueous phase is then precipitated by mixing with isopropanol and collected via centrifugation. The resulting RNA pellet is washed with ethanol to remove impurities and dissolved in RNase-free water or buffer for further use. This effective process makes TRIzol reagent a versatile tool in molecular biology for isolating RNA, DNA, and proteins from a single sample.
RNA extraction reagent selection guide
| Product |  TRIzol Reagent |  TRIzol LS Reagent |  TRIzol Plus RNA Purification Kit |  TRIzol Max Bacterial RNA Isolation Kit |
|---|---|---|---|---|
| Description | Isolates total RNA from tissues or cells, including lipid-rich and difficult samples. | Designed for use with liquid samples, such as serum or virus preparations. | Offers rapid isolation of total RNA by combining the lysis capability of TRIzol Reagent with the convenient RNA extraction technology of the Invitrogen PureLink RNA Mini Kit silica spin column. | Offers rapid isolation of total RNA from gram-positive and gram-negative bacteria by combining the lysis capability of TRIzol Reagent with Max Bacterial Enhancement Reagent to inactivate endogenous RNases and promote protein denaturing. |
| Primary sample type | Bacteria, blood, cells, plant samples, tissue, viral samples, yeast | Blood, liquid samples (e.g., serum), viral samples | Bacteria, cells, plant samples, tissue, yeast | Bacteria |
| Final product | Total RNA, transcriptome RNA, or micro RNA | Total RNA, transcriptome RNA, or micro RNA | Total RNA, transcriptome RNA, or micro RNA | Total RNA, transcriptome RNA, or micro RNA |
| Product size | 100 mL or 200 mL | 100 mL or 200 mL | 100 mL or 200 mL | 100 preps or 200 preps |
| Purification time | 1 hour | 1 hour | 1 hour | 1 hour |
| Applications | Cloning, northern blotting, nuclease protection assays, real-time quantitative PCR (qPCR), reverse transcriptase PCR (RT-PCR), cDNA library construction | Cloning, northern blotting, nuclease protection assays, real-time quantitative PCR (qPCR), reverse transcriptase PCR (RT-PCR), cDNA library construction | Microarray analysis, northern blotting, nuclease protection assays, real-time quantitative PCR (qPCR), reverse transcriptase PCR (RT-PCR), cDNA library construction | Cloning, northern blotting, nuclease protection assays, real-time quantitative PCR (qPCR), reverse transcriptase PCR (RT-PCR), cDNA library construction |
Meet a great match for TRIzol reagents: Phasemaker Tubes
Using TRIzol reagents just got simpler, thanks to Phasemaker Tubes creating a tight, durable seal between the aqueous and organic phase of your TRIzol mix. Phasemaker Tubes don’t compromise the performance of TRIzol reagents, helping you to easily pipette off the RNA phase. Benefits of using Phasemaker Tubes include:
- Higher yield—Increase recovery by as much as 30%
- Ease of use—Use with TRIzol mix, centrifuge, and then easily pipette off the aqueous phase
- Ready-to-use convenience—Comes pre-dispensed in 2 mL tubes
- Increased throughput—Stable gel seal helps maximize the number of samples per run without losing phase separation
Featured videos using TRIzol reagents
Video: TRIzol's new best friend: Phasemaker Tubes
Phasemaker Tubes create a durable seal between the aqueous and organic phases, allowing researchers to easily pipette off the RNA phase.
Video: How to isolate RNA from tissue or cells
Learn how to isolate ultrapure total RNA within an hour, even from difficult samples with the TRIzol Plus RNA Purification Kit.
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For Research Use Only. Not for use in diagnostic procedures.