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Cryopreservation Information
Use our tools and resources to help improve cryopreservation skills in the lab and learn about cryopreservation of cells.
What is cyropreservation?
Cryopreservation is the process of freezing biological samples, such as cells, tissues, or organs, at extremely low temperatures to preserve their viability for future use. Cryopreservation is significant because it helps enable long-term storage and preservation of biological samples, helping ensure their availability for future research, medical treatments, and reproductive technologies.
Cryopreservation of cells in cell culture is the process of freezing and storing cultured cells at ultra-low temperatures to maintain their viability and functionality for future experimental or therapeutic use.
Good recovery relies on successful storage
Preparation is key. Improper cryogenic preservation can have disastrous outcomes, leading to poor (or no!) cell recovery and project setbacks. Review information on cryopreservation and cold storage best practices before you freeze your first vial.
Cryopreservation
Use the tools and resources on this page to learn more about cryopreservation. Read guides or watch webinars that teach you common best practices regarding freezing cells and cold storage.
Cryopreservation features
Handbook
Written as a collaborative effort between authors from Thermo Fisher Scientific and American Type Culture Collection (ATCC), this comprehensive guide gives practical information on sample preparation, cryo storage, and recovery of cultured cells.
Webinar
This webinar will provide detail related to cell preservation and storage considerations, as well as criteria for selecting appropriate storage tubes to meet your downstream cell culture needs.
Webinar: Choosing the right cold storage for cell culture applications
Learn how to select the best cold storage and sample prep solutions, with an eye to pertinent criteria that can lead to an optimized cell culture experience.
Videos on cryopreservation
Video: Freezing cells
This video demonstrates the critical steps required to freeze cells while maintaining optimal cell health. We review the equipment required, how to prepare for freezing, and each step performed in a careful way at the right pace to prevent damage to your cells.
Video: Thawing and plating cryopreserved hepatocytes
This video covers the thawing and prep of cryopreserved hepatocytes for their subsequent use in applications such as metabolic stability (intrinsic clearance), metabolite ID/profiling, enzyme induction, hepatotoxicity, transporter uptake and efflux, environmental bioaccumulation, and liver disease.
Cell Culture & Transfection Resource Library
Browse cell culture & transfection learning center content by collection (resource type)—application notes, case studies, videos, webinars, and white papers.