Protein Concentrators

Protein extraction, purification, and cleanup methods frequently produce dilute samples of target biomolecules in buffer matrices incompatible with downstream applications. Pierce protein concentrators are disposable ultrafiltration centrifugal devices used to concentrate, desalt, and buffer exchange biological samples, such as proteins and nucleic acids, readying them for downstream applications with minimal sample handling. Pierce protein concentrators are available in four sizes (0.5, 6, 20, and 100 mL) and offer pH compatibility over a broad pH range (1–9).

The Nanocon™ Crossflow Concentrator is an innovative solution for exosome, lipid nanoparticle (LNP), and virus enrichment. The 30 nM pore size PE membrane is perfect for nanosized particles, and it fits conveniently in a 250 mL centrifuge adapter.

For larger volume sizes, Pierce crossflow filtration cassettes are designed for high purity and recovery of peptides, proteins, and virus/viral particles. They are used to concentrate large supernatant volumes (0.5 L–6 L). Additional accessories are available for use with the crossflow filtration cassettes.

• Fast—concentrate samples up to 10- to 50-fold in 5–30 minutes (times may vary with different MWCOs)
• Flexible—suitable for sample volumes ranging from 100 μL to 100 mL, with a wide range of MWCO offerings
• Efficient—recovery of >90% of target sample while removing contaminants or exchange buffers
• Convenient—clear markings, wide sample chamber, and removable filtrate chamber make handling simple and easy
• Instrument compatible—can be used with standard centrifuges with either fixed-angle or swinging-bucket rotors

• Scalable—concentrate sample volumes ranging from 0.5–6 L
• Fast—many concentration and protein recovery protocols can be completed in 30 or less minutes
• Versatile—concentration cassettes are available in five different MWCOs and are compatible with buffers up to pH 13
• Efficient—recovery of >99% protein (with 25 mL buffer rinse)

0.1–0.5 mL concentrators
Watch how to concentrate protein solution for sample volumes up to 0.5 mL using the microcentrifuge tube format. Membranes are available with 3K, 10K, 30K, 50K, and 100K MWCO.

2–6 mL concentrators
Watch how to concentrate protein solution for sample volumes up to 6 mL using the 15 mL centrifuge tube format. Membranes are available with 3K, 10K, 30K, 50K, or 100K MWCO.

5–20 mL concentrators
Watch how to concentrate protein solution for sample volumes up to 20 mL using the 20 mL tube format. Membranes are available with 3K, 10K, 30K, 50K, and 100K MWCO.

20–100 mL concentrators
Watch how to concentrate protein solution for sample volumes up to 100 mL using the 250 mL centrifuge bottle format. Membranes are available with 5K, 10K, 30K, 50K, and 100K MWCO.

0.5–6 L concentrators
Watch how to concentrate protein solution for sample volumes up to 6 liters using the Crossflow Filtration Cassette. These tangential flow filtration (TFF) cassettes are available in 3K, 10K, 30K, 50K, and 100K MWCO.