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Zeba Spin Desalting Columns
Thermo Scientific Zeba Spin Desalting Columns are made of low protein-binding polypropylene and are compatible with a wide range of standard laboratory instruments and consumables.
Our Zeba columns are designed for use with most swinging-bucket or fixed-angle bench- or floor-model centrifuges (please verify head clearance before use), and are available in six volume capacities and two molecular weight cut-offs (MWCOs), 7K and 40K.
The MWCO should be lower than the molecular weight of the target molecule to help ensure it is retained while contaminants are removed. Columns with 7K help provide finer resolution for smaller molecules, useful for purifying smaller proteins (e.g., cytochrome c) or peptides (e.g., insulin). Columns with 40K are better suited for large proteins (e.g., BSA), protein complexes (e.g., hemoglobin) and large enzymes (e.g,. pyruvate kinase).
Choose the right Zeba spin desalting column with 7K MWCO
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| Sample volume | 2–12 µL | 30–130 µL | 200–700 µL | 500–2,000 µL | 700–4,000 µL | 4,000–10,000 µL |
| Resin bed volume | 75 μL | 0.5 mL | 2 mL | 5 mL | 10 mL | 20 mL |
| Product type | Micro Spin Column | 0.5 mL Spin Column | 2 mL Spin Column | 5 mL Spin Column | 10 mL Spin Column | 20 mL Spin Column |
| See all 7K MWCO Zeba Spin Desalting Columns, Plates, and Cartridges › | ||||||
Choose the right Zeba spin desalting column with 40K MWCO
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| Sample volume | 5–14 µL | 70–200 µL | 200–900 µL | 300–2,000 µL | 1,000–4,000 µL | 4,000–10,000 µL |
| Resin bed volume | 75 μL | 0.5 mL | 2 mL | 5 mL | 10 mL | 20 mL |
| Product type | Micro Spin Column | 0.5 mL Spin Column | 2 mL Spin Column | 5 mL Spin Column | 10 mL Spin Column | 20 mL Spin Column |
| See all 40K MWCO Zeba Spin Desalting Columns, Plates, and Cartridges › | ||||||
Choose the right Zeba product for high throughput and automation
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| MWCO | 7K | 7K | 7K | 40K |
| Sample volume | 20–100 µL | 50–250 µL | 100–1,500 µL | 20–100 µL |
| Resin bed volume | N/A | 1 mL | 5 mL | N/A |
| Product type | 96-well filter plates | 1 mL Desalting Cartridges | 5 mL Desalting Cartridges | 96-well filter plates |
Additional products used to efficiently wash away contaminates and elute your purified sample:
- Pierce Micro-Spin Columns —400 μL column volume ideal for 5 to 100 μL protein purification
- Pierce Spin Columns – Screw Cap —900 μL column volume ideal for 20 to 400 μL protein purification
- Pierce Spin Columns – Snap Cap —1 mL column volume ideal for 20 to 500 μL protein purification
Zeba Spin Desalting Columns and Plates video
Thermo Scientific Zeba Spin Desalting Columns
Zeba spin desalting columns quickly and effectively remove salts and other small molecules from your sample using gel-filtration chromatography. They contain ready-to-use size exclusion resin that enables high salt retention and protein recovery. Zeba columns eliminate the need for gravity-filtration, thus speeding up the desalting process. View the video to learn how to desalt your sample using the Zeba Spin Desalting Columns.
Zeba spin desalting column performance
Zeba Spin Desalting Columns are available in a variety of sizes and volume capacities compared to competitor offerings (Table 1). Zeba columns can also be used with volumes as small as 5 uL (in micro format) to as large as 10 mL
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Table 1. Comparison of recommended sample volume capacity of common spin desalting products.
Zeba 40K Spin Desalting Columns contain a new and enhanced high-performance size-exclusion chromatography resin. These redeveloped Zeba columns demonstrate improved protein recovery for a variety of proteins in the 40 kDa and above molecular weight range (Table 2).
| Recovery* | |||
|---|---|---|---|
| Protein | Molecular Size | 7K Zeba Column | 40K Zeba Column |
| Bacitracin | 1.5 kDa | 15% | 12% |
| Aprotinin | 6.5 kDa | 58% | 37% |
| Ubiquitin | 8.5 kDa | 78% | 51% |
| Lactalbumin | 14 kDa | 88% | 64% |
| Trypsin Inhibitor | 21 kDa | 94% | 76% |
| Carbonic Anhydrase | 30 kDa | 90% | 79% |
| Ovalbumin | 42 kDa | 95% | 95% |
| BSA | 67 kDa | 94% | 93% |
| Rabbit IgG | 150 kDa | 92% | 92% |
*Recovery of 100 uL sample volume, with a loaded concentration of 0.5 mg/mL
Table 2. Protein recovery comparison using Zeba Protein Desalting Columns with 7K and 40K MWCO resin.
Zeba 7K Spin Desalting Columns contain a high-performance size-exclusion chromatography resin that allows for exceptional protein desalting and recovery. When compared to competitors, Zeba 7K desalting columns have superior protein recovery even at dilute protein concentrations (Figure 1).
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Figure 1. Zeba Micro Spin Desalting Columns, 7K MWCO, recovery performance. BSA and ubiquitin samples of varying concentrations were processed using Zeba Micro Spin Desalting Columns, 7K MWCO, alongside comparable competitor products. The data show that, even at very dilute protein concentrations (250 ng), the Zeba Desalting columns outperform those of the competitors, yielding higher protein recovery for both BSA and ubiquitin.
Zeba 40K Spin Desalting Columns provide superior performance that improves protein recovery compared to competitors (Figure 2). Further, the new resin in the Zeba 40K desalting columns allows for higher desalting and is more effective at small protein removal (Figure 3).
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Figure 2. Exceptional sample recovery of low protein loads with Zeba 40K spin columns. BSA and IgG of varying concentrations were applied in 50µl aliquots to either 0.5 mL Zeba 40K, competitor C, or Competitor B spin columns. All columns were equilibrated with PBS, then centrifuged per manufacturers’ instructions for each resin. Recovered protein (%) in each flow through sample was determined by BCA assay. Flow through samples were performed on SDS-PAGE and stained with either Coomassie Blue or silver stain. Although 12.5 µg total protein load was recovered efficiently by all three resins, lower recoveries were observed with competitor resins at 1 ug and 250 ng protein loads.
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Figure 3. New enhanced Zeba 40K resin is more effective than that of competitors at removing small proteins. Purified protein samples (0.5mg/mL) of varying molecular weights were applied individually in 50 ul aliquots in either 0.5 mL Zeba 40K, Competitor C, or Competitor B spin columns equilibrated in PBS. Columns were centrifuged per manufacturers’ instructions for each resin, and the recovered protein (%) in each flow-through sample was determined by BCA protein assay. As opposed to competitor columns, the Zeba 40K resin was more efficient at removing smaller proteins (evidenced by the lower small protein recovery values).
Zeba Desalting Handbook
Thermo Scientific Zeba Resin technology provides consistent performance and peace of mind. The unique, proprietary Zeba Resin enables better protein recovery, even for samples with low protein concentrations.
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