Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL
Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL
Thermo Scientific™

Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL

Perform high-throughput protein recovery (7K MWCO) and sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development with ready-to-use Thermo Scientific Pierce Zeba desalting chromatography columns, plates, and cartridges.
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Catalog NumberQuantityFormatVolume (Metric)
898072 Plates96-well Filter Plate96-well
8987725 ColumnsSpin Column75 μL
8987850 ColumnsSpin Column75 μL
A400024695 ColumnsSpin Column20 mL
A4000247510 ColumnsSpin Column20 mL
8989025 ColumnsSpin Column2 mL
898915 ColumnsSpin Column5 mL
8989225 ColumnsSpin Column5 mL
898935 ColumnsSpin Column10 mL
8989425 ColumnsSpin Column10 mL
898895 ColumnsSpin Column2 mL
8988225 ColumnsSpin Column0.5 mL
8988350 ColumnsSpin Column0.5 mL
899345 columns x 1 mLChromatography Cartridge1 mL
899351 column x 5 mLChromatography Cartridge5 mL
898084 Plates96-well Filter Plate96-well
Catalog number 89807
Price (USD)
322.65
Online Exclusive
367.00
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Quantity:
2 Plates
Format:
96-well Filter Plate
Volume (Metric):
96-well
Recurring order eligible. Learn more »
Request bulk or custom format
Price (USD)
322.65
Online Exclusive
367.00
Save 44.35 (12%)
Each
Add to cart
Ask our AI about this Product
Perform high-throughput protein recovery and sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development with ready-to-use Pierce Zeba desalting chromatography columns, plates, and cartridges. Rapidly desalt protein and biological samples for manual or automated liquid chromatography (LC) with ≥95% retention of salts and other small molecular contaminants (<1,000 Da).

These Zeba spin desalting products contain a proprietary high-performance size-exclusion chromatography resin that enables excellent protein desalting and recovery of 7K molecular weight cutoff (MWCO) molecules using a centrifuge format. This resin is ideal for removing low molecular weight compounds including salts, fluorescent dyes, biotin, and other small labelling reagents.

Benefits of Zeba desalting columns, plates, and cartridges include:

  • High recovery—low-binding resin maximizes protein recovery,
  • Fast—no fraction screening or waiting for protein to emerge by gravity flow
  • Economical—great performance at lower cost than other commercially available cartridges
  • Easy-to-use—no cumbersome column preparation or equilibration
  • Flexible—available in spin columns, filter spin plates, and cartridges for a range of needs 

Zeba Spin Desalting Columns 

Convenient, ready-to-use polypropylene columns are the ideal solution for quick and straightforward desalting and buffer exchange of protein samples in a centrifuge format. The pre-dispensed spin columns are offered in various resin fills (75 μL, 0.5 mL, 2 mL, 5 mL, 10 mL, 20 mL). These options make it simple to handle sample volumes between 2 μL and 10 mL.

Zeba Desalting Plates

Provide trouble-free high-throughput desalting and buffer exchange for sample volumes ranging from 20 to 100 μL. Pre-dispensed 96-well filter plates require no resin dispensing or hydration and provide the same high protein recovery as spin columns with at least 95% removal of salts and other small molecules. One plate can process 96 small sample volumes (20 to 100 μL) in five minutes. Compatible with centrifugation for manual or automated purification.

Zeba Desalting Chromatography Cartridges

Compatible with automated liquid chromatography systems and with syringe processing, Zeba desalting cartridges provide fast, easy, and reproducible chromatographic separations and can be regenerated for multiple uses.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeSpin Desalting Plate
Purification TargetBuffer Exchange, Protein
Quantity2 Plates
Volume (Metric)96-well
Column TypeSize-exclusion, Proprietary Resin
Format96-well Filter Plate
MWCO7.0 kDa
Product LineZeba™
Sample Volume (Metric)20 to 100 μL
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C.
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Frequently asked questions (FAQs)

Besides precipitation with ammonium sulfate, what are some ways I can concentrate my protein sample?

You may remove excess solvent and smaller moieties by centrifugation through a microconcentrator. This will concentrate your protein sample.

(1) Choose microconcentrator tube (available from a variety of commercial suppliers) with a protein cutoff smaller than the molecular weight of the protein in the sample. Check our Pierce Protein Concentrators PES.

(2) Add 1 µL of 20% w/v SDS to a dry microconcentrator tube (if sample does not already contain SDS).

(3) Slowly add sample (a few microliters at a time) to membrane until membrane is completely wet. Centrifuge to near (but not complete) dryness.

(4) If intention is to desalt sample or buffer exchange: Add ~50 µL water to microconcentrator and spin until nearly dry. Repeat buffer exchange. Sample will remain on membrane. Check our Zeba desalting proteins.

(5) Using a new collection tube, invert membrane and spin at low speed (1000 x g) to elute protein from membrane. Add 2X SDS-Sample Buffer containing 10 mM DTT to membrane: vortex, invert and spin. Final volume should be ~20 µL.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Can Zeba Desalting columns, plates, and cartridges be autoclaved?

Yes, Zeba Desalting columns, plates, and cartridges can be autoclaved at 115 degrees C for 30 min.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

What reagents and conditions are Zeba Desalting columns, plates, and cartridges compatible with?

Zeba Desalting columns, plates, and cartridges are compatible with a range of reagents and conditions. They have been confirmed to be compatible with the following:
- pH range of 3-10
- Mild oxidants/reductants
- Chaotropes (stable in 8 M urea and 6 M guanidine HCl)
- Salts
- Alcohols up to 20%
- Organic solvents such as dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) (we recommend using a step-gradient of increasing concentration of the organic solvent during column equilibration)

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Can I use Zeba Desalting plates with a vacuum manifold, and if so, are there any specific considerations or limitations to keep in mind?

Yes, Zeba Desalting plates can be used with a vacuum manifold. Unfortunately, we do not offer a protocol for the same. It is important to note that the pressure settings of the vacuum manifold will need to be optimized for each instrument as the pressure apparatus may differ. Additionally, it has been reported that protein recovery with Zeba Desalting plates using a vacuum manifold may be lower compared to the traditional centrifugation workflow.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

How should I store Zeba Spin Desalting Columns, Plates, and Cartridges?

We recommend storing Zeba Spin Desalting Columns, Plates, and Cartridges at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

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Zeba Protein Desalting Spin Columns and 96-Well Spin Plates

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      Citations & References (15)

      Citations & References
      Abstract
      Cardioprotective O-GlcNAc signaling is elevated in murine female hearts via enhanced O-GlcNAc transferase activity.
      Authors:Narayanan B,Sinha P,Henry R,Reeves RA,Paolocci N,Kohr MJ,Zachara NE
      Journal:The Journal of biological chemistry
      PubMed ID:37949223
      The post-translational modification of intracellular proteins by O-linked β-GlcNAc (O-GlcNAc) has emerged as a critical regulator of cardiac function. Enhanced O-GlcNAcylation activates cytoprotective pathways in cardiac models of ischemia-reperfusion (I/R) injury; however, the mechanisms underpinning O-GlcNAc cycling in response to I/R injury have not been comprehensively assessed. The cycling of ... More
      Ethinylestradiol in combined hormonal contraceptive has a broader effect on serum proteome compared with estradiol valerate: a randomized controlled trial.
      Authors:Kangasniemi MH,Arffman RK,Joenväärä S,Haverinen A,Luiro K,Tohmola T,Renkonen R,Heikinheimo O,Tapanainen JS,Piltonen TT
      Journal:Human reproduction (Oxford, England)
      PubMed ID:36416543
      STUDY QUESTION: Does an estradiol-based combined oral contraceptive (COC) have a milder effect on the serum proteome than an ethinylestradiol (EE)-based COC or dienogest (DNG) only? SUMMARY ANSWER: The changes in serum proteome were multifold after the use of a synthetic EE-based COC compared to natural estrogen COC or progestin-only ... More
      Antibody-dependent enhancement of toxicity of myotoxin II from Bothrops asper.
      Authors:Sørensen CV,Fernández J,Adams AC,Wildenauer HHK,Schoffelen S,Ledsgaard L,Pucca MB,Fiebig M,Cerni FA,Tulika T,Voldborg BG,Karatt-Vellatt A,Morth JP,Ljungars A,Grav LM,Lomonte B,Laustsen AH
      Journal:Nature communications
      PubMed ID:38228619
      Improved therapies are needed against snakebite envenoming, which kills and permanently disables thousands of people each year. Recently developed neutralizing monoclonal antibodies against several snake toxins have shown promise in preclinical rodent models. Here, we use phage display technology to discover a human monoclonal antibody and show that this antibody causes antibody-dependent ... More
      Ubiquitin-driven protein condensation stabilizes clathrin-mediated endocytosis.
      Authors:Yuan F,Gollapudi S,Day KJ,Ashby G,Sangani A,Malady BT,Wang L,Lafer EM,Huibregtse JM,Stachowiak JC
      Journal:PNAS nexus
      PubMed ID:39253396
      Clathrin-mediated endocytosis is an essential cellular pathway that enables signaling and recycling of transmembrane proteins and lipids. During endocytosis, dozens of cytosolic proteins come together at the plasma membrane, assembling into a highly interconnected network that drives endocytic vesicle biogenesis. Recently, multiple groups have reported that early endocytic proteins form ... More
      Microscale Thermophoresis (MST) as a Tool to Study Binding Interactions of Oxygen-Sensitive Biohybrids.
      Authors:Jagilinki BP,Willis MA,Mus F,Sharma R,Pellows LM,Mulder DW,Yang ZY,Seefeldt LC,King PW,Dukovic G,Peters JW
      Journal:Bio-protocol
      PubMed ID:39131194
      Microscale thermophoresis (MST) is a technique used to measure the strength of molecular interactions. MST is a thermophoretic-based technique that monitors the change in fluorescence associated with the movement of fluorescent-labeled molecules in response to a temperature gradient triggered by an IR LASER. MST has advantages over other approaches for ... More
      15 total citations

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