Search Thermo Fisher Scientific
Certificates
SDS
Citations & References (1)
Invitrogen™
Terminal Deoxynucleotidyl Transferase (TdT) Buffer
Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3fi hydroxyl terminusRead more
| Catalog Number | Quantity |
|---|---|
| 16314015 | 2 x 0.8 mL |
Catalog number 16314015
Price (USD)
88.75
Each
-
Quantity:
2 x 0.8 mL
Price (USD)
88.75
Each
Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3fi hydroxyl terminus of DNA. A TdT Technical Bulletin is available.
Applications: Homopolymer tailing of vector and insert for cloning. Labeling oligonucleotides with biotin (1,2), 32P- or 35S-label (3), or in apoptosis (TUNEL) (4,5).
Source: Purified from a baculovirus clone of calf thymus TdT.
Performance and Quality Testing: Endonuclease, 3´ and 5´ exodeoxyribonuclease, and levels of incorporation tested.
Unit Definition: One unit incorporates 1 nmol dATP into acid-precipitable material in 1 h at 37°C, using d(pA)50 as a primer.
Hazard Warning: Toxic; potassium cacodylate contained in reaction buffer. Also contains cobalt chloride, a highly toxic chemical.
See MSDS.
Unit Reaction Conditions: 0.2 M potassium cacodylate (pH 7.2), 10 mM MgO4 C4 H6 , 1 mM 2-mercaptoethanol, 0.5 mg/ml BSA,
100 flM d(pA)50, 1 mM [3H]dATP, and enzyme in 0.15 ml for 1 h at 37°C.
Applications: Homopolymer tailing of vector and insert for cloning. Labeling oligonucleotides with biotin (1,2), 32P- or 35S-label (3), or in apoptosis (TUNEL) (4,5).
Source: Purified from a baculovirus clone of calf thymus TdT.
Performance and Quality Testing: Endonuclease, 3´ and 5´ exodeoxyribonuclease, and levels of incorporation tested.
Unit Definition: One unit incorporates 1 nmol dATP into acid-precipitable material in 1 h at 37°C, using d(pA)50 as a primer.
Hazard Warning: Toxic; potassium cacodylate contained in reaction buffer. Also contains cobalt chloride, a highly toxic chemical.
See MSDS.
Unit Reaction Conditions: 0.2 M potassium cacodylate (pH 7.2), 10 mM MgO4 C4 H6 , 1 mM 2-mercaptoethanol, 0.5 mg/ml BSA,
100 flM d(pA)50, 1 mM [3H]dATP, and enzyme in 0.15 ml for 1 h at 37°C.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Compatible Buffer5X Buffer, Reaction Buffer
Product TypeTdT
Quantity2 x 0.8 mL
Shipping ConditionApproved for shipment on Wet or Dry Ice
Unit SizeEach
Contents & Storage
Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is supplied with vial of 5X buffer [500 mM potassium cacodylate (pH 7.2), 10 mM CoCl2 , 1 mM DTT]. Store at -20°C.
Have questions about this product? Ask our AI assisted search.
What is the stability of Thermo Scientific restriction enzymes if they freeze-thaw during shipment?
Can I double digest my DNA using a Thermo Scientific conventional restriction enzyme and a FastDigest restriction enzyme?
Do Thermo Scientific restriction enzymes come with the buffer(s)?
Why do you recommend only 2 µL of 10X Reaction Buffer when digesting unpurified PCR product in a 30 µL reaction?
What are key factors promoting star activity?
Show more FAQs for this product
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our
Privacy Notice.
Los clientes que vieron este artículo también vieron
Documents & Downloads
Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
2947667Certificate of AnalysisJun 25, 202416314-015, 16314015
2748692Certificate of AnalysisOct 06, 202316314015
2563721Certificate of AnalysisApr 11, 202316314015
2525138Certificate of AnalysisSep 30, 202216314-015, 16314015
1652941Certificate of AnalysisJul 21, 201716314015
5 results displayed, search above for a specific certificate
Safety Data Sheets
SDSScientific Resources
Citations & References (1)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Different effects on human topoisomerase I by minor groove and intercalated deoxyguanosine adducts derived from two polycyclic aromatic hydrocarbon diol epoxides at or near a normal cleavage site.
Journal:J Biol Chem
PubMed ID:11832494
Topoisomerase I (top1) relieves supercoiling in DNA by forming transient covalent cleavage complexes. These cleavage complexes can accumulate in the presence of damaged DNA or anticancer drugs that either intercalate or lie in the minor groove. Recently we reported that covalent diol epoxide (DE) adducts of benzo[a]pyrene (BaP) at the
1 total citations