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Gibco™
MEM, powder
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be usedRead more
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| Catalog Number | Quantity |
|---|---|
| 61100061 | 10 x 1 L |
| 61100087 | 10 L |
| 61100103 | 50 L |
Catalog number 61100061
Price (USD)
59.65
Online Exclusive
62.00Save 2.35 (4%)
Each
In stock
Quantity:
10 x 1 L
Price (USD)
59.65
Online Exclusive
62.00Save 2.35 (4%)
Each
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM is modified as follows:
| With | Without |
| • L-glutamine | • HEPES |
| • Phenol Red | • Sodium Bicarbonate |
The complete formulation is available.
Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts.
Using MEM
MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
Specifications
Cell LineHeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell TypePrimary Rat Astrocytes
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeMEM (Minimum Essential Medium)
Quantity10 x 1 L
Shelf Life36 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormPowder
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
With AdditivesLow Glucose, Glutamine, Phenol Red
Without AdditivesNo HEPES, No Sodium Pyruvate, No Sodium Bicarbonate
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Media Formulations
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Lot #Certificate TypeDateCatalog Number(s)
3220776Certificate of AnalysisJun 26, 202561100087, 61100053, 61100103, 61100061
3219049Certificate of AnalysisMay 29, 202561100087, 61100053, 61100103, 61100061
3158130Certificate of AnalysisMay 07, 202561100087, 61100053, 61100103, 61100061
3124554Certificate of AnalysisApr 23, 202561100087, 61100053, 61100103, 61100061
3050957Certificate of AnalysisApr 05, 202561100087, 61100053, 61100103, 61100061
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SDSCitations & References (1)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Hsp105alpha suppresses the aggregation of truncated androgen receptor with expanded CAG repeats and cell toxicity.
Journal:J Biol Chem
PubMed ID:12714591
Spinal and bulbar muscular atrophy (SBMA) is a neurodegenerative disorder caused by the expansion of a polyglutamine tract in the androgen receptor (AR). The N-terminal fragment of AR containing the expanded polyglutamine tract aggregates in cytoplasm and/or in nucleus and induces cell death. Some chaperones such as Hsp40 and Hsp70
1 total citations