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PBS (10X), pH 7.4
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PBS (10X), pH 7.4
Gibco™

PBS (10X), pH 7.4

Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such asRead more
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Catalog NumberQuantity
70011044Promo Image500 mL
70011069Promo Image10 x 500 mL
Catalog number 70011044
Price (USD)
67.75
Each
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500 mL
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Price (USD)
67.75
Each
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Ask our AI about this Product

Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution (see protocol for details).

We offer a variety of Gibco™ PBS formulations for a range of cell culture applications.


This PBS is manufactured as follows:
Without
• Calcium
• Magnesium
• Phenol Red


The complete formulation is available.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
AdditiveNo Sodium Pyruvate
ClassificationAnimal Origin-Free
ColorWhite
Dilution10 X
For Use With (Application)Mammalian Cell Culture
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality2800 - 3100 mOsm/kg
Phenol Red IndicatorNo Phenol Red
Product LineGibco™
Quantity500 mL
Shipping ConditionRoom Temperature
SterilitySterile-filtered
FormLiquid
Product TypePhosphate Buffered Saline (PBS)
Solution TypePhosphate Buffered Saline
pH7.4
Unit SizeEach
Contents & Storage
Storage conditions: 15°C to 30°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
Media Formulations
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Frequently asked questions (FAQs)

Are your PBS products tested for endotoxin levels?

Our PBS, pH 7.2 and PBS, pH 7.4 products are tested for endotoxin and the endotoxin levels are shown on the Certificate of Analysis (CoA) for the particular lot number of the product.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your PBS products sterile and suitable for cell culture?

We offer PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free (Cat. Nos. AM9624 and AM9625), which is molecular biology grade and is certified to be RNase-free. However, it is not sterile and hence is not cell culture-grade.

All our other PBS products (Cat. Nos. 70011xxx, 10010xxx, 70013xxx, 20012xxx) are filter-sterilized using a 0.1 µm filter and are cell culture grade (ready to use directly on cells). However, they are not tested for nuclease activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your PBS products DNase- and RNase-free?

We offer PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free (Cat. Nos. AM9624 and AM9625) that is molecular biology grade and is certified to be RNase-free. Our other PBS products are made with distilled water and are filter-sterilized, but are not tested for RNase and DNase activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is a precipitate in PBS normal?

No, this is not normal and if there is a precipitate in the product, it cannot be used. If the precipitate will not go back into solution, please contact Technical Support at techsupport@thermofisher.com for a replacement.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between PBS and DPBS?

PBS has no potassium chloride (KCl) and it has a slightly lower level of phosphate (PO4) than DPBS. PBS is used for washing cells when viability isn't critical.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3149835Certificate of AnalysisJul 05, 202570011036, 70011044
3141726Certificate of AnalysisJun 01, 202570011036, 70011044
3138542Certificate of AnalysisMay 04, 202570011036, 70011044
3090920Certificate of AnalysisApr 25, 202570011036, 70011044
3136935Certificate of AnalysisApr 02, 202570011036, 70011044
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Safety Data Sheets

Citations & References (4)

Citations & References
Abstract
Subcellular targeting of RGS9-2 is controlled by multiple molecular determinants on its membrane anchor, R7BP.
Authors:Song JH, Waataja JJ, Martemyanov KA,
Journal:J Biol Chem
PubMed ID:16574655
'RGS9-2, a member of the R7 RGS protein family of neuronal RGS (Regulators of G protein Signaling), is a critical regulator of G protein signaling. In striatal neurons, RGS9-2 is tightly associated with a novel palmitoylated protein - R7BP (R7 family Binding Protein). Here we report that R7BP acts to ... More
Inhibition of transforming growth factor beta signaling and Smad-dependent activation of transcription by the Latent Membrane Protein 1 of Epstein-Barr virus.
Authors: Prokova Vassiliki; Mosialos George; Kardassis Dimitris;
Journal:J Biol Chem
PubMed ID:11781310
'Inhibition of transforming growth factor beta (TGFbeta) signaling by the Epstein-Barr virus Latent Membrane Protein 1 (LMP1) may account, at least in part, for the oncogenic activity of LMP1. We found that LMP1 is a potent inhibitor of TGFbeta signaling and Smad-dependent activation of transcription in 293 epithelial cells and ... More
Stiffness-controlled three-dimensional extracellular matrices for high-resolution imaging of cell behavior.
Authors:Fischer RS, Myers KA, Gardel ML, Waterman CM,
Journal:Nat Protoc
PubMed ID:23099487
Regulation of cell functions by the physical properties of the extracellular matrix (ECM) has emerged as a crucial contributor to development and disease. Two specific physical properties of the ECM, stiffness and dimensionality, each influence cell signaling and function. As these ECM physical properties are linked to other properties that ... More
Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures.
Authors:Zhang F, Gradinaru V, Adamantidis AR, Durand R, Airan RD, de Lecea L, Deisseroth K,
Journal:Nat Protoc
PubMed ID:20203662
Elucidation of the neural substrates underlying complex animal behaviors depends on precise activity control tools, as well as compatible readout methods. Recent developments in optogenetics have addressed this need, opening up new possibilities for systems neuroscience. Interrogation of even deep neural circuits can be conducted by directly probing the necessity ... More
4 total citations

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